Animal Cell Technology: Basic & Applied Aspects

1. Front Matter

   Pages i-xiii

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2. The Value of Models in Animal Cell Biotechnology

   1. The Value of Models in Animal Cell Biotechnology

      • R. E. Spier

      Pages 1-5

3. The Manzanar Project — A Unique Way to End World Hunger

   1. The Manzanar Project — A Unique Way to End World Hunger

      • Gordon H. Sato

      Pages 7-8

4. Cell Characterization and Validation

   1. Experience in Cell Line Testing

      • W. Alan Moore

      Pages 9-14

   2. Virus Removal and Inactivation in Process Validation

      • Sei-ichi Manabe

      Pages 15-30

   3. Multilocus DNA Fingerprinting in the Analysis of Cell Stocks: Stability Studies and Application to a Wide Range of Species

      • Glyn N. Stacey, Bryan J. Bolton, Alan Doyle

      Pages 31-39

   4. Alteration of Cell Surface Glycoproteins through Differentiation from a Human Histiocytic Lymphoma Cell Line, U-937, to a Macrophage-Like Cell Line, U-M

      • K. Hata, M. Kobori, Z-L. Kong, K. Shinohara

      Pages 41-47

   5. Removability of Virus Particles and Permeability of Protein from Cell Culture Medium Using Cuprammonium Regenerated Cellulose Hollow Fiber (BMM™)

      • T. Hirasaki, T. Tsuboi, T. Noda, S. Uematsu, G. Ishikawa, A. Kono et al.

      Pages 49-55

5. Animal Cell Bioprocess Engineering

   1. An Evaluation of Hybridoma Cell-Specific Productivity: Perfused Immobilized, Continuous Suspension, and Batch Suspension Cultures

      • P. W. Runstadler, S. S. Ozturk, N. G. Ray

      Pages 57-63

   2. Dynamics of Aggregate Culture of Mammalian Cells

      • Yun-Seung Kyung, Madhusudan V. Peshwa, Cheryl M. Perusich, Wei-Shou Hu

      Pages 65-69

   3. Effect of Protein Adsorption on Cell Attachment on Solid Surfaces

      • Motoyuki Suzuki, Yasuyuki Sakai

      Pages 71-76

   4. Characterization of Cell Growth and Improvement of Attachment Kinetics on Macroporous Microcarriers

      • Jung-Hoe Kim, Hun-Soo Lim, Bong-Kwan Han, Madhusudan V. Peshwa, Wei-Shou Hu

      Pages 77-80

   5. Application of Three Dimensional Culture of Adult Rat Hepatocytes in PUF Pores for Artificial Liver Support System

      • H. Ijima, Y. Taniguchi, T. Matsushita, K. Funatsu

      Pages 81-86

   6. Novel Validation Method of Virus-Removability for Biological Cell Culture Products Using Polymeric Membrane Filters

      • H. Nakano, S. Manabe, S. Uematsu, T. Sato, N. Osawa, T. Hirasaki et al.

      Pages 87-102

   7. Selective Formation and Immobilization by Re-Attachment of Floating Spheroids of Rat Hepatocytes

      • Yasuyuki Sakai, Motoyuki Suzuki

      Pages 103-108

   8. Engineering of Ultrafiltration Hollow Fiber Bioreactors for Mammalian Cell Protein Production

      • A. Y. Patkar, D. G. Taylor, B. D. Bowen, J. M. Piret

      Pages 109-114

   9. Production of hEPO by Recombinant C127 Cells using Hollow Fiber Culture System

      • Hiroshi Takasugi, Noboru Fujikawa, Shin Shimizu

      Pages 115-120

   10. Oscillatory Behavior in Continuous Aggregate Cultures of Mammalian Cells

       • H. Vits, M. V. Peshwa, W.-S. Hu

       Pages 121-127

   11. Detection of Mammalian Cell Attachment and Lysis on a Metal Surface Using a Piezoelectric Sensor

       • David M. Gryte, Madhusudan V. Peshwa, Wei-Shou Hu, Michael D. Ward

       Pages 129-134

6. High Density Culture and Cell Culture System

   1. Strategies for Economic GMP Production of Mammalian Cell Expressed Proteins

      • Frieder K. Hofmann

      Pages 135-141

New data on animal cell technology are brought together in this volume, with emphasis given to the basic characterization of cell lines. The merits of different cell culture systems are examined and investigations into the factors influencing cell growth and productivity are presented. A special section deals with the biological properties of proteins produced by engineered animal cells. All those involved in the culture of animal cells will find this volume invaluable.

• bioprocess engineering
• cell
• cell culture
• protein
• proteins
• system

• Graduate School of Genetic Resources Technology, Kyushu University, Japan

  H. Murakami, S. Shirahata, H. Tachibana

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