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Biological Low-Voltage Scanning Electron Microscopy

  • Book
  • © 2008

Overview

Editors:
  1. Heide Schatten

    1. Department of Veterinary Pathobiology, University of Missouri-Columbia, Columbia, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. James B. Pawley

    1. Department of Zoology, University of Wisconsin, Madison, USA

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    You can also search for this editor in PubMed   Google Scholar

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Table of contents (11 chapters)

  1. Front Matter

    Pages I-X
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  2. The Early Development of the Scanning Electron Microscope

    • Dennis McMullan
    Pages 1-25

  3. LVSEM for Biology

    • James B. Pawley
    Pages 27-106

  4. The Aberration-Corrected SEM

    • David C. Joy
    Pages 107-127

  5. Noise and Its Effects on the Low-Voltage SEM

    • David C. Joy
    Pages 129-144

  6. High-Resolution, Low Voltage, Field-Emission Scanning Electron Microscopy (HRLVFESEM) Applications for Cell Biology and Specimen Preparation Protocols

    • Heide Schatten
    Pages 145-169

  7. Molecular Labeling for Correlative Microscopy: LM, LVSEM, TEM, EF-TEM and HVEM

    • Ralph Albrecht, Daryl Meyer
    Pages 171-196

  8. Low kV and Video-Rate, Beam-Tilt Stereo for Viewing Live-Time Experiments in the SEM

    • Alan Boyde
    Pages 197-214

  9. Cryo-SEM of Chemically Fixed Animal Cells

    • Stanley L. Erlandsen
    Pages 215-215

  10. High-Resolution and Low-Voltage SEM of Plant Cells

    • Guy Cox, Peter Vesk, Teresa Dibbayawan, Tobias I. Baskin, Maret Vesk
    Pages 229-244

  11. High-Resolution Cryoscanning Electron Microscopy of Biological Samples

    • Paul Walther
    Pages 245-261

  12. Developments in Instrumentation for Microanalysis in Low-Voltage Scanning Electron Microscopy

    • Dale E. Newbury
    Pages 263-303

  13. Back Matter

    Pages 305-317
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Keywords

About this book

Major improvements in instrumentation and specimen preparation have brought SEM to the fore as a biological imaging technique. In FESEM, a field-emission cathode placed in the electron gun of a scanning electron microscope provides narrower probing beams and high electron energy. The result is improved spatial resolution and minimized sample charging and damage. Images produced are less destroyed and have a spatial resolution down to 1.5 nm, three to six times better than conventional SEM.

Although this imaging technique has undergone tremendous developments, it is still poorly represented in the literature, limited to journal articles and chapter s in books. This comprehensive volume is dedicated to the theory and practical applications of FESEM in biological samples. It provides a comprehensive explanation of instrumentation, applications, and protocols, and is intended to teach the reader how to operate such microscopes to obtain the best quality images.

Reviews

From the reviews:

"Topics ranging from the early development of the scanning electron microscope (SEM) to some of the latest low-voltage and cryo techniques are reviewed in detail. … this is a very useful volume for those wishing to learn, or refresh their memory, in the context of getting the best out of an SEM, and in particular the field emission SEM (FESEM)." (Iolo Ap Gwynn, infocus Magazine, Issue 12, December, 2008)

“Biological Low-voltage Scanning Electron Microscopy is an excellent and timely collection, written by many of the recognized experts in the subject … . Strongly recommended.” (Ultramicroscopy, Vol. 109, June, 2009)

Editors and Affiliations

  • Department of Veterinary Pathobiology, University of Missouri-Columbia, Columbia, USA

    Heide Schatten

  • Department of Zoology, University of Wisconsin, Madison, USA

    James B. Pawley

Bibliographic Information

  • Book Title: Biological Low-Voltage Scanning Electron Microscopy

  • Editors: Heide Schatten, James B. Pawley

  • DOI: https://doi.org/10.1007/978-0-387-72972-5

  • Publisher: Springer New York, NY

  • eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)

  • Copyright Information: Springer-Verlag New York 2008

  • Hardcover ISBN: 978-0-387-72970-1Published: 18 December 2007

  • Softcover ISBN: 978-1-4899-9584-1Published: 13 September 2014

  • eBook ISBN: 978-0-387-72972-5Published: 03 December 2007

  • Edition Number: 1

  • Number of Pages: X, 317

  • Topics: Biological Microscopy, Zoology, Cell Biology, Microbiology

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