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Life Sciences - Biochemistry & Biophysics | Capillary Electrophoresis of Nucleic Acids

Capillary Electrophoresis of Nucleic Acids

Series: Methods in Molecular Biology, Vol. 163

Mitchelson, Keith R., Cheng, Jing (Eds.)

2001, XX, 408 p.

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The advent of capillary electrophoresis (CE) , with its power to separate and analyze very small amounts of DNA, has produced a host of DNA analytical procedures. In the
Unprecedentedly wide-ranging volumes of Capillary Electrophoresis of Nucleic Acids, an outstanding panel of hands-on experts and developers of CE equipment describe in step-by-step fashion their best cutting-edge methods for the detection and analysis of DNA mutations and modifications, ranging from precise DNA loci to entire genomes of organisms. This second volume of the set, Practical Applications of Capillary Electrophoresis, covers techniques for high-throughput analysis of DNA fragments using SNP detection, mutation detection, DNA sequencing methods, and DNA-ligand interactions. Several chapters also discuss CE analysis at elevated temperatures and the use of micro-CE and array-CE devices. The companion volume, Introduction to the Capillary Electrophoresis of Nucleic Acids, offers readily reproducible methods for the analysis of small oligonucleotides and modified nucleotides, and time-tested advice on instrumentation, signal detection, the capillary environment, and the integration of mass spectrometry with CE.
Comprehensive and up-to-date, the paired volumes of Capillary Electrophoresis of Nucleic Acids offer an authoritative guide with easy access to fast, versatile, reliable, and powerful technologies for all those basic and clinical investigators analyzing DNA variation today.

Content Level » Professional/practitioner

Keywords » DNA - Nucleotide - PCR - RNA - fluorescence - gene expression - temperature - tissue

Related subjects » Biochemistry & Biophysics

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