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Techniques in Molecular Medicine

  • Book
  • © 1999

Overview

Editors:
  1. Friedhelm Hildebrandt

    1. Universitäts-Kinderklinik, Freiburg, Germany

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    You can also search for this editor in PubMed   Google Scholar

  2. Peter Igarashi

    1. Section of Nephrology, Dep. of Internal Medicine, Yale University, New Haven, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Designed for researchers who are just starting or planning to use molecular biology techniques in clinical laboratories The manual provides well-established basic protocols with detailed instructions serves as a working tool and also as a short, illustrated compendium of molecular biology
  • Includes supplementary material: sn.pub/extras

Part of the book series: Springer Lab Manuals (SLM)

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Table of contents (24 chapters)

  1. Front Matter

    Pages I-XII
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  2. Molecular Biology Techniques

    1. Front Matter

      Pages 1-1
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    2. Structure and Function of the Human Genome

      • Friedhelm Hildebrandt
      Pages 3-23

    3. Enzymatic Modification of DNA and RNA

      • Kai-Olaf Netzer
      Pages 24-49

    4. Purification of Nucleic Acids From Eukaryotic Cells

      • Hans Gerd Nothwang, Friedhelm Hildebrandt
      Pages 50-70

    5. Electrophoresis of DNA and RNA Fragments

      • Feng Qian, Gregory G. Germino
      Pages 71-81

    6. Single-Strand Conformation Polymorphism (SSCP) Analysis

      • Feng Qian, Gregory G. Germino
      Pages 82-85

    7. Denaturing Gradient Gel Electrophoresis

      • Kai-Olaf Netzer
      Pages 86-95

    8. Introduction to Pulsed-Field Gel Electrophoresis (PFGE)

      • Feng Qian, Gregory G. Germino
      Pages 96-102

    9. Pulsed-Field Gel Electrophoresis: Protocols

      • Gudrun A. Rappold, Karin Ried, Albrecht Klink, Ercole Rao, Birgit Weiss
      Pages 103-113

    10. Radioactive Labeling of DNA and RNA Probes

      • Peter Igarashi
      Pages 114-125

    11. Hybridization Methods (Southern and Northern Blotting)

      • Kai-Olaf Netzer
      Pages 126-147

    12. Specimen Preparation Techniques for Cytogenetic Analysis by Fluorescence in situ Hybridization

      • Stefan Joos, Martin Bentz, Anton H. N. Hopman, Peter Lichter
      Pages 148-165

    13. Chromosome Analysis by Fluorescence in situ Hybridization

      • Martin Bentz, Stefan Joos, Peter Lichter
      Pages 166-185

    14. Sequencing

      • Klaus Deichmann
      Pages 186-206

    15. Polymerase Chain Reaction

      • Friedhelm Hildebrandt, Iva Singh-Sawhney
      Pages 207-225

    16. Cloning Vectors

      • Annette Deichmann, Klaus Deichmann
      Pages 226-254

  3. Strategies for Gene Isolation and Characterization

    1. Front Matter

      Pages 255-255
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    2. Cloning Strategies: An Overview

      • Friedhelm Hildebrandt
      Pages 257-260

    3. Genomic Libraries

      • Luiz F. Onuchic, Gregory G. Germino
      Pages 261-272
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Keywords

About this book

Standard molecular biology techniques are used widely in biomedical re­ search and have had a major impact on the understanding of physiologic processes and disease mechanisms. They also play an increasing role in mo­ lecular genetic diagnosis and new therapeutic approaches. This laboratory manual is designed for researchers who need reliable pro­ tocols for their laboratorywork. It provides a step by step approach to stan­ dard methods of molecular biology. A wide range of topics is covered by protocols for immediate application in the laboratory. These include pur­ ification, isolation, labeling, hybridization, sequencing and enzymatic mod­ ification ofDNA or RNA, polymerase chain reaction, cloning, libraries, and protein techniques. The manual is based on experience with a practical course in molecular biologytechniques, which was taught bythe authors. Since most methods in molecular biology recapitulate in a test tube mechanisms that occur natu­ rally in the cell, the first three chapters describe and illustrate basic mechan­ isms of molecular biology. U nderstanding more about these basic mechan­ isms will enable the researcher to apply techniques more specifically, based on the knowledge of their potential and limits. W e hope that users find this manual a helpful guide on their road to exciting insights.

Editors and Affiliations

  • Universitäts-Kinderklinik, Freiburg, Germany

    Friedhelm Hildebrandt

  • Section of Nephrology, Dep. of Internal Medicine, Yale University, New Haven, USA

    Peter Igarashi

Bibliographic Information

  • Book Title: Techniques in Molecular Medicine

  • Editors: Friedhelm Hildebrandt, Peter Igarashi

  • Series Title: Springer Lab Manuals

  • DOI: https://doi.org/10.1007/978-3-642-59811-1

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 1999

  • Softcover ISBN: 978-3-642-47808-6Published: 23 August 2014

  • eBook ISBN: 978-3-642-59811-1Published: 11 November 2013

  • Edition Number: 1

  • Number of Pages: XII, 387

  • Number of Illustrations: 48 b/w illustrations, 3 illustrations in colour

  • Topics: Molecular Medicine, Cell Biology, Human Genetics

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