Kinase Signaling Networks

1. Front Matter

   Pages i-xvii

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2. Synthetic Biology, Chemical Biology, and Screening Approaches

   1. Front Matter

      Pages 1-1

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   2. Optogenetic Control of Ras/Erk Signaling Using the Phy–PIF System

      • Alexander G. Goglia, Maxwell Z. Wilson, Daniel B. DiGiorno, Jared E. Toettcher

      Pages 3-20

   3. Dissecting Kinase Effector Signaling Using the RapRTAP Methodology

      • Anne-Marie Ray, Jennifer E. Klomp, Kerrie B. Collins, Andrei V. Karginov

      Pages 21-33

   4. Single-Cell Imaging of ERK Signaling Using Fluorescent Biosensors

      • Michael Pargett, Taryn E. Gillies, Carolyn K. Teragawa, Breanne Sparta, John G. Albeck

      Pages 35-59

   5. Quantification of Cell Signaling Networks Using Kinase Activity Chemosensors

      • Jon R. Beck, Edward N. Harris, Cliff I. Stains

      Pages 61-70

   6. Expression of Recombinant Phosphoproteins for Signal Transduction Studies

      • Karl W. Barber, Jesse Rinehart

      Pages 71-78

   7. Allosteric Modulation of Src Family Kinases with ATP-Competitive Inhibitors

      • Ames C. Register, Sujata Chakraborty, Dustin J. Maly

      Pages 79-89

   8. Characterization of Ligand Binding to Pseudokinases Using a Thermal Shift Assay

      • Isabelle S. Lucet, James M. Murphy

      Pages 91-104

   9. Proteomic Profiling of Protein Kinase Inhibitor Targets by Mass Spectrometry

      • Martin Golkowski, Dustin J. Maly, Shao-En Ong

      Pages 105-117

   10. Utilizing the Luminex Magnetic Bead-Based Suspension Array for Rapid Multiplexed Phosphoprotein Quantification

       • Adam Stewart, Udai Banerji

       Pages 119-131

   11. High-Content Imaging and RNAi Screens for Investigating Kinase Network Plasticity

       • Simon R. Stockwell, Sibylle Mittnacht

       Pages 133-161

   12. Analysis of Drug Resistance Using Kinome-Wide Functional Screens

       • Katherine R. Singleton, Keith T. Earley, Lynn E. Heasley

       Pages 163-177

   13. Identification and Validation of Driver Kinases from Next-Generation Sequencing Data

       • Andri Leonidou, Barrie Peck, Rachael Natrajan

       Pages 179-195

3. Mass Spectrometry and Metabolic Analysis of Kinase Signaling

   1. Front Matter

      Pages 197-197

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   2. Label-Free Phosphoproteomic Approach for Kinase Signaling Analysis

      • Edmund Wilkes, Pedro R. Cutillas

      Pages 199-217

   3. Cell-Specific Labeling for Analyzing Bidirectional Signaling by Mass Spectrometry

      • Christopher J. Tape, Claus Jørgensen

      Pages 219-234

   4. Characterization of the Phospho-Adhesome by Mass Spectrometry-Based Proteomics

      • Joseph Robertson, Jonathan D. Humphries, Nikki R. Paul, Stacey Warwood, David Knight, Adam Byron et al.

      Pages 235-251

   5. Analysis of Phosphotyrosine Signaling Networks in Lung Cancer Cell Lines

      • Malgorzata Broncel, Paul H. Huang

      Pages 253-262

   6. Targeted Analysis of Phosphotyrosine Signaling by Multiple Reaction Monitoring Mass Spectrometry

      • Leo S. Payne, Paul H. Huang

      Pages 263-281

This detailed volume describes cutting-edge techniques in three distinct and complementary areas of contemporary kinase biology research. Beginning with a section on synthetic biology, chemical biology, and screening approaches to kinase signaling networks, the book continues with sections on mass spectrometry and metabolic analysis of kinase signaling as well as computational analysis of kinase signaling networks. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Kinase Signaling Networks aims to provide scientists with the tools necessary to overcome the technological bottlenecks that limit our ability to gain a deeper mechanistic understanding of kinase biology.

• Protein science
• Synthetic biology
• Screening approaches
• Mass spectrometry
• Computational analysis
• Chemical biology
• Drug development

• Division of Medical Oncology, University of Colorado, Aurora, USA

  Aik-Choon Tan

• Protein Networks Laboratory, Institute of Cancer Research, London, United Kingdom

  Paul H. Huang

Policies and ethics