Overview
- Editors:
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John Kuo
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Centre for Microscopy and Microanalysis, The University of Western Australia, Crawley, Australia
- Essential electron microscopy techniques for TEM and SEM
- Cutting-edge cryo-EM and 3D TEM techniques
- Quantitative aspects of immunogold labeling in resin embedded samples
- Examines the application of electron energy-loss spectroscopy (EELS) to biomedical tissue
- Notes section enables efficient adaptation and troubleshooting of protocols
- Includes supplementary material: sn.pub/extras
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Table of contents (28 protocols)
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- José A. Mascorro, John J. Bozzola
Pages 19-34
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- Kent L. McDonald, Mary Morphew, Paul Verkade, Thomas Müller-Reichert
Pages 143-173
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- Dimitri Vanhecke, Luca Studer, Daniel Studer
Pages 175-197
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- Margaret Coughlin, William M. Brieher, Ryoma Ohi
Pages 199-212
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- Dušan Cmarko, Karel Koberna
Pages 213-228
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- Heinz Schwarz, Bruno M. Humbel
Pages 229-256
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- Paul Webster, Alexandre Webster
Pages 257-289
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- Paul A. Sims, Jeff D. Hardin
Pages 291-308
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- Hui-Ting Chou, James E. Evans, Henning Stahlberg
Pages 331-343
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- Peter Engelhardt, Jari Meriläinen, Fang Zhao, Susumu Uchiyama, Kiichi Fukui, Veli-Pekka Lehto
Pages 387-405
About this book
In this revised and expanded second edition, Electron Microscopy: Methods and Protocols presents the newest technology in electron microscopy, while maintaining the practicality and accessibility of the acclaimed first edition. Like the first edition, this volume provides clear, concise instructions on processing biological specimens and includes discussion on the underlying principles of the majority of the processes presented. Electron Microscopy comprises two major areas of electron microscopy-transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The TEM area covers several key techniques, including: conventional specimen preparation methods for cultured cells and biomedical and plant tissues; cryospecimen preparation by high-pressure freezing and cryoultramicrotomy negative staining and immunogold labeling techniques; and TEM crystallography and cryo-TEM tomography. The SEM area similarly attends to conventional-, variable pressure-, environmental-, and cryoscanning microscopy techniques, as well as the application of X-ray microanalysis. Protocols for the application of X-ray microanalysis to SEM and mass spectrometry conclude the volume.
Reviews
"This is an excellent book, with well-written chapters. The protocols are easy to follow and well organized. This book is highly recommended for beginners to advanced level microscopists." -Micron
Editors and Affiliations
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Centre for Microscopy and Microanalysis, The University of Western Australia, Crawley, Australia
John Kuo
