Skip to main content
SpringerLink
Log in

Plant Protoplasts and Genetic Engineering I

  • Book
  • © 1989

Overview

Editors:
  1. Y. P. S. Bajaj

    1. New Delhi, India

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Biotechnology in Agriculture and Forestry (AGRICULTURE, volume 8)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (28 chapters)

  1. Front Matter

    Pages I-XX
    Download chapter PDF

  2. Isolation, Culture, and Preservation of Protoplasts

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Recent Advances in the Isolation and Culture of Protoplasts and Their Implications in Crop Improvement

      • Y. P. S. Bajaj
      Pages 3-22

    3. Enzymes for the Isolation of Protoplasts

      • S. Ishii
      Pages 23-33

    4. Viability of Plant Protoplasts

      • P. B. Gahan
      Pages 34-49

    5. Agarose Plating of Protoplasts and its Applications

      • J. J. M. Dons, C. M. Colijn-Hooymans
      Pages 50-62

    6. Immobilization of Plant Protoplasts

      • H. Schnabl, U. Zimmermann
      Pages 63-96

    7. Cryopreservation of Plant Protoplasts

      • Y. P. S. Bajaj
      Pages 97-106

  3. Regeneration of Plants from Protoplasts

    1. Front Matter

      Pages 107-107
      Download chapter PDF

    2. Regeneration of Plants from Rice Protoplasts

      • J. Kyozuka, K. Shimamoto, H. Ogura
      Pages 109-123

    3. Isolation and Culture of Potato Protoplasts

      • M. Zuba, H. Binding
      Pages 124-146

    4. Isolation and Culture of Citrus Protoplasts

      • Aliza Vardi, Esra Galun
      Pages 147-159

    5. Regeneration of Plants from Linum Protoplasts

      • M. N. Barakat, E. C. Cocking
      Pages 160-172

    6. Isolation and Culture of Glycine Protoplasts

      • C. A. Newell, H. T. Luu
      Pages 173-192

    7. Regeneration of Plants from Cabbage(Brassica oleracea var. capitata) Protoplasts

      • Y. Yamashita, K. Shimamoto
      Pages 193-205

    8. Regeneration of Plants from Chicory (Cichorium intybus L.) Protoplasts

      • Y. Chupeau
      Pages 206-216

    9. Regeneration of Plants from Protoplasts of Lettuce and its Wild Species

      • S. Enomoto, K. Ohyama
      Pages 217-226

    10. Regeneration of Plants from Protoplasts of Butterbur (Petasites japonicus Fr. Schmidt)

      • Kazunori Yabe, Takeshi Nishio, Kenji Takayanagi
      Pages 227-234

    11. Isolation and Culture of Orchid Protoplasts

      • Saburo Yasugi
      Pages 235-253

    12. Regeneration of Plants from Protoplasts of Salpiglossis

      • Charlene J. Boyes, Kenneth C. Sink
      Pages 254-261
Back to top

Keywords

About this book

Isolated protoplasts are a unique tool for genetic manipulation of plants. Since the discovery of a method for the enzymatic isolation of pro-ยท toplasts by Professor E. C. Cocking in 1960, tremendous progress has been made in this very fascinating area of research. I have witnessed the struggle in the 1960's and early 1970's, when obtaining a clean preparaยญ tion of protoplasts was considered an achievement. I also shared the pioneering excitement and enthusiasm in this field during the 2nd Interยญ national Congress of Plant Tissue Culture held at Strasbourg in 1970, where Dr. I. Thkebe of Japan presented his work on the induction of division in tobacco protoplasts. This was followed by my participation in three international conferences devoted to plant protoplasts held in 1972 in Salamanca (Spain) and Versailles (France), and then in 1975 in Nottingham (England). The enthusiasm shown by plant scientists at these meetings was ample proof of the bright future of protoplast technology, and it became evident that protoplasts would playa major role in plant biotechnology, especially in genetic engineering. Since then we have never looked back, and now the methods for isolation, fusion, and culture, as well as regeneration of somatic hybrids, have become routine laboratory procedures for most plant species. Currently the focus is on cereal and tree protoplasts. In order to bring about any genetic manipulation through fusion, inยญ corporation of DNA, and transformation, the regeneration of the entire plant through manipulation of protoplasts is a prerequisite.

Editors and Affiliations

  • New Delhi, India

    Y. P. S. Bajaj

Bibliographic Information

  • Book Title: Plant Protoplasts and Genetic Engineering I

  • Editors: Y. P. S. Bajaj

  • Series Title: Biotechnology in Agriculture and Forestry

  • DOI: https://doi.org/10.1007/978-3-642-73614-8

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 1989

  • Softcover ISBN: 978-3-642-73616-2Published: 08 December 2011

  • eBook ISBN: 978-3-642-73614-8Published: 06 December 2012

  • Series ISSN: 0934-943X

  • Series E-ISSN: 2512-3696

  • Edition Number: 1

  • Number of Pages: XX, 444

  • Topics: Agriculture, Forestry, Plant Sciences, Zoology, Biotechnology

Publish with us

Policies and ethics

Back to top

Search

Navigation