Fluorescence Spectroscopy, Imaging and Probes

1. Front Matter

   Pages I-XXV

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2. Fluorescence Spectroscopy: New Approaches and Probes

   1. Front Matter

      Pages 1-1

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   2. Advanced Luminescent Labels, Probes and Beads and their Application to Luminescence Bioassay and Imaging

      • O. S. Wolfbeis, M. Böhmer, A. Dürkop, J. Enderlein, M. Gruber, I. Klimant et al.

      Pages 3-42

   3. Fluorescence Spectral Engineering — Biophysical and Biomedical Applications

      • J. R. Lakowicz, I. Gryczynski, Y. Shen, J. Malicka, S. D’Auria, Z. Gryczynski

      Pages 43-68

   4. Fluorescence Nanometrology in Sol-Gels

      • D. J. S. Birch, C. D. Geddes, J. Karolin, R. Leishman, O. J. Rolinski

      Pages 69-85

   5. Integrated Supramolecular Systems: From Sensors to Switches

      • J.-P. Malval, I. Gosse, J.-P. Morand, R. Lapouyade

      Pages 87-100

   6. Ratiometric Probes: Design and Applications

      • A. P. Demchenko, A. S. Klymchenko, V. G. Pivovarenko, S. Ercelen

      Pages 101-110

   7. Binding of Ethidium to Yeast tRNAPhe: A New Perspective on an Old Bromide

      • M. Tramier, O. Holub, J. C. Croney, T. Ishi, S. E. Seifried, D. M. Jameson

      Pages 111-121

   8. Experimental Aspects of DNA Computing by Blocking: Use of Fluorescence Techniques for Detection

      • K. A. Schmidt, C. v. Henkel, G. Rozenberg, H. P. Spaink

      Pages 123-128

3. Fluorescence Spectroscopy of Single Molecules and Molecular Assemblies

   1. Front Matter

      Pages 129-129

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   2. Multiparametric Detection of Fluorescence Emitted from Individual Multichromophoric Systems

      • M. Cotlet, J. Hofkens, M. Maus, F. C. de Schryver

      Pages 131-151

   3. Fluorescence Intensity Distribution Analysis (FIDA) and related fluorescence fluctuation techniques: theory and practice

      • P. Kask, C. Eggeling, K. Palo, Ü. Mets, M. Cole, K. Gall

      Pages 153-181

   4. Single Molecule Reactions of the Enzyme LDH and of Restriction Endonucleases in the Fluorescence Microscope

      • B. Nasanshargal, B. Schäfer, K. O. Greulich

      Pages 183-195

   5. Monitoring γ-Subunit Movement in Reconstituted Single EF°F1 ATP Synthase by Fluorescence Resonance Energy Transfer

      • M. Börsch, M. Diez, B. Zimmermann, R. Reuter, P. Gräber

      Pages 197-207

4. Application of Fluorescence in Biological Membrane and Enzyme Studies

   1. Front Matter

      Pages 209-209

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   2. Application of the Wavelength-selective Fluorescence Approach to Monitor Membrane Organization and Dynamics

      • A. Chattopadhyay

      Pages 211-224

   3. Fluorescence Approaches for the Characterization of the Peripheral Membrane Binding of Proteins Applied for the Blood Coagulation Protein Prothrombin

      • R. Hutterer, M. Hof

      Pages 225-240

   4. Assessment of Membrane Fluidity in Individual Yeast Cells by Laurdan Generalised Polarisation and Multi-photon Scanning Fluorescence Microscopy

      • R. P. Learmonth, E. Gratton

      Pages 241-252

   5. Formation of Higher Order Signal Transduction Complexes as Seen by Fluorescence Spectroscopy

      • L. Dowal, S. Scarlata

      Pages 253-261

   6. Mechanisms of the Modulation of Membrane Interfacial Enzyme Catalysis by Non-lamellar Forming Lipids: Comparison with the Behavior of a Fluorescent Probe in Membranes

      • R. M. Epand, R. Cornell, S. M. A. Davies, R. Kraayenhof

      Pages 263-276

The application of fluorescence in drug discovery, high-throughout screenings in genomics and proteomics is and will be evidently successful. The increased use of fluorescence techniques is greatly enhanced by the improved instrumentation pioneered by inventive scientists and now made available commercially by several high-tech companies. Moreover, the design and development of many new molecular probes with higher selectivity for specific microenvironmental properties has stimulated many new researchers to employ fluorescence techniques for solving their problems. Probably the most significant breakthrough in fluorescence is its use in detection of single molecules and even of their real-time dynamics. Also, probing inside living cells has become a hot topic in the life sciences. This topic book reflects the updates of scientific progress presented by frontline researchers.

• DNA
• Galvanometer
• Polarisation
• Purine
• biology
• cell culture
• enzymes
• imaging
• metrology
• microscopy
• nanometrology
• scaling
• sensor
• sensors
• spectroscopy

• Institute of Molecular Biological Sciences, Amsterdam, Vrije Universiteit, Amsterdam, The Netherlands

  Ruud Kraayenhof

• MicroSpectroscopy Center, Wageningen University, Wageningen, The Netherlands

  Antonie J. W. G. Visser

• Dept. of Molecular Biophysics, University of Utrecht, Utrecht, The Netherlands

  Hans C. Gerritsen

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