Overview
- Editors:
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Grant N. Pierce
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Institute of Cardiovascular Sciences, St. Boniface General Hospital Research Centre, Winnipeg, Canada
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William C. Claycomb
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Department of Biochemistry and Molecular Biology, Louisiana State University Medical Center, New Orleans, USA
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Table of contents (22 chapters)
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- Michael J. Mann, Ryuichi Morishita, Gary H. Gibbons, Heiko E. von der Leyen, Victor J. Dzau
Pages 3-12
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- Jeanette Hyer, Takashi Mikawa
Pages 23-35
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- Hiroaki Ooboshi, C. David RÃos, Donald D. Heistad
Pages 37-46
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- Jia-Li Gu, Jerry Nadler, John Rossi
Pages 47-57
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- Lorraine Yau, Peter Zahradka
Pages 59-66
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- R. Daniel Gietz, Anne Robbins, Kevin C. Graham, Barbara Triggs-Raine, Robin A. Woods
Pages 67-79
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- C. C. Liew, D. M. Hwang, R. X. Wang, S. H. Ng, A. Dempsey, D. H. Y. Wen et al.
Pages 81-87
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- R. Hullin, F. Asmus, G. Steinbeck
Pages 89-95
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- Michael P. Czubryt, Bram Ramjiawan, Grant N. Pierce
Pages 97-102
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- Manuela Bartoli, William C. Claycomb
Pages 103-109
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- Stephania Cormier-Regard, Daniel B. Egeland, Vivien J. Tannoch, William C. Claycomb
Pages 111-120
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- Alba Vecchini, Luciano Binaglia, Vincenzo Panagia
Pages 129-136
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- Gary D. Lopaschuk, Rick L. Barr
Pages 137-147
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- Yan-Jun Xu, Qiming Shao, Naranjan S. Dhalla
Pages 149-157
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- James S. C. Gilchrist, Chris Palahniuk, Ratna Bose
Pages 159-170
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- Ghassan Bkaily, Pierre Pothier, Pedro D’Orléans-Juste, May Simaan, Danielle Jacques, Doris Jaalouk et al.
Pages 171-194
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- Mahboubeh Eghbali-Webb, Augustine E. Agocha
Pages 195-198
About this book
Experimental techniques are the life blood of science. The better the methodology is, the more reliable and accurate the results will be. Ultimately, this will lead to a clearer interpretation of those results and firmer conclusions from any set of experiments. Experimental methodology in the area of cardiovascular biochemistry and molecular biology has advanced considerably in the last decade. Because of these factors, it was thought that a focused issue of Molecular and Cellular Biochemistry dedicated to the novel, latest technological advances in the field was warranted. We must thank Dr Naranjan S. Dhalla, Editor-in-Chief of Molecular and Cellular Biochemistry, for his willingness to publish an issue with such a focus. We have attracted some of the leaders in the field of cardiovascular biology to submit articles describing some of the most novel, significant techniques currently in use in their laboratories. The purpose of the manuscripts was not to describe the recent experimental findings from each laboratory as is done in most conventional manuscripts. Instead, the purpose of the articles found within this focused volume of Molecular and Cellular Biochemistry was to describe how the technique is performed on the laboratory bench so that others less familiar with the technique may be able to use it in their own labs. The subjects described in this volume can be generally subdivided into three categories: molecular biology, cell biology and basic biochemistry. The methods cover wide areas including various DNA and RNA expression technologies, transfection techniques, quantification of ion flux movement, measurements of lipid metabolism, advances in the culture of specific cardiovascular cell populations, and the use of confocal microscopy to examine cell structure and function. We thank all of the authors who have contributed so much of their time and efforts and, most importantly, shared the `secrets' of these valuable techniques with the rest of the cardiovascular research community.
Editors and Affiliations
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Institute of Cardiovascular Sciences, St. Boniface General Hospital Research Centre, Winnipeg, Canada
Grant N. Pierce
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Department of Biochemistry and Molecular Biology, Louisiana State University Medical Center, New Orleans, USA
William C. Claycomb
