It is evident that biochemical control is not strictly hierarchical and that intermediary metabolism can contribute to control of regulatory pathways. Metabolic studies are therefore increasingly important in gene function analyses, and an increased interest in metabolites as biomarkers for disease progression or response to therapeutic intervention is also evident in the pharmaceutical industry. This book offers guidelines to currently available technology and bioinformatics and database strategies now being developed. Evidence is presented that metabolic profiling is a valuable addition to genomics and proteomics strategies devoted to drug discovery and development, and that metabolic profiling offers numerous advantages.
Contributors. Preface. 1. Introduction; G.G. Harrigan, R. Goodacre. 2. Metabolome and Proteome Profiling for Microbial Characterization; S. Vaidyanathan, R. Goodacre. 3. Can 1H NMR Derived Metabolic Profiles Contribute to Proteomic Analyses? J.L. Griffin, E. Sang. 4. Application of Metabonomics in the Pharmaceutical Industry; A.P. Breau, G.H. Cantor. 5. Metabolic Profiling in Tumors by in Vivo and in Vitro NMR Spectroscopy; Yeun-Li Chung, M. Stubbs, J. Griffiths. 6. Raman Spectroscopy for Whole Organism and Tissue Profiling; S. Clarke, R. Goodacre. 7. Metabolic Fingerprinting with Fourier Transform Infrared Spectroscopy; D.I. Ellis, G.G. Harrigan, R. Goodacre. 8. Metabolomic Analysis with with Fourier Transform Ion Cyclotron Resonance Mass Spectroscopy; D. Goodenowe. 9. Stable Isotope-Based Dynamic Metabolic Profiling in Disease and Health; L.G. Boros, M. Cascante, W.-N.P. Lee. 10. The role of Metabolomics in Systems Biology; J.van der Greef, E. Davidov, E. Verheij, J. Vogels, R.van der Heijden, A.S. Adourian, M. Oresic, E.W. Marple, S. Naylor. 11. Use of Metabolomics to Discover Metabolic Patterns Associated with Human Diseases; O. Fiehn, J. Spranger. 12. Bioactive Lipids in Reproductive Diseases; J. Chaudhary, T. Compton, J.A. Parrott. 13. Evolutionary Computation for the Interpretation of Metabolomic Data; R. Goodacre, D.B. Kell. 14. Dynamic Profiling and Canonical Modeling; E.O. Voit, J. Almeida. 15. Databases, Data Modeling and Schemas; N. Hardy, H. Fuell. 16. Databases and Visualization for Metabolomics; X. Jing Lee, O. Brazhnik, A. Kamal, D. Guo, C. Lee, S. Hoops, P. Mendes. 17. The Human Metabolome. C.W.W. Beecher. Index.