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Microbial Toxins

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Otto Holst

    1. Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Borstel, Germany

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1600)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Detection of Cholera Toxin by an Immunochromatographic Test Strip

    • Eiki Yamasaki, Ryuta Sakamoto, Takashi Matsumoto, Biswajit Maiti, Kayo Okumura, Fumiki Morimatsu et al.
    Pages 1-7

  3. Electrochemical Aptamer Scaffold Biosensors for Detection of Botulism and Ricin Proteins

    • Jessica Daniel, Lisa Fetter, Susan Jett, Teisha J. Rowland, Andrew J. Bonham
    Pages 9-23

  4. A Cell-Based Fluorescent Assay to Detect the Activity of AB Toxins that Inhibit Protein Synthesis

    • Patrick Cherubin, Beatriz Quiñones, Salem Elkahoui, Wallace Yokoyama, Ken Teter
    Pages 25-36

  5. Molecular Methods for Identification of Clostridium tetani by Targeting Neurotoxin

    • Basavraj Nagoba, Mahesh Dharne, Kushal N. Gohil
    Pages 37-47

  6. Label-Free Immuno-Sensors for the Fast Detection of Listeria in Food

    • Alexandra Morlay, Agnès Roux, Vincent Templier, Félix Piat, Yoann Roupioz
    Pages 49-59

  7. Aptamer-Based Trapping: Enrichment of Bacillus cereus Spores for Real-Time PCR Detection

    • Christin Fischer, Markus Fischer
    Pages 61-68

  8. Detection of Yersinia pestis in Complex Matrices by Intact Cell Immunocapture and Targeted Mass Spectrometry

    • Jérôme Chenau, François Fenaille, Stéphanie Simon, Sofia Filali, Hervé Volland, Christophe Junot et al.
    Pages 69-83

  9. A Method to Prepare Magnetic Nanosilicate Platelets for Effective Removal of Microcystis aeruginosa and Microcystin-LR

    • Shu-Chi Chang, Bo-Li Lu, Jiang-Jen Lin, Yen-Hsien Li, Maw-Rong Lee
    Pages 85-94

  10. An Immunochromatographic Test Strip to Detect Ochratoxin A and Zearalenone Simultaneously

    • Xiaofei Hu, Gaiping Zhang
    Pages 95-105

  11. Endotoxin Removal from Escherichia coli Bacterial Lysate Using a Biphasic Liquid System

    • Janusz Boratyński, Bożena Szermer-Olearnik
    Pages 107-112

  12. Fourier Transform Infrared Spectroscopy as a Tool in Analysis of Proteus mirabilis Endotoxins

    • Paulina Żarnowiec, Grzegorz Czerwonka, Wiesław Kaca
    Pages 113-124

  13. Laser Interferometry Method as a Novel Tool in Endotoxins Research

    • Michał Arabski, Sławomir Wąsik
    Pages 125-132

  14. Endotoxin Entrapment on Glass via C-18 Self-Assembled Monolayers and Rapid Detection Using Drug-Nanoparticle Bioconjugate Probes

    • Prasanta Kalita, Anshuman Dasgupta, Shalini Gupta
    Pages 133-142

  15. A Bioassay for the Determination of Lipopolysaccharides and Lipoproteins

    • Marcus Peters, Petra Bonowitz, Albrecht Bufe
    Pages 143-150

  16. Capillary Electrophoresis Chips for Fingerprinting Endotoxin Chemotypes and Subclasses

    • Béla Kocsis, Lilla Makszin, Anikó Kilár, Zoltán Péterfi, Ferenc Kilár
    Pages 151-165

  17. Micromethods for Isolation and Structural Characterization of Lipid A, and Polysaccharide Regions of Bacterial Lipopolysaccharides

    • Alexey Novikov, Aude Breton, Martine Caroff
    Pages 167-186

  18. Mass Spectrometry for Profiling LOS and Lipid A Structures from Whole-Cell Lysates: Directly from a Few Bacterial Colonies or from Liquid Broth Cultures

    • Béla Kocsis, Anikó Kilár, Szandra Péter, Ágnes Dörnyei, Viktor Sándor, Ferenc Kilár
    Pages 187-198

  19. Back Matter

    Pages 199-201
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Keywords

About this book

This second edition volume expands on the previous editions with detailed protocols on (cyano) bacterial and mold fungus toxins, and a specific focus on Gram-negative endotoxins (lipopolysaccharides). The chapters in Microbial Toxins: Methods and Protocols, Second Edition present techniques that cover topics such as detection of cholera toxin by an immunochromatographic test strip; label-free immune-sensors for the fast detection of Listeria in food; endotoxin removal from Escherichia coli bacterial lysate using biphasic liquid system; and micromethods for isolation and structural characterization of lipid A, and polysaccharide regions of bacterial lipopolysaccharides. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Microbial Toxins: Methods and Protocols, Second Edition is a valuable and useful resource for scientists who are interested in this field, and is a great tool for researchers who are looking to learn about new, particular techniques to further enhance their work.

Editors and Affiliations

  • Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Borstel, Germany

    Otto Holst

Bibliographic Information

  • Book Title: Microbial Toxins

  • Book Subtitle: Methods and Protocols

  • Editors: Otto Holst

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6958-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6956-2Published: 06 May 2017

  • Softcover ISBN: 978-1-4939-8351-3Published: 20 July 2018

  • eBook ISBN: 978-1-4939-6958-6Published: 05 May 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 201

  • Number of Illustrations: 43 b/w illustrations, 28 illustrations in colour

  • Topics: Microbiology

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