Overview
- Provides classic, state of art, and cutting edge methods in a simple, easily reproducible format
- Contains a comprehensive set of experimental models including cell-free systems, different specialized cells, and model organisms
- Presents detailed lists of tested reagents that really work
- Notes on troubleshooting from leading laboratories in the field
Part of the book series: Methods in Molecular Biology (MIMB, volume 440)
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Table of contents (28 protocols)
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An Overview of Methods for Analyzing Vesicular Trafficking
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Cell-Free and Biochemical Assays of Exocytosis and Endocytosis
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Analysis of Endocytosis and Exocytosis in Different Cell Systems
Keywords
About this book
Due to their vital involvement in a wide variety of housekeeping and specialized cellular functions, exocytosis and endocytosis remain among the most popular subjects in biology and biomedical sciences. Tremendous progress in understanding these complex intracellular processes has been achieved by employing a wide array of research tools ranging from classical biochemical methods to modern imaging techniques. In Exocytosis and Endocytosis, skilled experts provide the most up-to-date,step-by-step laboratory protocols for examining molecular machinery and biological functions of exocytosis and endocytosis in vitro and in vivo. Following the highly successful Methods in Molecular Biologyâ„¢ series format, the chapters present an introduction outlining the principle behind each technique, a list of the necessary materials, an easy to follow, readily reproducible protocol, and a Notes section offering tips on troubleshooting and avoiding known pitfalls.
Insightful to both newcomers and seasoned professionals, Exocytosis and Endocytosis offers a unique and highly practical guide to versatile laboratory tools developed to study various aspects of intracellular vesicle trafficking in simple model systems and living organisms.
Editors and Affiliations
Bibliographic Information
Book Title: Exocytosis and Endocytosis
Editors: Andrei I. Ivanov
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-59745-178-9
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2008
Hardcover ISBN: 978-1-58829-865-2Published: 17 December 2007
Softcover ISBN: 978-1-61737-813-3Published: 10 December 2010
eBook ISBN: 978-1-59745-178-9Published: 15 February 2008
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XV, 412
Topics: Cell Biology, Human Physiology, Developmental Biology, Immunology, Neurobiology