Springer Lab Manuals

Antibody Engineering

Editors: Kontermann, Roland E., Dübel, Stefan (Eds.)

  • Comprehensive collection of proven lab protocols in the expanding field of antibody engineering
  • Beginners are introduced to the basic methods with detailed instructions
  • Professional antibody engineers find the latest approaches and emerging techniques
  • Each protocol is provided by an expert in this technology
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  • ISBN 978-3-662-04605-0
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About this book

Interest in recombinant antibody technologies has rapidly increased because of the wide range of possible applications in therapy and diagnosis, especially in cancer treatment. The possibility of generating human antibodies that are not accessible by conventional polyclonal or monoclonal approaches has forced the development of antibody engineering technologies even more.
This manual presents a comprehensive collection of detailed, step-by-step protocols provided by experts in the field. All basic methods needed in antibody engineering - not only methods to generate recombinant antibodies, but also protocols for analysis and their use - and recently developed and emerging technologies are covered. In particular, protocols on the following topics are provided:
Hybridoma immortalisation Generation and screening of antibody gene libraries from human donors, mice and rabbits Antibody selection on immunotubes, cells, tissues; proximity and step-back selections Creation of human monoclonal antibodies to toxic or highly pathogenic agents without immunisation Improvment of antibody binding Antibody humanisation Genetic fusions for the production of multifunctional antibody derivatives Radiolabelled recombinant antibodies Bispecific antibodies Antibody - enzyme fusions Intracellular antibodies Determination of affinity and specificity Computer analysis of antibody sequence and structure Epitope analysis by various phage display systems and peptide spot membranes Eukaryotic (plant, baculovirus, yeast, mammalian cells) and prokaryotic production systems for recombinant antibodies Purification systems Xenograft mice Emerging technologies

Table of contents (50 chapters)

  • Recombinant Antibodies

    Dübel, Stefan (et al.)

    Pages 3-16

  • Construction of scFv Fragments from Hybridoma or Spleen Cells by PCR Assembly

    Burmester, Jörg (et al.)

    Pages 19-40

  • Construction of scFv from Hybridoma by Two-Step Cloning

    Breitling, Frank (et al.)

    Pages 41-55

  • Cloning Hybridoma cDNA by RACE

    Bradbury, Andrew

    Pages 56-61

  • Phage — Display Libraries of Murine Antibody Fab Fragments

    Engberg, Jan (et al.)

    Pages 65-92

Buy this book

eBook n/a
  • ISBN 978-3-662-04605-0
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
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Bibliographic Information

Bibliographic Information
Book Title
Antibody Engineering
Editors
  • Roland E. Kontermann
  • Stefan Dübel
Series Title
Springer Lab Manuals
Copyright
2001
Publisher
Springer-Verlag Berlin Heidelberg
Copyright Holder
Springer-Verlag Berlin Heidelberg
eBook ISBN
978-3-662-04605-0
DOI
10.1007/978-3-662-04605-0
Edition Number
1
Number of Pages
XII, 790
Number of Illustrations and Tables
116 b/w illustrations, 9 illustrations in colour
Topics