Overview
- A comprehensive review
- Written by experts
- Richly illustrated
- Includes supplementary material: sn.pub/extras
Part of the book series: Springer Series on Fluorescence (SS FLUOR, volume 15)
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Table of contents (16 chapters)
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Front Matter
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Back Matter
Keywords
About this book
This volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a “fingerprint” for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of Förster Resonant Energy Transfer (FRET) for the investigation of protein folding and interaction. Several technology-related chapters present both the basics and current state-of-the-art, in particular of TCSPC electronics, photon detectors and lasers. The remaining chapters cover a broad range of applications and methodologies for experiments and data analysis, including the life sciences, defect centers in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new options arising from the combination of classic TCSPC and fluorescence lifetime with methods based on intensity fluctuation represent a particularly unique highlight.
Reviews
“The book is quite specialised and will likely be mainly of interest to researchers developing new photon counting techniques, fluorescence-based or not. … In addition to the constructors of photon counting systems, it could also be useful to some as a reference for comparing results, which could include those working in the life sciences as well as physicists and chemists.” (David Birch, Analytical and Bioanalytical Chemistry, Vol. 408, 2016)
Editors and Affiliations
About the editors
Bibliographic Information
Book Title: Advanced Photon Counting
Book Subtitle: Applications, Methods, Instrumentation
Editors: Peter Kapusta, Michael Wahl, Rainer Erdmann
Series Title: Springer Series on Fluorescence
DOI: https://doi.org/10.1007/978-3-319-15636-1
Publisher: Springer Cham
eBook Packages: Chemistry and Materials Science, Chemistry and Material Science (R0)
Copyright Information: Springer International Publishing Switzerland 2015
Hardcover ISBN: 978-3-319-15635-4Published: 06 May 2015
Softcover ISBN: 978-3-319-35429-3Published: 13 October 2016
eBook ISBN: 978-3-319-15636-1Published: 23 April 2015
Series ISSN: 1617-1306
Series E-ISSN: 1865-1313
Edition Number: 1
Number of Pages: XII, 370
Number of Illustrations: 56 b/w illustrations, 103 illustrations in colour
Topics: Spectroscopy/Spectrometry, Optics, Lasers, Photonics, Optical Devices, Biological and Medical Physics, Biophysics