Skip to main content
SpringerLink
Log in
Book cover

Laser Capture Microdissection

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Graeme I. Murray

    1. School of Medicine, Medical Sciences and Nutrition, University of Aberdeen, Aberdeen, United Kingdom

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols involving laser capture microdissection
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1723)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 129.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (23 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Laser Capture Microdissection: Insights into Methods and Applications

    • Meera Mahalingam
    Pages 1-17

  3. Laser Microdissection-Based Microproteomics of Formalin-Fixed and Paraffin-Embedded (FFPE) Tissues

    • Rémi Longuespée, Dominique Baiwir, Gabriel Mazzucchelli, Nicolas Smargiasso, Edwin De Pauw
    Pages 19-31

  4. Laser Microdissection Workflow for Isolating Nucleic Acids from Fixed and Frozen Tissue Samples

    • Yelena G. Golubeva, Andrew C. Warner
    Pages 33-93

  5. Protocol for the Analysis of Laser Capture Microdissected Fresh-Frozen Tissue Homogenates by Silver-Stained 1D SDS-PAGE

    • DaRue A. Prieto, Gordon Whitely, Donald J. Johann Jr., Josip Blonder
    Pages 95-110

  6. Next-Generation Sequencing Analysis of Laser-Microdissected Formalin-Fixed and Paraffin-Embedded (FFPE) Tissue Specimens

    • Lavinia Mägel, Stephan Bartels, Ulrich Lehmann
    Pages 111-118

  7. Adaptation of Laser Microdissection Technique to Nanostring RNA Analysis in the Study of a Spontaneous Metastatic Mammary Carcinoma Mouse Model

    • Nadia P. Castro, Yelena G. Golubeva
    Pages 119-137

  8. Laser Capture Microdissection as a Tool to Study the Mucosal Immune Response in Celiac Disease

    • Giuseppe Iacomino, Vera Rotondi Aufiero, Pasquale Marena, Antonella Venezia, Riccardo Troncone, Salvatore Auricchio et al.
    Pages 139-154

  9. Laser Capture Microdissection and Isolation of High-Quality RNA from Frozen Endometrial Tissue

    • Michele Cummings, Georgia Mappa, Nicolas M. Orsi
    Pages 155-166

  10. Laser Microdissection for Human Papillomavirus (HPV) Genotyping Attribution and Methylation Pattern Analyses of Squamous Intraepithelial Lesions

    • Monica Molano, Suzanne M. Garland, Alyssa M. Cornall
    Pages 167-189

  11. Laser Capture Microdissection and Transcriptional Analysis of Sub-Populations of the Osteoblast Lineage from Undecalcified Bone

    • Efrain Pacheco, Rong Hu, Scott Taylor
    Pages 191-202

  12. Cell Type-Specific Laser Capture Microdissection for Gene Expression Profiling in the Human Brain

    • Sarah A. Mauney, Tsung-Ung W. Woo, Kai C. Sonntag
    Pages 203-221

  13. The Isolation of Pure Populations of Neurons by Laser Capture Microdissection: Methods and Application in Neuroscience

    • Renée Morris, Prachi Mehta
    Pages 223-233

  14. Laser Capture Microdissection in Traumatic Brain Injury Research: Obtaining Hippocampal Subregions and Pools of Injured Neurons for Genomic Analyses

    • Deborah R. Boone, Harris A. Weisz, Stacy L. Sell, Helen L. Hellmich
    Pages 235-245

  15. Isolation of Distinct Types of Neurons from Fresh Brain Tissue Using Laser Microdissection in Combination with High-Performance Liquid Chromatography—Mass Spectrometry

    • Luisa Aring, Simone Steinbach, Katrin Marcus, Caroline May
    Pages 247-260

  16. Immuno-Guided Laser-Capture Microdissection of Glial Cells for mRNA Analysis

    • Arnaud B. Nicot, Justine Rambeau, Flora Guillot, Alexandra Garcia, David A. Laplaud
    Pages 261-271

  17. Immuno-Laser-Capture Microdissection for the Isolation of Enriched Glial Populations from Frozen Post-Mortem Human Brain

    • Julie E. Simpson, Stephen B. Wharton, Paul R. Heath
    Pages 273-284

  18. Laser-Capture Microdissection for the Analysis of Rat and Human Spinal Cord Ependyma by qPCR

    • Daniel Garcia-Ovejero, Beatriz Paniagua-Torija, Angel Arevalo-Martin, Beatriz Navarro-Galve, Eduardo Molina-Holgado
    Pages 285-318

  19. Isolation of Amyloid Plaques and Neurofibrillary Tangles from Archived Alzheimer’s Disease Tissue Using Laser-Capture Microdissection for Downstream Proteomics

    • Eleanor Drummond, Shruti Nayak, Geoffrey Pires, Beatrix Ueberheide, Thomas Wisniewski
    Pages 319-334

  20. Cell-Specific RNA Quantification in Human SN DA Neurons from Heterogeneous Post-mortem Midbrain Samples by UV-Laser Microdissection and RT-qPCR

    • Johanna Duda, Michael Fauler, Jan Gründemann, Birgit Liss
    Pages 335-360
Back to top

Keywords

About this book

This fully updated edition provides practical advice on how to carry out tissue-based laser microdissection successfully by using the different laser microdissection systems that are available and by applying a wide range of molecular technologies. The contents of the volume explore these techniques that have revolutionized carrying out molecular analysis on specific types of normal and diseased cells and fully utilizing the power of current molecular technologies including PCR, microarrays, next generation sequencing, and proteomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and comprehensive, Laser Capture Microdissection: Methods and Protocols, Third Edition seeks to aid researchers generally and pathologists in particular in moving their studies forward with these vital techniques.

Editors and Affiliations

  • School of Medicine, Medical Sciences and Nutrition, University of Aberdeen, Aberdeen, United Kingdom

    Graeme I. Murray

Bibliographic Information

  • Book Title: Laser Capture Microdissection

  • Book Subtitle: Methods and Protocols

  • Editors: Graeme I. Murray

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7558-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2018

  • Hardcover ISBN: 978-1-4939-7557-0Published: 17 January 2018

  • Softcover ISBN: 978-1-4939-8520-3Published: 04 June 2019

  • eBook ISBN: 978-1-4939-7558-7Published: 17 January 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XV, 412

  • Number of Illustrations: 16 b/w illustrations, 60 illustrations in colour

  • Topics: Biotechnology

Publish with us

Policies and ethics

Back to top

Search

Navigation