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Flow Cytometry Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Teresa S. Hawley

    1. Flow Cytometry Section, Research Technologies Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Robert G. Hawley

    1. Department of Anatomy and Regenerative Biology, School of Medicine and Health Sciences, George Washington University, Washington, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1678)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Flow Cytometry: The Glass Is Half Full

    • Howard M. Shapiro
    Pages 1-10

  3. High-Dimensional Modeling for Cytometry: Building Rock Solid Models Using GemStone™ and Verity Cen-se’™ High-Definition t-SNE Mapping

    • C. Bruce Bagwell
    Pages 11-36

  4. Mass Cytometry Assays for Antigen-Specific T Cells Using CyTOF

    • Dongxia Lin, Holden T. Maecker
    Pages 37-47

  5. RNA Flow Cytometry Using the Branched DNA Technique

    • Kah Teong Soh, Paul K. Wallace
    Pages 49-77

  6. Analysis of Individual Extracellular Vesicles by Flow Cytometry

    • John P. Nolan, Erika Duggan
    Pages 79-92

  7. Quantitative Fluorescence Measurements with Multicolor Flow Cytometry

    • Lili Wang, Adolfas K. Gaigalas, James Wood
    Pages 93-110

  8. High Throughput Flow Cytometry for Cell Surface Profiling

    • Joshua Paterson, Laurie E. Ailles
    Pages 111-138

  9. Multiparameter Conventional Flow Cytometry

    • Katherine M. McKinnon
    Pages 139-150

  10. Multiparameter Intracellular Cytokine Staining

    • Patricia Lovelace, Holden T. Maecker
    Pages 151-166

  11. Multiparametric Analysis of Apoptosis by Flow Cytometry

    • William G. Telford
    Pages 167-202

  12. Multiparameter Cell Cycle Analysis

    • James W. Jacobberger, R. Michael Sramkoski, Tammy Stefan, Philip G. Woost
    Pages 203-247

  13. Monitoring Cell Proliferation by Dye Dilution: Considerations for Probe Selection

    • Joseph D. Tario Jr., Alexis N. Conway, Katharine A. Muirhead, Paul K. Wallace
    Pages 249-299

  14. Immunophenotypic Identification of Early Myeloerythroid Development

    • Cornelis J. H. Pronk, David Bryder
    Pages 301-319

  15. Flow Cytometry Assays in Primary Immunodeficiency Diseases

    • Maurice. R. G. O’Gorman
    Pages 321-345

  16. Real-Time Deformability Cytometry: Label-Free Functional Characterization of Cells

    • Maik Herbig, Martin Kräter, Katarzyna Plak, Paul Müller, Jochen Guck, Oliver Otto
    Pages 347-369

  17. Nuclear Cytometry: Analysis of the Patterns of DNA Synthesis and Transcription Using Flow Cytometry, Confocal Microscopy, and RNA Sequencing

    • David W. Galbraith, Elwira Sliwinska, Partha Samadder
    Pages 371-392

  18. Flow Cytometric FRET Analysis of Protein Interactions

    • László Ujlaky-Nagy, Péter Nagy, János SzöllÅ‘si, György Vereb
    Pages 393-419

  19. Overview of Fluorescence Lifetime Measurements in Flow Cytometry

    • Jessica P. Houston, Zhihua Yang, Jesse Sambrano, Wenyan Li, Kapil Nichani, Giacomo Vacca
    Pages 421-446

  20. Overview of Lasers for Flow Cytometry

    • William G. Telford
    Pages 447-479
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Keywords

About this book

This fourth edition volume expands on the previous editions by presenting readers with new and emerging methodologies in cytometry. The chapters in this book cover various topics such as quantifying surface and intracellular protein biomarkers, intracellular cytokine staining, apoptosis analysis, cell cycle analysis, tracking cell proliferation, and monitoring protein-protein interactions using FRET. Some developing methodologies that explored are mass cytometry, vesicle flow cytometry, time-resolved flow cytometry, and real-time label-free deformability cytometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Flow Cytometry Protocols, Fourth Edition  researchers and scientists who are interested in continuing or expanding their knowledge of flow cytometry.

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Editors and Affiliations

  • Flow Cytometry Section, Research Technologies Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, USA

    Teresa S. Hawley

  • Department of Anatomy and Regenerative Biology, School of Medicine and Health Sciences, George Washington University, Washington, USA

    Robert G. Hawley

About the editors

 

Bibliographic Information

  • Book Title: Flow Cytometry Protocols

  • Editors: Teresa S. Hawley, Robert G. Hawley

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7346-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2018

  • Hardcover ISBN: 978-1-4939-7344-6Published: 26 October 2017

  • Softcover ISBN: 978-1-4939-8459-6Published: 23 August 2018

  • eBook ISBN: 978-1-4939-7346-0Published: 24 October 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 4

  • Number of Pages: XIV, 492

  • Number of Illustrations: 27 b/w illustrations, 80 illustrations in colour

  • Topics: Biotechnology

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