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Plant Developmental Biology

Methods and Protocols

  • Book
  • © 2010

Overview

Editors:
  1. Lars Hennig

    1. (ETH), Department of Biology, Swiss Federal Institute of Technology, Zurich, Switzerland

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    You can also search for this editor in PubMed   Google Scholar

  2. Claudia Köhler

    1. (ETH), Department of Biology, Swiss Federal Institute of Technology, Zurich, Switzerland

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Provides instructions for handling of different plant model systems
  • Offers cutting-edge methods for analyzing plant development on the phenotypic, molecular and biochemical level
  • Includes in-depth explanations and troubleshooting advice for all methods
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 655)

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Table of contents (29 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Growth Protocols for Model Plants in Developmental Biology

    • Lars Hennig
    Pages 1-10

  3. Grafting as a Research Tool

    • Colin G.N. Turnbull
    Pages 11-26

  4. Virus-Induced Gene Silencing as a Reverse Genetics Tool to Study Gene Function

    • Steven Bernacki, Mansour Karimi, Pierre Hilson, Niki Robertson
    Pages 27-45

  5. The CRE/lox System as a Tool for Developmental Studies at the Cell and Tissue Level

    • Guy Wachsman, Renze Heidstra
    Pages 47-64

  6. Inducible Gene Expression Systems for Plants

    • Lorenzo Borghi
    Pages 65-75

  7. Trichome Development in Arabidopsis

    • Joachim F. Uhrig, Martin Hülskamp
    Pages 77-88

  8. Phenotyping the Development of Leaf Area in Arabidopsis thaliana

    • Sarah J. Cookson, Olivier Turc, Catherine Massonnet, Christine Granier
    Pages 89-103

  9. Analyzing Shoot Apical Meristem Development

    • Cristel C. Carles, Chan Man Ha, Ji Hyung Jun, Elisa Fiume, Jennifer C. Fletcher
    Pages 105-129

  10. Analyzing Floral Meristem Development

    • Elisa Fiume, Helena R. Pires, Jin Sun Kim, Jennifer C. Fletcher
    Pages 131-142

  11. Female Gametophytic Mutants: Diagnosis and Characterization

    • Ronny Völz, Rita Groß-Hardt
    Pages 143-153

  12. Pollen Tube Development

    • Mark A. Johnson, Benedikt Kost
    Pages 155-176

  13. Analysis of Root Meristem Size Development

    • Serena Perilli, Sabrina Sabatini
    Pages 177-187

  14. Phenotypic Characterization of Photomorphogenic Responses During Plant Development

    • Thomas Kretsch
    Pages 189-202

  15. Kinematic Analysis of Cell Division and Expansion

    • Bart Rymen, Frederik Coppens, Stijn Dhondt, Fabio Fiorani, Gerrit T.S. Beemster
    Pages 203-227

  16. Flowering Time Control

    • Yvonne Möller-Steinbach, Cristina Alexandre, Lars Hennig
    Pages 229-237

  17. mRNA Detection by Whole Mount In Situ Hybridization (WISH) or Sectioned Tissue In Situ Hybridization (SISH) in Arabidopsis

    • Yvonne Stahl, Rüdiger Simon
    Pages 239-251

  18. Immunolocalization of Proteins in Plants

    • Michael Sauer, Jiří Friml
    Pages 253-263

  19. Detection of Small Non-coding RNAs

    • Tamas Dalmay
    Pages 265-274

  20. Quantitative Real Time PCR in Plant Developmental Biology

    • Vivien Exner
    Pages 275-291
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Keywords

About this book

Plants come in myriads of shapes and colors, and the beauty of plants has fascinated mankind for thousands of years. Long before Mendel discovered the laws of heritab- ity and Darwin developed his theory on evolution, the affection for ornamental plants led people to select alleles that establish novel plant forms. Today, plant developmental biology tries to discover the mechanisms that control the establishment of specialized cell types, tissues, and organs from the fertilized egg during a plant’s life. Although the underlying processes of cell proliferation and differentiation are similar in plants and a- mals, plants are different because their development is usually open, and its outcome is not the faithful repetition of a general plan but is strongly in?uenced by environm- tal conditions. In the last few decades, plant developmental biology has pinpointed a large number of developmental regulators and their interactions and the mechanisms that govern plant development start to emerge. In part, this progress was enabled by the advance of powerful molecular tools for a few model species, most importantly Arabidopsis. This volume of the Methods in Molecular Biology series provides a collection of protocols for many of the common experimental approaches in plant developmental bi- ogy. All chapters are written in the same format as that used in the Methods in Molecular TM Biology series. Each chapter opens with a description of the basic theory behind the method being described.

Editors and Affiliations

  • (ETH), Department of Biology, Swiss Federal Institute of Technology, Zurich, Switzerland

    Lars Hennig, Claudia Köhler

Bibliographic Information

  • Book Title: Plant Developmental Biology

  • Book Subtitle: Methods and Protocols

  • Editors: Lars Hennig, Claudia Köhler

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-60761-765-5

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2010

  • Hardcover ISBN: 978-1-60761-764-8Published: 11 June 2010

  • Softcover ISBN: 978-1-4939-5734-7Published: 23 August 2016

  • eBook ISBN: 978-1-60761-765-5Published: 24 August 2010

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 448

  • Topics: Plant Sciences, Plant Genetics and Genomics, Developmental Biology

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