Skip to main content
  • Book
  • © 2014

Protein Downstream Processing

Design, Development and Application of High and Low-Resolution Methods

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1129)

Buy it now

Buying options

eBook USD 119.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, log in via an institution to check for access.

Table of contents (38 protocols)

  1. Front Matter

    Pages i-xv
  2. Screening and Design Purification Strategies

    1. Front Matter

      Pages 1-1
    2. Protein Purification: An Overview

      • Nikolaos E. Labrou
      Pages 3-10
    3. Establishment of a Design Space for Biopharmaceutical Purification Processes Using DoE

      • Ignacio Amadeo, Laura Mauro, Eduardo Ortí, Guillermina Forno
      Pages 11-27
    4. High-Throughput Process Development: I. Process Chromatography

      • Anurag S. Rathore, Rahul Bhambure
      Pages 29-37
    5. High-Throughput Process Development: II. Membrane Chromatography

      • Anurag S. Rathore, Sampath Muthukumar
      Pages 39-44
    6. Media Selection in Ion-Exchange Chromatography in a Single Microplate

      • Charlotte Cabanne, Xavier Santarelli
      Pages 45-51
    7. High-Throughput Screening of Dye-Ligands for Chromatography

      • Sunil Kumar, Narayan S. Punekar
      Pages 53-65
  3. High-Resolution Protein Purification Methods

    1. Front Matter

      Pages 145-145
    2. Affinity Tags in Protein Purification and Peptide Enrichment: An Overview

      • Ana Sofia Pina, Íris L. Batalha, Ana Cecília A. Roque
      Pages 147-168
    3. Expression and Purification of Large Active GST Fusion Enzymes

      • Stefania Deceglie, Claudia Lionetti, Marina Roberti, Palmiro Cantatore, Paola Loguercio Polosa
      Pages 169-180

About this book

Proteins are the most diverse group of biologically important substances. With the recent technological advances in the genomics area and the efforts in proteomics research, the rate of discovery for new proteins with unknown structure and function has increased. These proteins generated from genomic approaches present enormous opportunities for research and industrial application. Protein Downstream Processing: Design, Development and Application of High and Low-Resolution Methods is a compilation of chapters within the exciting area of protein purification designed to give the laboratory worker the information needed to design and implement a successful purification strategy. It presents reliable and robust protocols in a concise form, emphasizing the critical aspects on practical problems and questions encountered at the lab bench. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Protein Downstream Processing: Design, Development and Application of High and Low-Resolution Methods will be an ideal source of scientific information to advanced students, junior researchers, and scientists involved in health sciences, cellular and molecular biology, biochemistry, and biotechnology and other related areas in both academia and industry.     ​

Editors and Affiliations

  • Laboratory of Enzyme Technology, Agricultural University of Athens Department of Agr. Biotechnology, Athens, Greece

    Nikolaos E. Labrou

Bibliographic Information

  • Book Title: Protein Downstream Processing

  • Book Subtitle: Design, Development and Application of High and Low-Resolution Methods

  • Editors: Nikolaos E. Labrou

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-977-2

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2014

  • Hardcover ISBN: 978-1-62703-976-5Published: 20 March 2014

  • Softcover ISBN: 978-1-4939-6040-8Published: 03 September 2016

  • eBook ISBN: 978-1-62703-977-2Published: 19 March 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 555

  • Number of Illustrations: 59 b/w illustrations, 62 illustrations in colour

  • Topics: Protein Science

Buy it now

Buying options

eBook USD 119.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access