Overview
- Presents current up-to-date methodologies
- Features tips on trouble shooting and avoiding known pitfalls
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1020)
Access this book
Tax calculation will be finalised at checkout
Other ways to access
Table of contents(16 protocols)
About this book
Recently, many ground-breaking steps have been made towards better understanding NO/cGMP/PKG pathways, its components, substrates, and their localization within a given cell. These advances were only possible due to the development of sophisticated new techniques in the field. In Guanylate Cyclase and Cyclic GMP: Methods and Protocols expert researchers in the field seek to provide an overview of novel techniques to identify various elements of the NO/cGMP/PKG pathway and further characterize their function, signaling, localization, and importance on a cellular level and in whole animal models providing a higher patho-/physiological integration and relevance. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Guanylate Cyclase and Cyclic GMP: Methods and Protocols seeks to provide scientist current methods and a useful guide towards the possibility to apply these techniques to their own research.
Editors and Affiliations
-
Addenbrooke’s Hospital, Department of Medicine, University of Cambridge, Cambridge, United Kingdom
Thomas Krieg
-
Toxicology and Clinical Pharmacy, Department of Pharmacology, Universität Tübingen, Tübingen, Germany
Robert Lukowski
Bibliographic Information
Book Title: Guanylate Cyclase and Cyclic GMP
Book Subtitle: Methods and Protocols
Editors: Thomas Krieg, Robert Lukowski
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-459-3
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2013
Hardcover ISBN: 978-1-62703-458-6
Softcover ISBN: 978-1-4939-6320-1
eBook ISBN: 978-1-62703-459-3
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 249
Number of Illustrations: 21 b/w illustrations, 25 illustrations in colour
Topics: Biochemistry, general