Methods in Molecular Biology

Protocols in In Vitro Hepatocyte Research

Editors: Vinken, Mathieu, Rogiers, Vera (Eds.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook 91,62 €
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  • ISBN 978-1-4939-2074-7
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Hardcover 109,99 €
price for India (gross)
  • ISBN 978-1-4939-2073-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover 109,99 €
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  • ISBN 978-1-4939-4932-8
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  • Usually dispatched within 3 to 5 business days.
About this book

This volume presents 30 state-of-the-art protocols and reviews to set up and apply primary hepatocyte cultures for research and screening purposes. The first part of the book focuses on the use of these particular liver-based in vitro models to study the different aspects of the hepatocyte life cycle, including cell growth, differentiation and cell death. The second part of the book is targeted towards the demonstration of the applicability of primary hepatocyte cultures, or liver-based in vitro models derived thereof, for functionality and toxicity testing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Protocols In In-Vitro Hepatocyte Research is intended for basic and applied researchers in the area of pharmacology and toxicology, both in academic and industrial settings.  

Table of contents (20 chapters)

  • Isolation and Culture of Mouse Hepatocytes: Gender-Specific Gene Expression Responses to Chemical Treatments

    Lyndsey Houseman, Mina Edwards, Ian R. Phillips, Elizabeth A. Shephard

    Pages 3-12

    Preview Buy Chapter 39,95 €
  • Cryopreservation of Hepatocytes

    Nicola J. Hewitt, Albert P. Li

    Pages 13-26

    Preview Buy Chapter 39,95 €
  • Culture Conditions Promoting Hepatocyte Proliferation and Cell Cycle Synchronization

    Anne Corlu, Pascal Loyer

    Pages 27-51

    Preview Buy Chapter 39,95 €
  • Culture and Functional Characterization of Human Hepatoma HepG2 Cells

    María Teresa Donato, Laia Tolosa, María José Gómez-Lechón

    Pages 77-93

    Preview Buy Chapter 39,95 €
  • Serum-Free Directed Differentiation of Human Embryonic Stem Cells to Hepatocytes

    Kate Cameron, Baltasar Lucendo-Villarin, Dagmara Szkolnicka, David C. Hay

    Pages 105-111

    Preview Buy Chapter 39,95 €

Buy this book

eBook 91,62 €
price for India (gross)
  • ISBN 978-1-4939-2074-7
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover 109,99 €
price for India (gross)
  • ISBN 978-1-4939-2073-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover 109,99 €
price for India (gross)
  • ISBN 978-1-4939-4932-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Protocols in In Vitro Hepatocyte Research
Editors
  • Mathieu Vinken
  • Vera Rogiers
Series Title
Methods in Molecular Biology
Series Volume
1250
Copyright
2015
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-2074-7
DOI
10.1007/978-1-4939-2074-7
Hardcover ISBN
978-1-4939-2073-0
Softcover ISBN
978-1-4939-4932-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XVI, 406
Number of Illustrations and Tables
46 b/w illustrations, 26 illustrations in colour
Topics