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Cervical Cancer

Methods and Protocols

  • Book
  • © 2015

Overview

Editors:
  1. Daniel Keppler

    1. Touro University California, Vallejo, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Athena W. Lin

    1. Touro University California, Vallejo, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols for researching cervical cancer and high-risk HPV types
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1249)

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Table of contents (29 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Approaches to the Detection and Analysis of HPV Types

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Evolution and Classification of Oncogenic Human Papillomavirus Types and Variants Associated with Cervical Cancer

      • Zigui Chen, Luciana Bueno de Freitas, Robert D. Burk
      Pages 3-26

    3. A Real-Time PCR Approach Based on SPF10 Primers and the INNO-LiPA HPV Genotyping Extra Assay for the Detection and Typing of Human Papillomavirus

      • M. Isabel Micalessi, Gaëlle A. Boulet, Johannes Bogers
      Pages 27-35

  3. Molecular Pathogenesis of CxCA

    1. Front Matter

      Pages 37-37
      Download chapter PDF

    2. Replication of Human Papillomavirus in Culture

      • Eric J. Ryndock, Jennifer Biryukov, Craig Meyers
      Pages 39-52

    3. HPV Binding Assay to Laminin-332/Integrin α6β4 on Human Keratinocytes

      • Sarah A. Brendle, Neil D. Christensen
      Pages 53-66

    4. Methods to Assess the Nucleocytoplasmic Shuttling of the HPV E1 Helicase and Its Effects on Cellular Proliferation and Induction of a DNA Damage Response

      • Michaël Lehoux, Amélie Fradet-Turcotte, Jacques Archambault
      Pages 67-80

    5. Genetic Methods for Studying the Role of Viral Oncogenes in the HPV Life Cycle

      • Jason M. Bodily
      Pages 81-91

    6. Robust HPV-18 Production in Organotypic Cultures of Primary Human Keratinocytes

      • Hsu-Kun Wang, Thomas R. Broker, Louise T. Chow
      Pages 93-109

    7. A High-Throughput Cellular Assay to Quantify the p53-Degradation Activity of E6 from Different Human Papillomavirus Types

      • David Gagnon, Jacques Archambault
      Pages 111-120

    8. Retroviral Expression of Human Cystatin Genes in HeLa Cells

      • Crystal M. Diep, Gagandeep Kaur, Daniel Keppler, Athena W. Lin
      Pages 121-131

  4. Immune Responses to HPV Infections

    1. Front Matter

      Pages 133-133
      Download chapter PDF

    2. Molecular Analysis of Human Papillomavirus Virus-Like Particle Activated Langerhans Cells In Vitro

      • Andrew W. Woodham, Adam B. Raff, Diane M. Da Silva, W. Martin Kast
      Pages 135-149

  5. Identification of Novel Potential Drug Targets

    1. Front Matter

      Pages 151-151
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    2. Selective Silencing of Gene Target Expression By siRNA Expression Plasmids in Human Cervical Cancer Cells

      • Oscar Peralta-Zaragoza, Faustino De-la-O-Gómez, Jessica Deas, Gloria Fernández-Tilapa, Geny del Socorro Fierros-Zárate, Claudia Gómez-Cerón et al.
      Pages 153-171

    3. Silencing of E6/E7 Expression in Cervical Cancer Stem-Like Cells

      • Wenyi Gu, Nigel McMillan, Chengzhong Yu
      Pages 173-182

    4. Two-Step Procedure for Evaluating Experimentally Induced DNA Damage: Texas Red and Comet Assays

      • Gina J. Ferris, Lauren B. Shunkwiler, Charles A. Kunos
      Pages 183-191

    5. Measurement of Deubiquitinating Enzyme Activity Via a Suicidal HA-Ub-VS Probe

      • Colleen Rivard, Martina Bazzaro
      Pages 193-200

  6. Approaches to Detecting, Typing, and Staging of CxCA

    1. Front Matter

      Pages 201-201
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Keywords

About this book

Representing the most relevant procedures and technologies aiding the advance of the field of HPV-mediated carcinogenesis of the cervix and other anatomical regions of squamocolumnar transition, such as the anorectum, penis, and oropharynx, Cervical Cancer: Methods and Protocols compiles a detailed collection of practical chapters. The first half of the book covers HPV types, pathogenesis of cervical cancer (CxCA), prevention, and novel potential drug targets, while the second half explores pathology, genomics, modeling of CxCA, and experimental therapeutic strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and vital, Cervical Cancer: Methods and Protocols serves as a valuable resource to both bench scientists and clinicians who step into the realm of high-risk HPVs and CxCA for the first time or those who wish to learn novel approaches or expand their toolbox for the study of CxCA.

Editors and Affiliations

  • Touro University California, Vallejo, USA

    Daniel Keppler, Athena W. Lin

Bibliographic Information

  • Book Title: Cervical Cancer

  • Book Subtitle: Methods and Protocols

  • Editors: Daniel Keppler, Athena W. Lin

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2013-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2012-9Published: 28 October 2014

  • Softcover ISBN: 978-1-4939-5285-4Published: 22 September 2016

  • eBook ISBN: 978-1-4939-2013-6Published: 27 October 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 413

  • Number of Illustrations: 28 b/w illustrations, 42 illustrations in colour

  • Topics: Cancer Research, Pharmacy

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