Genetic Preservation of Plant Cells in Vitro

1. Front Matter

   Pages I-XI

   PDF

2. Introduction to the in Vitro Preservation of Plant Cells, Tissues and Organs

   • B. W. W. Grout

   Pages 1-20

3. Minimal Growth Storage

   • B. W. W. Grout

   Pages 21-27

4. Cryopreservation of Protoplast, Suspension and Callus Cultures

   • B. W. W. Grout

   Pages 29-46

5. Cryopreservation of Excised Meristems, Shoot Tips and Somatic Embryos

   • B. W. W. Grout

   Pages 47-61

6. Storage of Free Pollen, Pollen Embryos and the Zygotic Embryos of Seed by Cryopreservation and Freeze Drying

   • B. W. W. Grout, A. V. Roberts

   Pages 63-74

7. Conservation of Algae

   • J. G. Day, M. R. McLellan

   Pages 75-98

8. Cryopreservation by Vitrification

   • L. E. Towill

   Pages 99-111

9. Biochemical and Molecular Methods for Assessing Damage, Recovery and Stability in Cryopreserved Plant Germplasm

   • K. Harding, E. E. Benson

   Pages 113-169

10. Back Matter

    Pages 171-171

    PDF

The long-term storage and maintenance of viable plant cells and organs is an area of active concern across the range of pure and applied plant sciences. In academic, government and commercial laboratories, the extended storage of propagules of one sort or another, with maximum protection of the genome from mutation and altered expression, is often a very necessary activity that can draw heavily on resources and effort. However, preservation per se is typically not an activity in its own right, but a facilitating technology that is part of a larger programme of work. Consequently, there are many laboratories that do not have the benefit of a specialist in storage technology, and have to delegate the responsibility to individuals, or teams, who are faced with a daunting learning curve. To maximise the chances of success, in the shortest possible time and with minimum losses, these researchers need sources of reference that are au­ thoritative and soundly based in practical experience.

• Algen
• Gefrierkonservierung
• Meristem
• Pflanzengewebe
• Pflanzenkultur
• Protoplasten
• cryopreservation
• plant
• plant breeding
• plant tissue
• plants
• protoplasts
• seeds
• tissue
• tissue culture

"Each chapter is written as a "hands-on" practical guide and presents procedures that can be adapted to individual laboratory conditions and modified to best suit the particular material being preserved." Bioscope

• Crisp Innovar Ltd., Reepham, Norfolk, UK

  Brian Grout

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