Micromethods in Molecular Biology

1. Front Matter

   Pages I-XV

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2. Micro-Electrophoresis on Polyacrylamide Gels

   • Volker Neuhoff

   Pages 1-83

3. Micro-Determination of Amino Acids and Related Compounds with Dansyl Chloride

   • Volker Neuhoff

   Pages 85-147

4. Micro-Determination of Phospholipids

   • Volker Neuhoff

   Pages 149-178

5. Micro-Diffusion Techniques

   • Volker Neuhoff

   Pages 179-203

6. Capillary Centrifugation

   • Volker Neuhoff

   Pages 205-213

7. Micro-Electrophoresis for RNA and DNA Base Analysis

   • Volker Neuhoff

   Pages 215-256

8. Determination of the Dry Mass of Small Biological Objects by Quantitative Electron Microscopy

   • Volker Neuhoff

   Pages 257-284

9. The Construction and Use of Quartz Fiber Fish Pole Balances

   • Volker Neuhoff

   Pages 285-295

10. Microphotometry

    • Volker Neuhoff

    Pages 297-328

11. Cytofluorometry

    • Volker Neuhoff

    Pages 329-346

12. Quantitative Autoradiography at the Cellular Level

    • Volker Neuhoff

    Pages 347-393

13. Micro-Dialysis

    • Volker Neuhoff

    Pages 395-398

14. Micro-Homogenisation

    • Volker Neuhoff

    Pages 399-402

15. Wet Weight Determination in the Lower Milligram Range

    • Volker Neuhoff

    Pages 403-404

16. Micro-Magnetic Stirrer

    • Volker Neuhoff

    Pages 405-406

17. Production of Capillary Pipettes

    • Volker Neuhoff

    Pages 407-409

18. Back Matter

    Pages 411-430

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This book is based on practical experience and is therefore written as a prac­ tical manual. The fore-runners of the book were the manuals of the first and second EMBO-Courses on "Micromethods in Molecular Biology" which were held in G6ttingen in the spring of 1970 and the autumn of 1971. This book may serve as a manual not only for the participants of the third EMBO-Course to be held in G6ttingen in autumn 1973, but also for all experimenters who are interested in using micromethods. It must be emphasized from the outset that this book is conceived as a "cook book" and not as a monograph which attempts to revue the literature on micromethods critically. The methods described here in detail are performed routinely in the authors' laboratories and include all the practical details necessary for the successful appli­ cation of the micromethods. There are many other sensitive and excellent micro­ methods which are not included in this book, because the authors feel that in a "cook book" only methods for which they have personal experience and profi­ ciency should be described. Some readers may feel that the title promises more than the present contents of this book; however, if sufficient interest is shown in this volume, it may be possible to remedy such deficiencies in future editions.

• DNA
• RNA
• amino acid
• biology
• electron microscopy
• enzyme
• enzymes
• kinetics
• microscopy
• mikroanalyse
• molecular biology
• nucleic acid
• protein
• tRNA
• tissue

• Medizin (Arbeitsgruppe Neurochemie), Max-Planck-Institut für Experimentelle, Göttingen, Germany

  Volker Neuhoff

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