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Life Sciences - Biochemistry & Biophysics | Nucleic Acids Hybridization - Modern Applications

Nucleic Acids Hybridization

Modern Applications

Buzdin, Anton, Lukyanov, Sergey (Eds.)

2007, XIX, 314 p.

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  • In-depth review of nucleic acid hybridization in vitro techniques
  • Covers all major applications
  • Includes novel methods that have the potential of being widely used
  • Provides detailed experimental protocols

Watson–Crick hybridization of complementary sequences in nucleic acids is one of the most important processes necessary for molecular recognition in vivo, as well as nucleic acid identification and isolation. This book is devoted to a large family of in vitro DNA hybridization-based experimental techniques. Several approaches, such as microarray hybridization, have become extremely popular tools for specialists in biochemistry and in biomedicine, while the potential of many other advantageous techniques seems to be currently underestimated. Written by an international team of authors, this book details the current state-of-art in hybridization techniques. It also helps readers in choosing an appropriate method for performing an experiment in the most efficient way. Coverage includes experimental protocols along with comprehensive and detailed method descriptions. This truly universal book will be useful to all those interested in the modern life science methodologies.

Content Level » Research

Keywords » DNA - Microarray - Mutation - PCR - Promoter - evolution - hybridization - molecular biology

Related subjects » Biochemistry & Biophysics - Cancer Research - Human Genetics - Virology

Table of contents 

Contributing Authors Preface Acknowledgements Nucleic acids hybridization: potentials and limitations Anton Buzdin Selective suppression of polymerase chain reaction and its most popular applications Sergey Lukyanov, Konstantin Lukyanov, Nadezhda Gurskaya, Ekaterina Bogdanova, Anton Buzdin Suppression subtractive hybridization Sergey Lukyanov, Denis Rebrikov, Anton Buzdin Stem-loop oligonucleotides as hybridization probes and their practical use in molecular biology and biomedicine Anton Buzdin, Sergey Lukyanov Generation of normalized cDNA libraries Alex Shcheglov, Pavel Zhulidov, Ekaterina Bogdanova, Dmitry Shagin Primer extension enrichment reaction (PEER) and other methods for difference screening Lilia Ganova-Raeva Subtractive hybridization with covalently modified oligonucleotides Shi-Lung Lin, Donald Chang, Joseph Miller and Shao-Yao Ying Coincidence cloning: robust technique for isolation of common sequences Anton Buzdin DNA hybridization in solution for mutation detection Anton Buzdin Current attempts to improve the specificity of nucleic acids hybridization Anton Buzdin Concepts on microarray design for genome and transcriptome analyses Helder I. Nakaya, Eduardo M. Reis and Sergio Verjovski-Almeida Index

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