Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1022)
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Table of contents (31 protocols)
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Front Matter
Keywords
About this book
Glycosyltransferases (GTs) are essential for the biosynthesis of complex glycoconjugates and are powerful tools to study the functions of complex glycans in health, development and disease. Complex glycoconjugates, such as glycoproteins, proteoglycans and glycolipids, are assembled by GTs which synthesize specific linkages between sugars or sugars and protein. This is in contrast to the non-specific or less specific chemical glycation reactions, transglycosylation and reverse glycosylation reactions. Glycosyltransferases: Methods and Protocols contains a wide range of studies, methods and protocols which form a solid basis for investigations of the role and mechanisms, biology and pathology involving GTs. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
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Authoritative and easily accessible, Glycosyltransferases: Methods and Protocols is a vital contribution to glycobiology and glycopathology, and to applications of these enzymes in biotechnology and drug development. It will prove invaluable to students, postdoctoral fellows, and senior scientists carrying on research of GTs that has been intensified over the last years.
Editors and Affiliations
Bibliographic Information
Book Title: Glycosyltransferases
Book Subtitle: Methods and Protocols
Editors: Inka Brockhausen
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-465-4
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2013
Hardcover ISBN: 978-1-62703-464-7Published: 14 June 2013
Softcover ISBN: 978-1-4939-5996-9Published: 23 August 2016
eBook ISBN: 978-1-62703-465-4Published: 14 June 2013
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XVII, 425
Number of Illustrations: 70 b/w illustrations, 40 illustrations in colour
Topics: Biochemistry, general, Analytical Chemistry, Carbohydrate Chemistry