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Proteomic Methods in Neuropsychiatric Research

  • Book
  • © 2017

Overview

Editors:
  1. Paul C. Guest

    1. Department of Biochemistry and Tissue Biology, University of Campinas (UNICAMP), Campinas, Brazil

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  • Presents several strategy based chapters, such as those that explain the importance of proteomics in translational medicine and the need for increased proteomic-based platforms in the drug discovery pipeline
  • Describes step-by-step instructions on how to carry out each of the protocols
  • Looks at the future of proteomic platforms including had held devices

Part of the book series: Advances in Experimental Medicine and Biology (AEMB, volume 974)

Part of the book sub series: Proteomics, Metabolomics, Interactomics and Systems Biology (PMISB)

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Table of contents (35 chapters)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Reviews

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Application of Proteomic Techniques for Improved Stratification and Treatment of Schizophrenia Patients

      • Johann Steiner, Paul C. Guest, Daniel Martins-de-Souza
      Pages 3-19

    3. Multiplexing Biomarker Methods, Proteomics and Considerations for Alzheimer’s Disease

      • Renã A. S. Robinson, Bushra Amin, Paul C. Guest
      Pages 21-48

    4. What Have Proteomic Studies Taught Us About Novel Drug Targets in Autism?

      • Paul C. Guest, Daniel Martins-de-Souza
      Pages 49-67

    5. Application of Proteomic Approaches to Accelerate Drug Development for Psychiatric Disorders

      • Hassan Rahmoune, Daniel Martins-de-Souza, Paul C. Guest
      Pages 69-84

    6. Proteomic Biomarker Identification in Cerebrospinal Fluid for Leptomeningeal Metastases with Neurological Complications

      • Norma Galicia, Paula Díez, Rosa M. Dégano, Paul C. Guest, Nieves Ibarrola, Manuel Fuentes
      Pages 85-96

    7. Connecting Brain Proteomics with Behavioural Neuroscience in Translational Animal Models of Neuropsychiatric Disorders

      • Zoltán Sarnyai, Paul C. Guest
      Pages 97-114

    8. LC-MSE for Qualitative and Quantitative Proteomic Studies of Psychiatric Disorders

      • Mariana Fioramonte, Paul C. Guest, Daniel Martins-de-Souza
      Pages 115-129

    9. The Utility of Multiplex Assays for Identification of Proteomic Signatures in Psychiatry

      • Junfang Chen, Paul C. Guest, Emanuel Schwarz
      Pages 131-138

  3. Protocols

    1. Front Matter

      Pages 139-139
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    2. Blood Sampling and Preparation Procedures for Proteomic Biomarker Studies of Psychiatric Disorders

      • Paul C. Guest, Hassan Rahmoune
      Pages 141-147

    3. Multiplex Immunoassay Profiling of Serum in Psychiatric Disorders

      • Laurie Stephen, Emanuel Schwarz, Paul C. Guest
      Pages 149-156

    4. Sequential Immunoprecipitation of Secretory Vesicle Proteins from Biosynthetically Labelled Cells

      • Paul C. Guest
      Pages 157-165

    5. 2D Gel Electrophoresis of Insulin Secretory Granule Proteins from Biosynthetically Labelled Pancreatic Islets

      • Paul C. Guest
      Pages 167-174

    6. Two-Dimensional Gel Electrophoresis: A Reference Protocol

      • Veronica M. Saia-Cereda, Adriano Aquino, Paul C. Guest, Daniel Martins-de-Souza
      Pages 175-182

    7. A Two-Dimensional Difference Gel Electrophoresis (2D-DIGE) Protocol for Studies of Neural Precursor Cells

      • Paul C. Guest
      Pages 183-191

    8. Identifying Biomarker Candidates in the Blood Plasma or Serum Proteome

      • Sheila Garcia, Licia C. Silva-Costa, Guilherme Reis-de-Oliveira, Paul C. Guest, Paulo A. Baldasso, Juliana S. Cassoli et al.
      Pages 193-203

    9. Selective Reaction Monitoring Mass Spectrometry for Quantitation of Glycolytic Enzymes in Postmortem Brain Samples

      • Guilherme Lanfredi, Guiherme Reis-de-Oliveira, Veronica M. Saia-Cereda, Paul C. Guest, Daniel Martins-de-Souza, Vitor M. Faça
      Pages 205-212

    10. A Selected Reaction Monitoring Mass Spectrometry Protocol for Validation of Proteomic Biomarker Candidates in Studies of Psychiatric Disorders

      • Guilherme Reis-de-Oliveira, Sheila Garcia, Paul C. Guest, Juliana S. Cassoli, Daniel Martins-de-Souza
      Pages 213-218
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Keywords

About this book

Due to continuous technical developments and new insights into the high complexity of neurological diseases, there is an increasing need for the application of proteomic technologies which can yield potential biomarker readouts for improved clinical management as well as for the development of new drugs by struggling pharmaceutical companies. This book describes the step-by-step use of proteomic methods such as two-dimensional gel electrophoresis, multiplex immunoassay, liquid chromatography mass spectrometry (LC-MS) and selective reaction monitoring MS, to increase our understanding of these diseases, with the ultimate aim of improving patient care.

The volume will be of high interest to clinical scientists, physicians and pharmaceutical company scientists as it gives insights into the latest technologies enabling the revolution of personalized medicine.  It is of direct interest to both technical and bench biomarker scientists as it gives step by step instructions on how to carry out each of the protocols. It is also  of interest to researchers as each technique will be presented in the context of a specific neurological disorder, including Alzheimer's disease, multiple sclerosis, autism spectrum disorders, schizophrenia, major depressive disorder and bipolar disorder. 


Finally, it will also highlight the future research efforts in this field, which are endeavoring to convert proteomic platforms to the form of hand held devices which can be used in a point of care setting and return diagnostic results within the timeframe of a visit to the general practitioner.

Editors and Affiliations

  • Department of Biochemistry and Tissue Biology, University of Campinas (UNICAMP), Campinas, Brazil

    Paul C. Guest

Bibliographic Information

  • Book Title: Proteomic Methods in Neuropsychiatric Research

  • Editors: Paul C. Guest

  • Series Title: Advances in Experimental Medicine and Biology

  • DOI: https://doi.org/10.1007/978-3-319-52479-5

  • Publisher: Springer Cham

  • eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)

  • Copyright Information: Springer International Publishing AG 2017

  • Hardcover ISBN: 978-3-319-52478-8Published: 04 April 2017

  • Softcover ISBN: 978-3-319-84911-9Published: 21 July 2018

  • eBook ISBN: 978-3-319-52479-5Published: 28 March 2017

  • Series ISSN: 0065-2598

  • Series E-ISSN: 2214-8019

  • Edition Number: 1

  • Number of Pages: XII, 370

  • Number of Illustrations: 10 b/w illustrations, 45 illustrations in colour

  • Topics: Proteomics, Gene Expression, Gene Therapy

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