Springer eBooks may be purchased by end-customers only and are sold without copy protection (DRM free). Instead, all eBooks include personalized watermarks. This means you can read the Springer eBooks across numerous devices such as Laptops, eReaders, and tablets.
You can pay for Springer eBooks with Visa, Mastercard, American Express or Paypal.
After the purchase you can directly download the eBook file or read it online in our Springer eBook Reader. Furthermore your eBook will be stored in your MySpringer account. So you can always re-download your eBooks.
In-depth review of nucleic acid hybridization in vitro techniques
Covers all major applications
Includes novel methods that have the potential of being widely used
Provides detailed experimental protocols
Watson–Crick hybridization of complementary sequences in nucleic acids is one of the most important processes necessary for molecular recognition in vivo, as well as nucleic acid identification and isolation. This book is devoted to a large family of in vitro DNA hybridization-based experimental techniques. Several approaches, such as microarray hybridization, have become extremely popular tools for specialists in biochemistry and in biomedicine, while the potential of many other advantageous techniques seems to be currently underestimated. Written by an international team of authors, this book details the current state-of-art in hybridization techniques. It also helps readers in choosing an appropriate method for performing an experiment in the most efficient way. Coverage includes experimental protocols along with comprehensive and detailed method descriptions. This truly universal book will be useful to all those interested in the modern life science methodologies.
Acknowledgements Nucleic acids hybridization: potentials and limitations
Anton Buzdin Selective suppression of polymerase chain reaction and its most popular applications
Sergey Lukyanov, Konstantin Lukyanov, Nadezhda Gurskaya, Ekaterina Bogdanova, Anton Buzdin Suppression subtractive hybridization
Sergey Lukyanov, Denis Rebrikov, Anton Buzdin Stem-loop oligonucleotides as hybridization probes and their practical use in molecular biology and biomedicine
Anton Buzdin, Sergey Lukyanov Generation of normalized cDNA libraries
Alex Shcheglov, Pavel Zhulidov, Ekaterina Bogdanova, Dmitry Shagin Primer extension enrichment reaction (PEER) and other methods for difference screening
Lilia Ganova-Raeva Subtractive hybridization with covalently modified oligonucleotides
Shi-Lung Lin, Donald Chang, Joseph Miller and Shao-Yao Ying Coincidence cloning: robust technique for isolation of common sequences
Anton Buzdin DNA hybridization in solution for mutation detection
Anton Buzdin Current attempts to improve the specificity of nucleic acids hybridization
Anton Buzdin Concepts on microarray design for genome and transcriptome analyses
Helder I. Nakaya, Eduardo M. Reis and Sergio Verjovski-Almeida Index