Editors:
- Provides step-by-step instructions and helpful troubleshooting notes
- Published in cooperation with the International Society for Transgenic Technology (ISTT)
- With contributions by leading scientists
- Includes supplementary material: sn.pub/extras
Part of the book series: Springer Protocols Handbooks (SPH)
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Table of contents (26 protocols)
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Front Matter
About this book
This laboratory manual, published in cooperation with the International Society for Transgenic Technology (ISTT), provides almost all current methods that can be applied to the creation and analysis of genetically modified animals. The chapters have been contributed by leading scientists who are actively using the technology in their laboratories. Based on their first-hand experience the authors also provide helpful notes and troubleshooting sections. Topics range from standard techniques, such as pronuclear microinjection of DNA, to more sophisticated and modern methods, such as the derivation and establishment of embryonic stem (ES) cell lines, with defined inhibitors in cell culture medium. In addition, related topics with relevance to the field are addressed, including global web-based resources, legal issues, colony management, shipment of mice and embryos, and the three R’s: refinement, reduction and replacement.
Editors and Affiliations
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Div. Biology, California Institute of Technology, Pasadena, USA
Shirley Pease
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Transgenic Animal Model Core, University of Michigan, Ann Arbor, USA
Thomas L. Saunders
Bibliographic Information
Book Title: Advanced Protocols for Animal Transgenesis
Book Subtitle: An ISTT Manual
Editors: Shirley Pease, Thomas L. Saunders
Series Title: Springer Protocols Handbooks
DOI: https://doi.org/10.1007/978-3-642-20792-1
Publisher: Springer Berlin, Heidelberg
eBook Packages: Springer Protocols
Copyright Information: Springer-Verlag Berlin Heidelberg 2011
Hardcover ISBN: 978-3-642-20791-4Published: 31 August 2011
Softcover ISBN: 978-3-662-51943-1Published: 23 August 2016
eBook ISBN: 978-3-642-20792-1Published: 30 August 2011
Series ISSN: 1949-2448
Series E-ISSN: 1949-2456
Edition Number: 1
Number of Pages: XV, 669
Number of Illustrations: 145 b/w illustrations, 65 illustrations in colour
Topics: Animal Genetics and Genomics, Transgenics