Soft Probes for Bio-electrochemical Imaging

1. Front Matter

   Pages i-xxi

   PDF

2. Introduction

   • Tzu-En Lin

   Pages 1-24

3. Experimental Part

   • Tzu-En Lin

   Pages 25-36

4. Multiple SECM Mapping of Tyrosinase in Micro-contact Printed Fruit Samples on Polyvinylidene Fluoride Membrane

   • Tzu-En Lin

   Pages 37-52

5. Monitoring Tyrosinase Expression in Non-metastatic and Metastatic Melanoma Tissues by SECM

   • Tzu-En Lin

   Pages 53-62

6. From Tissues to Organs: Soft Electrochemical Probes for Scanning Human and Animal Tissues

   • Tzu-En Lin

   Pages 63-85

7. Imaging the Distribution of Graphene Oxide Nanoribbons in Mice Livers by Soft Probe SECM

   • Tzu-En Lin

   Pages 87-100

8. Mapping the Antioxidant Activity of Apple Peels with Soft Probe Scanning Electrochemical Microscopy

   • Tzu-En Lin

   Pages 101-116

9. General Conclusions and Future Perspectives

   • Tzu-En Lin

   Pages 117-120

This book discusses the development of various reliable scanning electrochemical microscopy (SECM) imaging techniques for studying the distribution of biomarkers and nanomaterials in thin and thick animal samples, plant antioxidant (AO) defense systems, as well as human melanoma. The authors demonstrate that SECM could improve the diagnosis and understanding of different melanoma stages on the basis of highly resolved maps of the tyrosinase distribution. Tyrosinase is the key enzyme involved in fruit maturation and is a biomarker for melanoma. As such the book presents various tyrosinase SECM detection strategies developed for the analysis of the spatial distribution of tyrosinase in melanoma and in banana samples. It describes the first imaging of the redox active proteins within the entire mouse heart with an SECM system using a spider probe composed of eight independent microelectrodes. Further, it investigates distributions of injected graphene nanoribbons (GONRs) for drug deliveryby Soft-Probe-SECM. Lastly, the book outlines a non-invasive electrochemical strategy for mapping the AO activity of apple peel using Soft-Probe-SECM.

• Scanning Electrochemical Microscopy (SECM)
• Microelectrode
• Soft Probe
• Spider Probe
• Tyrosinase
• Melanoma
• Graphene Oxide Nanoribbon
• Thick Tissue
• Contact Mode Scanning
• Feedback Mode
• Generation Collection Mode
• Bioimaging
• Biosensing

• Chemistry and Chemical Engineering, École Polytechnique Fédérale de Lausanne, Sion, Switzerland

  Tzu-En Lin

Dr. Tzu-En Lin is currently a postdoctoral researcher with a specialization in electrochemistry at École Polytechnique Fédérale de Lausanne, Switzerland (EPFL). She obtained her PhD degree from EPFL and her master’s degree from National Taiwan University. Her supervisor is Prof. Hubert Girault, an expert in physical and analytical chemistry, battery technology and hydrogen car development. She was admitted to the US “Stanford University-EPFL exchange program” as a visiting scholar. Her work on “banana and skin cancer” has been reported worldwide. 

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