Animal Cell Technology: Basic & Applied Aspects

1. Front Matter

   Pages i-xi

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2. Establishing new cell lines and characterizing molecular biological properties of cultured cells

   1. Stress-Inducible Gene Expression in Fish Cells

      • M. Yamashita

      Pages 1-4

   2. Cell Culture and Some Properties of Fish Immunocytes

      • Tasuku Watanabe, Tadaaki Moritomo

      Pages 5-9

   3. Telomerase regulation in differentiated serum-free mouse embryo cells

      • Y. Katakura, K. Yamamoto, O. Miyake, E. Nakata, T. Yasuda, N. Uehara et al.

      Pages 11-15

   4. Immortalization of Mammalian Central Nervous System Stem Cells From p53 Knock-out Forebrain

      • T. Hisatsune, K. Yamada, R. D. G. McKay, S. Kaminogawa

      Pages 17-22

   5. Neuroprotective Function of Erythropoietin in the Central Nervous System

      • Seiji Masuda, Mariko Chikuma, Emi Morishita, Masaya Nagao, Ryuzo Sasaki

      Pages 23-27

   6. Selection and Stability for a Recombinant CHO Cell Line Expressing Human GM-CSF in Gene Amplification

      • Takeshi Omasa, Seima Itami, Daisuke Kameoka, Yoshio Katakura, Ken-ichi Suga

      Pages 29-33

   7. Effect of Culture Conditions on Glycosylation in Hybridoma Antibody Production

      • Takeshi Omasa, Jyun-ya Tanaka, Kenji Amimoto, Yoshio Katakura, Ken-ichi Suga

      Pages 35-39

   8. Antisense Rb RNA Inhibits in Vitro Myogenic Differentiation in a Myoblast Cell Line, C2

      • Masayuki Kobayashi, Yukika Yamauchi, Akiko Tanaka

      Pages 41-44

   9. Establishment of a Stable hGH Producing Verots S3 Transformant

      • Ayuko Sato, Nakao Nomura, Kaoru Saijo, Tadao Ohno, Masatoshi Matsumura

      Pages 45-49

   10. Characterization of an Antigen-Producing Cell Line Hugk-14 at the Molecular Level

       • Noboru Nakamichi, Asao Noda, Tomonori Yonezu, Katsuro Koike, Toshiharu Matsumura

       Pages 51-57

   11. VJ Rearrangement at Previously Excluded Igλ Allele in Human Plasma Cell Line NAT-30

       • Hirotaka Haruta, Sanetaka Shirahata, Hirofumi Tachibana

       Pages 59-63

3. Effect of various substances on animal cell response

   1. Rational Drug Design Using Mammalian Cell Lines Expressing Site-Directed Mutants of the Human H1 Histaminic Receptor

      • N. Moguilevsky, E. Differding, M. Gillard, A. Bollen

      Pages 65-69

   2. Biologic Response of Endothelial Cells: Modulation of Adhesion and Multiplication by Cytokines and Prospects for the Production of a Vaccine Against the Rickettsia Cowdria ruminantium

      • T. Marique, D. Blankaert, A. Raschella, V. Hendrick, B. Declerck, I. Teixera-Guerra et al.

      Pages 71-75

   3. Effects of Chemicals on the Immune Response

      • Akio Ametani, Hiroko Watanabe, Satoshi Hachimura, Kwang-Young Lee, Shuichi Kaminogawa

      Pages 77-81

   4. Molecular Mechanisms of Selaginella Tamariscina for Antineoplastic Activity in Human Leukemia Cell Line U937

      • In Seon Lee, Soo Un Kim, Yun Sik Lee, Sung Hui Park, In Ja Rhee

      Pages 83-89

   5. Differential Effect of Antigen Analogs with T cell Inhibitory Activity on in vivo Antibody Response

      • Masako Toda, Mamoru Totsuka, Tohru Yoshioka, Akio Ametani, Shuichi Kaminogawa

      Pages 91-95

   6. Increase of Intracellular Ca2+ Level Induces Osteoclast- Like Cell Formation in Coculture of Mouse Bone Marrow Cells and Osteoblastic Cells

      • Masamichi Takami, Je-Tae Woo, Kazuo Nagai

      Pages 97-101

   7. New Angiogenesis Inhibitors of Microbial Origin

      • T. Shibata, T. Otsuka, H. Terano, M. Okuhara

      Pages 103-107

   8. Hydrocortisone Induces a Formation of Basophilic Granules in the Human Leukemia Cell Line KU812

      • Takashi Hara, Sanetaka Shirahata, Hirofumi Tachibana

      Pages 109-113

Animal cell technology is a growing discipline of cell biology which aims not only to understand structures, functions and behaviors of differentiated animal cells but also to uncover their abilities for industrial and medical purposes. The goal of animal cell technology includes clonal expansion of differentiated cells with useful abilities, optimization of their culture conditions on the industrial scale, modulation of their ability in order efficiently to produce medically and pharmaceutically important proteins, and application of animal cells to gene therapy and formation of artificial organs. This Volume gives the readers a complete review of the present state of the art in Japan, a country where this field is well advanced, as well as in Asia, Europe and the United States. The Proceedings will be useful for cell biologists, biochemists, molecular biologists, biochemical engineers and those in other disciplines related to animal cell culture, working in academic environments as well as in the biotechnology and pharmaceutical industries.

• biotechnology
• cell biology
• development
• gene therapy
• protein
• proteins

• Department of Bioengineering, Tokyo Institute of Technology, Yokohama, Japan

  K. Nagai, M. Wachi

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