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Microarrays

Volume I: Synthesis Methods

Editors:

  • Meticulous exploration into state-of-the-art methods and protocols for microarray technology
  • Authors examine bioinformatics, an important aspect in analyzing large amounts of data that emerge during experiments

Part of the book series: Methods in Molecular Biology (MIMB, volume 381)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xix
  2. Introduction

    • Hartmut Seliger
    Pages 1-36
  3. Current Microarray Surface Chemistries

    • David W. Grainger, Charles H. Greef, Ping Gong, Michael J. Lochhead
    Pages 37-57
  4. Nonfouling Surfaces

    • Ping Gong, David W. Grainger
    Pages 59-92
  5. Optimization of Oligonucleotide DNA Microarrays

    • Martin Dufva, Claus B. V. Christensen
    Pages 93-103
  6. Evaluating the Quality of Data From Microarray Measurements

    • Lili. Wang, A. K. Gaigalas, M. B. Satterfield, M. Salit, Y. Zong, J. Noble
    Pages 121-131
  7. Construction of Oligonucleotide Microarrays (Biochip) Using Heterobifunctional Reagents

    • Jyoti Choithani, B. Vaijayanthi, Pradeep Kumar, Kailash Chand Gupta
    Pages 133-163
  8. Choice of Polymer Matrix, Its Functionalization and Estimation of Functional Group Density for Preparation of Biochips

    • Shweta Mahajan, Bhashyam Vaijayanthi, Gopal Rembhotkar, Kailash Chand Gupta, Pradeep Kumar
    Pages 165-187
  9. Methods in High-Resolution, Array-Based Comparative Genomic Hybridization

    • Mark R. McCormick, Rebecca R. Selzer, Todd A. Richmond
    Pages 189-211
  10. Construction of In Situ Oligonucleotide Arrays on Plastic

    • Jang B. Rampal, Peter J. Coassin, Robert S. Matson
    Pages 227-246
  11. Detecting Ligated Fragments on Oligonucleotide Microarrays

    • Ian R. Berry, Carol A. Delaney, Graham R. Taylor
    Pages 247-265
  12. Detection of Single-Nucleotide Polymorphisms in Cancer-Related Genes by Minisequencing on a Microelectronic DNA Chip

    • Alexandre Ho-Pun-Cheung, Hafid Abaibou, Philippe Cleuziat, Evelyne Lopez-Crapez
    Pages 267-278
  13. Hybridization Analysis Using Oligonucleotide Probe Arrays

    • Robert S. Matson, Jang B. Rampal
    Pages 279-298
  14. In Situ Synthesis of Peptide Microarrays Using Ink-Jet Microdispensing

    • Bogdan V. Antohe, Patrick W. Cooley
    Pages 299-312
  15. Intein-Mediated Peptide Arrays for Epitope Mapping and Kinase/Phosphatase Assays

    • Ming-Qun Xu, Inca Ghosh, Samvel Kochinyan, Luo Sun
    Pages 313-338
  16. Printing Low Density Protein Arrays in Microplates

    • Robert S. Matson, Raymond C. Milton, Michael C. Cress, Tom S. Chan, Jang B. Rampal
    Pages 339-361
  17. Forward-Phase and Reverse-Phase Protein Microarray

    • Yaping Zong, Shanshan Zhang, Huang-Tsu Chen, Yunfei Zong, Yaxian Shi
    Pages 363-373
  18. Cell Microarray for Functional Exploration of Genomes

    • David Castel, Marie-Anne Debily, Amandine Pitaval, Xavier Gidrol
    Pages 375-384

About this book

Microarray Technology, Volumes 1 and 2, present information in designing and fabricating arrays and binding studies with biological analytes while providing the reader with a broad description of microarray technology tools and their potential applications. The first volume deals with methods and protocols for the preparation of microarrays. The second volume details applications and data analysis, which is important in analyzing the enormous data coming out of microarray experiments.

Among the topics discussed in Volume 1: Synthesis Methods, are matrices in the synthesis of microarrays, array optimization processes, array-based comparative genomic hybridization, 60-mer oligonucleotide probes, bifunctional reagents NTMTA and NTPAC, and high density arrays using digital microarray synthesis platforms. Other topics include multiplex ligation-dependent probe amplification (MLPA), hybridization conditions in situ-synthesized oligo arrays, peptide arrays, high density replication tools (HDRT), protocols for the quantification of oligo hybridization, glyco-bead arrays, and an investigation into the emerging nano technology.

Microarray Technology, Volumes 1 and 2, provide ample information to all levels of scientists from novice to those intimately familiar with array technology.

Editors and Affiliations

  • Beckman Coulter, Inc., Brea

    Jang B. Rampal

Bibliographic Information

  • Book Title: Microarrays

  • Book Subtitle: Volume I: Synthesis Methods

  • Editors: Jang B. Rampal

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-59745-303-5

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2007

  • Hardcover ISBN: 978-1-58829-589-7Published: 27 July 2007

  • Softcover ISBN: 978-1-61737-663-4Published: 19 November 2010

  • eBook ISBN: 978-1-59745-303-5Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XX, 452

  • Number of Illustrations: 114 b/w illustrations

  • Additional Information: Originally published by Academic Press, 2000

  • Topics: Biochemistry, general, Biotechnology, Human Genetics, Cell Biology, Molecular Medicine

Buy it now

Buying options

eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access