Animal Cell Technology: Basic & Applied Aspects

1. Front Matter

   Pages i-xvii

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2. Search for Edible Plant Constituents of Anti-Tumor Promotion Using an Inhibition Test of Epstein-Barr Virus Activation in Raji Cells

   1. Search for Edible Plant Constituents of Anti-Tumor Promotion Using an Inhibition Test of Epstein-Barr Virus Activation in Raji Cells

      • K. Koshimizu

      Pages 1-8

3. Cell Characterization

   1. The Nature and Effect of Cell-Cell Interactions between Recombinant CHO Cells Producing Human Interferon-Gamma.

      • S. R. Coppen, R. Newsam, A. J. Baines, A. T. Bull, C. Caulcott

      Pages 9-18

   2. Characterization of Newly Established Cells Which Provide an Animal Model for Spontaneous Metastasis

      • Takahiro Nomura, Kazuo Ryoyama, Gensaku Okada, Sadaya Matano, Haruhiko Tokuyama, Isao Hori et al.

      Pages 19-24

   3. Bovine Mammary Epithelial Cells: Morphology, Protein Synthesis and Gene Transfection

      • T. Matsuda, J. Ahn, Y. Mizuno, T. Adachi, N. Aoki, R. Nakamura

      Pages 25-31

   4. Growth and Differentiation of a Small Intestinal Epithelial Cell Line IEC-6

      • Akio Ametani, Satoshi Hachimura, Akemi Imaoka, Yoshio Yamamoto, Ho-Keun Yi, Shuichi Kaminogawa

      Pages 33-40

   5. Subunit Assembly of Laminin Variants in Cultured Animal Cells

      • Hoon Jeon, Masaaki Ono, Chino Kumagai, Yasuo Kitagawa

      Pages 41-48

   6. Regulation of Mitochondrial Gene Expression by Interferons

      • Hidetoshi Inagaki, Yuichi Matsushima, Mikiko Ohshima, Yasuo Kitagawa

      Pages 49-55

4. Expression of Recombinant Animal Genes in Cultured Cells

   1. Production of the Human Growth Hormone in Serum-Free UC203 Medium

      • Masahiro Mizuguchi, Ken Matsumoto, Kazukiyo Onodera

      Pages 57-61

   2. Stable Production of Pro-urokinase and its Derivative by Namalwa KJM-1 Cells Adapted to a Serum-free Medium

      • Shinji Hosoi, Mitsuo Satoh, Hiromasa Miyaji, Tatsunari Nishi, Tamio Mizukami, Mamoru Hasegawa et al.

      Pages 63-70

   3. Production and Characterization of Recombinant Soluble form Erythropoietin Receptor

      • Masaya Nagao, Seiji Masuda, Satoshi Abe, Masatsugu Ueda, Ryuzo Sasaki

      Pages 71-77

   4. A ts-SV40 Based New Mammalian Plasmid Vector and Its Copy Number Control by Temperature Change

      • Hideyo Kirinaka, Shinji Iijima, Takeshi Kobayashi

      Pages 79-84

   5. Selection of Regulatory Mutants of μ Gene Expression from Myeloma Cells Transfected with the Modified μ Gene

      • T. Shindo, H. Ueda, F. Makishima, E. Suzuki, H. Nishimura

      Pages 85-91

   6. Process Control of BHK Cells by Nucleotide Pool Measurement

      • Ulrich Valley, Thomas Ryll, Roland Wagner

      Pages 93-97

   7. Purification and cDNA Cloning of Flatfish Interferon

      • T. Tamai, T. Noguchi, N. Sato, S. Kimura, S. Shirahata, H. Murakami

      Pages 99-105

   8. Detection by Fluorescence in Situ Hybridization of the Erythropoietin Gene Introduced into Animal Cells

      • K. Okumura, Y. Horikita, K. Hishida, H. Taguchi, Y. Shimabayashi, M. Nagao et al.

      Pages 107-113

   9. High-Level Expression of Recombinant Bovine ß-Lactoglobulin in Insect Cells

      • K. Mizumachi, J. Kurisaki, N. M. Tsuji

      Pages 115-121

   10. Mass Production and Purification of Recombinant Flatfish Interferon

       • T. Tamai, T. Noguchi, K. Tsujimura, N. Sato, S. Kimura, S. Shirahata et al.

       Pages 123-127

5. Cell Culture for Assessment of Physiologically Functional and Hazardous Substances

   1. Stimulation of Protein Degradation By Tumor Necrosis Factor a in Cultured Muscle Cells

      • K. Yagasaki, Y. Sakazaki, Y. Kida, Y. Miura, R. Funabiki

      Pages 129-133

   2. From in Vitro Cell Culture to in Vivo Animal Study: Demonstration that Epidermal Growth Factor (EGF) Is a Potent Inhibitor of Adipose Differentiation.

      • Ginette Serrero

      Pages 135-141

Animal cell technology has been making tremendous progress. Originally this term reminded people of engineering for high density and large volume culture of animal cells. At present many fields of biological sciences are aiming at advance in animal cell technology. Cell culture engineering is aided not only with developments in apparatus, matrix, media, and computational analysis, but also with new biological procedures in gene and protein technology, cell biological resources and immunological methods. Results obtained with animal cell technology are applied to production of pharmaceuticals, diagnosis reagents and food endowed with physiological functions, and cell and gene therapy of animals and humans, and useful for elucidating scientific phenomena. It is also essential to establish methods of evaluation for functionality and safety of newly discovered molecules and cells. The progress in animal cell technology is supported by, and attributes in both of basic and applied sciences. The proceedings of the Fifth International Meeting of the Japanese Association for Animal Cell Technology (JAACT) covers the subjects above mentioned. The articles in this book will help researchers in many fields to understand the current status and future trends in animal cell technology. JAACT organized this Meeting and we express our gratitude to the members of JAACT. We gratefully acknowledge all the members of the organizing committee for their dedication in assuring the Meeting's success. For their valuable supports, we also thank the Japanese Biolndustry Association and Saitama Foundation for Culture and Industry.

• Activation
• Expression
• Monoclonal Antibodies
• gene expression
• microorganism
• protein

• Department of Agricultural Chemistry, The University of Tokyo, Japan

  S. Kaminogawa, A. Ametani, S. Hachimura

Policies and ethics