Gene Technology

1. Front Matter

   Pages I-XII

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2. Stem Cells

   1. Front Matter

      Pages 1-1

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   2. Hematopoietic Differentiation of Embryonal Stem Cells in vitro.

      • Nicholas Hole, Roland Leung, Lobat Doostdar, Ursula Menzel, Kay Samuel, Janice Murray et al.

      Pages 3-10

   3. Post-irradiated canine serum (PICS-J) stimulates megakaryocyte (MK) progenitors and thrombopoiesis in-vitro

      • Varda R. Deutsch, Amiram Eldor, Tom Olson, Shimon Slavin, Arnon Nagler

      Pages 11-27

   4. The Adhesion Molecules, PECAM-1, ICAM-3 and CD33, on CD34+ Haematopoietic Progenitor Cells

      • Suzanne M. Watt, Angelo Cardoso, Jane Lee-Prudhoe, M. Ann Benton, Regis Doyonnas, Antoinette Hatzfeld et al.

      Pages 29-76

   5. Hematopoietic stem cell: developmental potential and proliferative activity

      • J.L Chertkov, N.J Drize, O.I Gan, N.I Olovnikova, E.V Belkina, T.L Nikolaeva

      Pages 77-90

   6. The Effect of Methylprednisolone on Bone Marrow and Spleen Clonogenic Fibroblasts in Humans

      • E. Vladimirskaya, N. Zamaraeva, E. Osipova, M. Volunetz, I. Alexeev, O. Kryzianovsky et al.

      Pages 91-97

   7. Umbilical cord blood: Morphological composition and functional characteristics

      • I. Alexeev, M. Volynetz, N. Zamaraeva, E. Osipova, A. Loginov, E. Vladimirskaya et al.

      Pages 99-106

   8. Stroma cell dependent leukemias Growth of PGM-1 leukemia cells on stromal layers

      • Ulrich Dýhrsen

      Pages 107-116

3. Genes Crucial for Development and Disease

   1. Front Matter

      Pages 117-117

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   2. The Role of Non-receptor Protein Tyrosine Kinases in Receptor Mediated Cellular Activation and Immunodeficiency Disease

      • Sandra J. Saouaf, Anne L. Burkhardt, Joseph B. Bolen

      Pages 119-134

   3. Directed Gene Trapping in Embryonic Stem Cells

      • L.M Forrester, A. Joyner, A. Bernstein, W. Wurst

      Pages 135-144

   4. Molecular Basis of Genetic Disorders of Pigmentation in Humans and Mice

      • Richard A. Spritz

      Pages 145-174

   5. MDR-1 Gene Expression in Chronic Myelogenous Leukemia: Prognostic Marker of the Disease

      • I. Stuif, A. Sominskaya, T. Bykova, O. Frolova, A. Zaritskey

      Pages 175-179

4. Methods of Gene Transfer, Applications for Gene Therapy

   1. Front Matter

      Pages 181-181

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   2. Towards Gene Therapy for Haemophilia B

      • Ann Gerrard

      Pages 183-188

   3. Inhibition of autocrine TGF-ß in CD34+ human progenitor cells reveals their potentiality to engraft adults and improves gene transfer efficiency

      • Jacques Hatzfeld, Béatrice Panterne, Pascal Batard, Patricia Sansilvestri, Ma-Lin Li, Angelo A. Cardoso et al.

      Pages 189-192

   4. Gene Transfer into Hemopoietic Progenitors: Implications for Bone Marrow Transplantation

      • Helen E. Heslop, Cliona M. Rooney, Donna R. Rill, Malcolm K. Brenner

      Pages 193-202

   5. Erythropoietin and the ENV gp55 of the Spleen Focus Forming Virus (SFFV) Interact Differently with Erythroid Cells in the Mouse and in Tissue Culture

      • Ursula Just, Norbert Ahlers, Gökhan Arman, Nicholas Hunt, Wolfram Ostertag, Joachim Nowock

      Pages 203-218

   6. Contribution of the Retroviral Leader to Gene Transfer and Expression in Packaging Cells and Myeloid Stem and Progenitor Cells

      • Christopher Baum, Hans-Georg Eckert, Wolfram Ostertag

      Pages 219-228

The 11 th meeting in "Modern Trends in Human Leukemia" took place from June 19 to 21, 1994 in Wilsede in the middle of the Liineburger Heide, South of Hamburg. Interwoven with the Leukemia program was the Nato-sponsored Symposium of the ASI-Series "Gene Technology in Analysis of Malignant and Inherited Human Diseases Related to Development" . The Wilsede meeting was continued on a ship of the Neva leading through lake Ladoga and lake Onega. The topics of both meetings included discussion on recent progress isolation and development of hematopoietic stem cells, genes crucial for development and diseases, methods of gene transfer, application of gene transfer; oncogenes and anti-oncogenes as targets for gene therapy; receptors and their ligands in normal development and diseases, immunology and immunotherapy, radiation biology, clinical leukemias and bone marrow transplantation. The Nato workshop concentrated not only on analysis of cell systems useful for somatic gene therapy, but also on actual themes directly related to correction of human diseases. The latter aspects emphasized themes related to biotechnology, the first part was by nature more general. We also included a few contributions that discussed perspectives for the future of gene therapy and possible relationships to evolution.

• Antionkogene
• Gentransfer
• Histokompatibilitätsantigene
• Knochenmarkstransplantation
• Stammzellen
• bone marrow transplantation
• gene therapy
• gene transfer
• genes
• histocompatibility antigenes
• transplantation

• Universitätskrankenhaus Eppendorf, Hamburg, Germany

  Axel R. Zander

• Heinrich-Pette-lnstitut für Experimentelle Virologie und Immunologie, Universität Hamburg, Hamburg, Germany

  Wolfram Ostertag

• City Center for Advanced Medical Technologies, Bone Marrow Transplantation Center, St.Petersburg, Russia

  Boris V. Afanasiev

• Laboratory for Gene Structure Expression, Medical Research Council Institute, London, UK

  Frank Grosveld

Policies and ethics