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Microbial Steroids

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. José-Luis Barredo

    1. Department of Biotechnology, Crystal Pharma, A Division of Albany Molecular Research Inc. (AMRI), Parque Tecnológico de León, León, Spain

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  2. Ignacio Herráiz

    1. Research and Development Department, Gadea Pharmaceutical Group, A Division of Albany Molecular Research Inc. (AMRI), Parque Tecnológico de Boecillo, Boecillo, Spain

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1645)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Steroid Bioconversions

    • Marina V. Donova
    Pages 1-13

  3. Chemical Pathways of Corticosteroids, Industrial Synthesis from Sapogenins

    • Ignacio Herráiz
    Pages 15-27

  4. Genome Sequencing of Steroid-Producing Bacteria with Illumina Technology

    • Victoria Y. Shtratnikova, Mikhail I. Schelkunov, Marina V. Donova
    Pages 29-44

  5. Genome Sequencing of Steroid Producing Bacteria Using Ion Torrent Technology and a Reference Genome

    • Alberto Sola-Landa, Antonio Rodríguez-García, Carlos Barreiro, Rosario Pérez-Redondo
    Pages 45-58

  6. RNA-Seq-Based Comparative Transcriptomics: RNA Preparation and Bioinformatics

    • Antonio Rodríguez-García, Alberto Sola-Landa, Carlos Barreiro
    Pages 59-72

  7. Intra- and Extra-cellular Proteome Analyses of Steroid-Producer Mycobacteria

    • Carlos Barreiro, Alejandro Morales, Inés Vázquez-Iglesias, Alberto Sola-Landa
    Pages 73-92

  8. Genetic Techniques for Manipulation of the Phytosterol Biotransformation Strain Mycobacterium neoaurum NRRL B-3805

    • Jessica K. Loraine, Margaret C. M. Smith
    Pages 93-108

  9. Identification and Characterization of the Genes and Enzymes Belonging to the Bile Acid Catabolic Pathway in Pseudomonas

    • José M. Luengo, Elías R. Olivera
    Pages 109-142

  10. Selection of Biodegrading Phytosterol Strains

    • María-Angélica Mondaca, Maricel Vidal, Soledad Chamorro, Gladys Vidal
    Pages 143-150

  11. Stigmasterol Removal by an Aerobic Treatment System

    • Soledad Chamorro, Claudia Xavier, Víctor Hernández, José Becerra, Gladys Vidal
    Pages 151-158

  12. Production and Biotransformation of Phytosterol Microdispersions to Produce 4-Androstene-3,17-Dione

    • Rodrigo A. Mancilla, Roberto Pavez-Díaz, Alejandro Amoroso
    Pages 159-165

  13. β-Sitosterol Bioconversion to Androstenedione in Microtiter Plates

    • Marco P. C. Marques, Pedro Fernandes
    Pages 167-176

  14. Bioconversion of Phytosterols into Androstenedione by Mycobacterium

    • Kjell D. Josefsen, Anna Nordborg, Håvard Sletta
    Pages 177-197

  15. Scale-Up of Phytosterols Bioconversion into Androstenedione

    • Sonia Martínez-Cámara, Esther Bahíllo, José-Luis Barredo, Marta Rodríguez-Sáiz
    Pages 199-210

  16. Bioconversion of Phytosterols into Androstadienedione by Mycobacterium smegmatis CECT 8331

    • Julia García-Fernández, Igor Martínez, Lorena Fernández-Cabezón, Carmen Felpeto-Santero, José-Luis García, Beatriz Galán
    Pages 211-225

  17. Process for Biotransformation of Androsta-4-ene-3, 17-Dione (4-AD) to Androsta-1,4-Diene-3,17-Dione (ADD)

    • Surya Prakash, Abhay Bajaj
    Pages 227-238

  18. Laboratory-Scale Hydroxylation of Steroids by P450BM3 Variants

    • Sabrina Hoebenreich, Martin Spinck, Nathalie Nett
    Pages 239-257

  19. Obtaining of 11α-Hydroxyandrost-4-ene-3,17-dione from Natural Sterols

    • Dmitry Dovbnya, Sergey Khomutov, Vyacheslav Kollerov, Marina V. Donova
    Pages 259-269

  20. Steroid 11-Alpha-Hydroxylation by the Fungi Aspergillus nidulans and Aspergillus ochraceus

    • Lidia Ortega-de los Ríos, José M. Luengo, José M. Fernández-Cañón
    Pages 271-287
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Keywords

About this book

This volume provides practical experimental laboratory protocols for a wide range of steroid bioconversions. The chapters in this book cover topics such as bioconversions and chemical synthesis pathways; strain characterization; bioconversion from sterols to androstenedione and androstadienedione; steroid hydroxylations; biocatalysis; and downstream processes to purify steroid intermediates. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and thorough, Microbial Steroids: Methods and Protocols is a valuable resource for laboratory and industrial professionals. It is also useful for graduate students studying biotechnology, microbiology, genetics, and molecular biology.

Editors and Affiliations

  • Department of Biotechnology, Crystal Pharma, A Division of Albany Molecular Research Inc. (AMRI), Parque Tecnológico de León, León, Spain

    José-Luis Barredo

  • Research and Development Department, Gadea Pharmaceutical Group, A Division of Albany Molecular Research Inc. (AMRI), Parque Tecnológico de Boecillo, Boecillo, Spain

    Ignacio Herráiz

Bibliographic Information

  • Book Title: Microbial Steroids

  • Book Subtitle: Methods and Protocols

  • Editors: José-Luis Barredo, Ignacio Herráiz

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7183-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-7182-4Published: 15 July 2017

  • Softcover ISBN: 978-1-4939-8410-7Published: 08 August 2018

  • eBook ISBN: 978-1-4939-7183-1Published: 14 July 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 373

  • Number of Illustrations: 20 b/w illustrations, 27 illustrations in colour

  • Topics: Biochemistry, general

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