Methods in Protein Sequence Analysis

1. Front Matter

   Pages I-8

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2. Sequencer Methodology and Instrumentation

   1. Modular Berlin Microsequencer for the Sequential Degradation of Proteins and Peptides from the Amino- and Carboxyl-Terminal End

      • Brigitte Wittmann-Liebold, Lothar Matschull, Ulrike Pilling, Hans-Arthur Bradaczek, Horst Graffunder

      Pages 9-21

   2. C-Terminal Sequence Analysis

      • Adam S. Inglis, Robert L. Moritz, Geoffrey S. Begg, Gavin E. Reid, Richard J. Simpson, Horst Graffunder et al.

      Pages 23-34

   3. Chemical C-Terminal Sequencing

      • David H. Hawke, Victoria L. Boyd

      Pages 35-45

   4. Extending the Performance of the Solid-Phase Protein Sequencer

      • Richard A. Laursen, Thomas T. Lee, James D. Dixon, Song-Ping Liang

      Pages 47-54

   5. Direct Microsequencing of Blotted and Covalently Attached Proteins in a Cross-Flow Reaction Chamber

      • H. Reinke, St. Fischer, F. Reimann, H. Tschesche

      Pages 55-66

3. Sample Preparation and Analysis

   1. Current Strategies for Microscale Purification of Protein and Peptides for Sequence Analysis

      • Richard J. Simpson, Robert L. Moritz, Gavin E. Reid, Larry D. Ward

      Pages 67-77

   2. Capillary Electrophoresis: A New Dimension in the Separation Sciences

      • T. Large

      Pages 79-90

   3. Structural Analysis of Membrane Proteins

      • John E. Shively

      Pages 91-101

   4. Protein-Electroblotting and Microsequencing in Establishing Integrated Human Protein Databases

      • H. H. Rasmussen, J. Van Damme, G. Bauw, M. Puype, B. Gesser, J. E. Celis et al.

      Pages 103-114

   5. Comparison of the Blotting Efficiencies of Various PVDF Membranes

      • Paul Matsudaira

      Pages 115-121

   6. Sensitive Determination of Amino Acid Derivatives from N-Terminal Sequence Analysis

      • A. Tsugita, M. Kamo

      Pages 123-132

   7. Amino Acid Analysis and Sequencing — What is State-of-the-Art?

      • Ronald L. Niece, Lowell H. Ericsson, Audree V. Fowler, Alan J. Smith, David W. Speicher, John W. Crabb et al.

      Pages 133-141

   8. Realistic Expectations for Amino Acid Analysis

      • L. H. Ericsson, D. Atherton, R. Kutny, A. J. Smith, J. W. Crabb

      Pages 143-150

4. Modified Residues, Chemical Problems and Synthetic Peptides

   1. A Protein Chemistry Approach to the Modelling of Integral Membrane Proteins

      • John B. C. Findlay

      Pages 151-160

   2. N-Terminal Acetylated Mitochondrial Aldehyde Dehydrogenase is Found in Fresh but not Frozen Liver Tissue

      • H. Weiner, S. C. Cunningham, R. A. Angelo

      Pages 161-167

   3. Elucidating Ligand Binding Sites in Polypeptides by Photoaffinity Labeling with Aryl Azides

      • H. Ponstingl, L. D. Barnes, C. Granzow, R. H. Himes, G. Maier, G. Nasioulas

      Pages 169-176

   4. Use of Thiopropyl-Sepharose 6B for Isolation and Structure-Functional Analysis of Thiol Proteins

      • Ts. A. Egorov

      Pages 177-185

   5. Zinc Fingers Involved in MHC Class I Gene Regulation: Use of Synthetic Peptides for Structural Analysis

      • E. Appella, J. G. Omichinski, G. M. Clore, A. M. Gronenborn, K. Sakaguchi

      Pages 187-195

   6. Hydrophobic Surfactant Proteins SP-B and SP-C: Special Analytical Problems

      • Jan Johansson, Tore Curstedt, Per Persson, Bengt Robertson, Björn Löwenadler, Hans Jörnvall

      Pages 197-204

Methods in protein sequence analysis constitute important fields in rapid progress. We have experienced a continuous increase in analytical sensitivity coupled with decreases in time necessary for purification and analysis. Several generations of sequencers, liquid/solid/gas-phase, have passed by and returned in other shapes during just over two decades. Similarly, the introduction of HPLC permitted an enormous leap forward in this as in other fields of biochemistry, and we now start to see new major advances in purification/analysis through capillary electrophoresis. Furthermore, progress in the field of mass spectrometry has matched that in chemical analysis and we witness continuous development, now emphasizing ion spray and other mass spectrometric approaches. In short, protein analysis has progressed in line with other developments in modern science and constitutes an indispensable, integral part of present-day molecular biology. Even the available molecular tools, in the form of proteases with different specificities, have increased in number, although we still have far to go to reach an array of "restriction proteases" like the sets of nucleases available to the molecular geneticist. Of course, conferences have been devoted to protein sequence analysis, in particular the MPSA (Methods in Protein Sequence Analysis) series, of which the 8th conference took place in Kiruna, Sweden, July 1-6 1990. Again, we witnessed much progress, saw new instruments, and experienced further interpretational insights into protein mechanisms and functions.

• membrane proteins
• protein
• protein sequence
• Secondary structure
• tertiary structure

• Department of Chemistry I, Karolinska Institute, Stockholm, Sweden

  Hans Jörnvall, Jan-Olov Höög, Ann-Margreth Gustavsson

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