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Circulating Tumor Cells

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Mark Jesus M. Magbanua

    1. Division of Hematology Oncology, Helen Diller Family Comprehensive Cancer Center, University of California-San Francisco, San Francisco, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. John W. Park

    1. Division of Hematology Oncology, Helen Diller Family Comprehensive Cancer Center, University of California-San Francisco, San Francisco, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1634)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xxv
    Download chapter PDF

  2. Microfluidic Capture and Multiplex Immunofluorescence of Circulating Tumor Cells to Identify Cancer of Origin

    • Chian-Hui Lai, Ying-Chih Chang
    Pages 1-19

  3. Microfluidic Separation of Circulating Tumor Cells Based on Size and Deformability

    • Emily S. Park, Simon P. Duffy, Hongshen Ma
    Pages 21-32

  4. A Novel Microfluidic Device for Isolation of Circulating Tumor Cells from Pancreatic Cancer Blood Samples

    • Jose I. Varillas, Kangfu Chen, Jinling Zhang, Thomas J. George Jr., Z. Hugh Fan
    Pages 33-53

  5. Microfluidic-Based Enrichment and Retrieval of Circulating Tumor Cells for RT-PCR Analysis

    • Priya Gogoi, Saedeh Sepehri, Will Chow, Kalyan Handique, Yixin Wang
    Pages 55-64

  6. Microscale Laminar Vortices for High-Purity Extraction and Release of Circulating Tumor Cells

    • Soojung Claire Hur, James Che, Dino Di Carlo
    Pages 65-79

  7. Separable Bilayer Microfiltration Device for Label-Free Enrichment of Viable Circulating Tumor Cells

    • Sijie Hao, Merisa Nisic, Hongzhang He, Yu-Chong Tai, Si-Yang Zheng
    Pages 81-91

  8. Microfilter-Based Capture and Release of Viable Circulating Tumor Cells

    • Siddarth Rawal, Zheng Ao, Ram H. Datar, Ashutosh Agarwal
    Pages 93-105

  9. Fourier Ptychographic Microscopy for Rapid, High-Resolution Imaging of Circulating Tumor Cells Enriched by Microfiltration

    • Anthony Williams, Jaebum Chung, Changhuei Yang, Richard J. Cote
    Pages 107-117

  10. Automated Microfluidic Filtration and Immunocytochemistry Detection System for Capture and Enumeration of Circulating Tumor Cells and Other Rare Cell Populations in Blood

    • Michael Pugia, Mark Jesus M. Magbanua, John W. Park
    Pages 119-131

  11. Filter-Adapted Fluorescent In Situ Hybridization (FA-FISH) for Filtration-Enriched Circulating Tumor Cells

    • Marianne Oulhen, Emma Pailler, Vincent Faugeroux, Françoise Farace
    Pages 133-141

  12. Negative Enrichment and Isolation of Circulating Tumor Cells for Whole Genome Amplification

    • Nisha Kanwar, Susan J. Done
    Pages 143-152

  13. Capture and Genetic Analysis of Circulating Tumor Cells Using a Magnetic Separation Device (Magnetic Sifter)

    • Chin Chun Ooi, Seung-min Park, Dawson J. Wong, Sanjiv S. Gambhir, Shan X. Wang
    Pages 153-162

  14. RareCyte® CTC Analysis Step 1: AccuCyte® Sample Preparation for the Comprehensive Recovery of Nucleated Cells from Whole Blood

    • Arturo B. Ramirez, Lance U’Ren, Daniel E. Campton, David Stewart, Joshua J. Nordberg, Jackie L. Stilwell et al.
    Pages 163-172

  15. RareCyte® CTC Analysis Step 2: Detection of Circulating Tumor Cells by CyteFinder® Automated Scanning and Semiautomated Image Analysis

    • Jeffrey L. Werbin, Joshua J. Nordberg, Jay Tzucker, Paulina Varshavskaya, Jackie L. Stilwell, Eric P. Kaldjian
    Pages 173-180

  16. RareCyte® CTC Analysis Step 3: Using the CytePicker® Module for Individual Cell Retrieval and Subsequent Whole Genome Amplification of Circulating Tumor Cells for Genomic Analysis

    • Jackie L. Stilwell, Paulina Varshavskaya, Jeffrey L. Werbin, Joshua J. Nordberg, Arturo B. Ramirez, Steve Quarre et al.
    Pages 181-192

  17. Enumeration, Dielectrophoretic Capture, and Molecular Analysis of Circulating Tumor Cells

    • Stephanie S. Yee, Erica L. Carpenter
    Pages 193-202

  18. Enumeration of Circulating Tumor Cells and Disseminated Tumor Cells in Blood and Bone Marrow by Immunomagnetic Enrichment and Flow Cytometry (IE/FC)

    • Mark Jesus M. Magbanua, Tulasi I. Solanki, Andrea D. Ordonez, Feng Hsiao, John W. Park
    Pages 203-210

  19. Flow Cytometric Detection of Circulating Tumor Cells Using a Candidate Stem Cell Marker, p75 Neurotrophin Receptor (p75NTR)

    • Tomoyuki Okumura, Tetsuji Yamaguchi, Toru Watanabe, Takuya Nagata, Yutaka Shimada
    Pages 211-217

  20. Multispectral Imaging Analysis of Circulating Tumor Cells in Negatively Enriched Peripheral Blood Samples

    • Brandon Miller, Maryam Lustberg, Thomas A. Summers, Jeffrey J. Chalmers
    Pages 219-234
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Keywords

About this book

This volume explores various approaches for enrichment, detection, isolation, and molecular profiling of circulating tumor cells (CTCs). Each chapter provides comprehensive descriptions and guidelines on how to perform innovative experiments in CTC research. Included are protocols for capture of CTCs via filtration and density gradient centrifugation; microfluidic and immunomagnetic separation of CTCs; detection of CTCs by immunocytochemistry, fluorescence in situ hybridization, and flow cytometry; assays designed for genomic characterization and functional analyses of CTCs, and many more. Written in the highly successful Methods in Molecular Biology series format, the chapters in this book include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Cutting-edge and authoritative, Circula

ting Tumor Cells: Methods and Protocols is a valuable resource for laboratory researchers and clinicians who are interested in furthering their studies on CTCs.

Editors and Affiliations

  • Division of Hematology Oncology, Helen Diller Family Comprehensive Cancer Center, University of California-San Francisco, San Francisco, USA

    Mark Jesus M. Magbanua, John W. Park

Bibliographic Information

  • Book Title: Circulating Tumor Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Mark Jesus M. Magbanua, John W. Park

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7144-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-7143-5Published: 18 August 2017

  • Softcover ISBN: 978-1-4939-8397-1Published: 03 August 2018

  • eBook ISBN: 978-1-4939-7144-2Published: 17 August 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XXV, 306

  • Number of Illustrations: 21 b/w illustrations, 75 illustrations in colour

  • Topics: Cancer Research

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