Handbook of Biological Confocal Microscopy

1. Front Matter

   Pages i-xxviii

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2. Foundations of Confocal Scanned Imaging in Light Microscopy

   • Shinya Inoué

   Pages 1-19

3. Fundamental Limits in Confocal Microscopy

   • James B. Pawley

   Pages 20-42

4. Special Optical Elements

   • Jens Rietdorf, Ernst H. K. Stelzer

   Pages 43-58

5. Points, Pixels, and Gray Levels: Digitizing Image Data

   • James B. Pawley

   Pages 59-79

6. Laser Sources for Confocal Microscopy

   • Enrico Gratton, Martin J. vandeVen

   Pages 80-125

7. Non-Laser Light Sources for Three-Dimensional Microscopy

   • Andreas Nolte, James B. Pawley, Lutz Höring

   Pages 126-144

8. Objective Lenses for Confocal Microscopy

   • H. Ernst Keller

   Pages 145-161

9. The Contrast Formation in Optical Microscopy

   • Ping-Chin Cheng

   Pages 162-206

10. The Intermediate Optical System of Laser-Scanning Confocal Microscopes

    • Ernst H. K. Stelzer

    Pages 207-220

11. Disk-Scanning Confocal Microscopy

    • Derek Toomre, James B. Pawley

    Pages 221-238

12. Measuring the Real Point Spread Function of High Numerical Aperture Microscope Objective Lenses

    • Rimas Juškaitis

    Pages 239-250

13. Photon Detectors for Confocal Microscopy

    • Jonathan Art

    Pages 251-264

14. Structured Illumination Methods

    • Rainer Heintzmann

    Pages 265-279

15. Visualization Systems for Multi-Dimensional Microscopy Images

    • N. S. White

    Pages 280-315

16. Automated Three-Dimensional Image Analysis Methods for Confocal Microscopy

    • Badrinath Roysam, Gang Lin, Muhammad-Amri Abdul-Karim, Omar Al-Kofahi, Khalid Al-Kofahi, William Shain et al.

    Pages 316-337

17. Fluorophores for Confocal Microscopy: Photophysics and Photochemistry

    • Roger Y. Tsien, Lauren Ernst, Alan Waggoner

    Pages 338-352

18. Practical Considerations in the Selection and Application of Fluorescent Probes

    • Iain D. Johnson

    Pages 353-367

19. Guiding Principles of Specimen Preservation for Confocal Fluorescence Microscopy

    • Robert Bacallao, Sadaf Sohrab, Carrie Phillips

    Pages 368-380

20. Confocal Microscopy of Living Cells

    • Michael E. Dailey, Erik Manders, David R. Soll, Mark Terasaki

    Pages 381-403

Once the second edition was safely off to the printer, the 110 larger world of micro-CT and micro-MRI and the smaller world authors breathed a sigh of relief and relaxed, secure in the belief revealed by the scanning and transmission electron microscopes. that they would “never have to do that again. ” That lasted for 10 To round out the story we even have a chapter on what PowerPoint years. When we ?nally awoke, it seemed that a lot had happened. does to the results, and the annotated bibliography has been In particular, people were trying to use the Handbook as a text- updated and extended. book even though it lacked the practical chapters needed. There As with the previous editions, the editor enjoyed a tremendous had been tremendous progress in lasers and ?ber-optics and in our amount of good will and cooperation from the 124 authors understanding of the mechanisms underlying photobleaching and involved. Both I, and the light microscopy community in general, phototoxicity. It was time for a new book. I contacted “the usual owe them all a great debt of gratitude. On a more personal note, I suspects” and almost all agreed as long as the deadline was still a would like to thank Kathy Lyons and her associates at Springer for year away.

• Biological
• Confocal
• Microscopy
• Pawley
• chemistry
• databases
• electron microscopy
• fluorescence
• preparation

• Department of Zoology, University of Wisconsin, Madison, USA

  James B. Pawley

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