Stem Cell Processing

1. Front Matter

   Pages i-xiii

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2. Stem Cell Therapy: Accepted Therapies, Managing the Hope of Society, and a Legal Perspective

   • W. M. Botes, M. Nöthling Slabbert, M. Alessandrini, M. S. Pepper

   Pages 1-35

3. Mesenchymal Stem Cells in Clinical Applications

   • Phuc Van Pham

   Pages 37-69

4. Ageing and Senescence in Mesenchymal Stem Cells

   • Hitesh D. Tailor, Yiannis Pengas, Wasim S. Khan

   Pages 71-75

5. New Trends in Clinical Applications of Induced Pluripotent Stem Cells

   • Phuc Van Pham, Nhan Lu-Chinh Phan, Ngoc Bich Vu, Nhung Hai Truong, Ngoc Kim Phan

   Pages 77-98

6. The Effects of Ageing on Proliferation Potential, Differentiation Potential and Cell Surface Characterisation of Human Mesenchymal Stem Cells

   • Emma Fossett, Yiannis Pengas, Wasim S. Khan

   Pages 99-106

7. Production of Clinical-Grade Mesenchymal Stem Cells

   • Phuc Van Pham, Ngoc Bich Vu

   Pages 107-129

8. Isolation and Characterization of Adipose-Derived Stromal Cells

   • Fiona A. van Vollenstee, Carla Dessels, Karlien Kallmeyer, Danielle de Villiers, Marnie Potgieter, Chrisna Durandt et al.

   Pages 131-161

9. Cord Blood Stem Cell Banking

   • Helen C. Steel, Marco Alessandrini, Juanita Mellet, Carla Dessels, Ahmed K. Oloyo, Michael S. Pepper

   Pages 163-180

10. Human Embryonic Stem Cells and Associated Clinical Concerns

    • Deepak M. Kalaskar, Saiyyada Mohsina Shahid

    Pages 181-197

11. Harvesting and Collection of Adipose Tissue for the Isolation of Adipose-Derived Stromal/Stem Cells

    • Fiona A. van Vollenstee, Danie Hoffmann, Michael S. Pepper

    Pages 199-220

12. Back Matter

    Pages 221-225

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This invaluable resource delineates procedures for development and use of stem cells in the laboratory and explores the potential for clinical applications. The text discusses mesenchymal stem cell isolation, isolation of adipose derived stem cells, new trends of induced pluripotent stem cells in disease treatment, cord blood banking, future directions of the discussed therapies and much more. The chapters are contributed by preeminent scientists in the field and present a comprehensive picture of stem cell processes, from development in the laboratory to effects and side-effects of clinical application.


Stem Cell Processing and the other books in the Stem Cells in Clinical Applications series, edited by Dr. Phuc Van Pham, is essential reading for scientists, researchers, advanced students and clinicians working in stem cells, regenerative medicine or tissue engineering.

• adipose tissue
• mesenchymal stem cells
• musculoskeletal regeneration
• osteoarthritis
• stromal cells

• Laboratory of Stem Cell Research and Application, University of Science Vietnam National University, Ho Chi Minh City, Vietnam

  Phuc Van Pham

Phuc Van Pham received his Ph.D. in Human Physiology from Vietnam National University, Ho Chi Minh City, Vietnam. He is currently a Professor of Biology at Vietnam National University and Acting Director of the Laboratory of Stem Cell Research and Application. He is a longstanding lecturer and translational scientist at the University, and is a member of several societies and journal editorial boards focused on stem cells.


Dr. Pham and his colleagues have established one of the first multidisciplinary stem cell centers in Vietnam, and he has successfully launched an array of technologies in stem cell isolations. His research interests include stem cell isolation, stem cell therapy, mesenchymal stem cells, cancer stem cells, immunotherapy and regenerative medicine and he has published extensively in these areas.


After many years of experience as an embryologist, cell biologist, and molecular biologist, collaborating with leading researchers in Singapore, Japan, and the United States, Dr. Pham is a student again, keen to reach beyond the traditional boundaries of biology.

Policies and ethics