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Arthritis Research

Volume 2: Methods and Protocols

Editors:

  • Broad range of topics covering many aspects of rheumatic disease
  • In-depth studies of cutting-edge technology
  • Robust tools for translational research
  • Contributions from leaders in their fields

Part of the book series: Methods in Molecular Medicine (MIMM, volume 136)

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Table of contents (27 protocols)

  1. Front Matter

    Pages i-xxii
  2. Immunobiology

    1. Front Matter

      Pages 1-1
    2. Phenotypic Analysis of B-Cells and Plasma Cells

      • Henrik E. Mei, Taketoshi Yoshida, Gwendolin Muehlinghaus, Falk Hiepe, Thomas Dörner, Andreas Radbruch et al.
      Pages 3-18
    3. Detection of Antigen Specific B-Cells in Tissues

      • Marie Wahren-Herlenius, Stina Salomonsson
      Pages 19-24
    4. Single Cell Analysis of Synovial Tissue B-Cells

      • Hye-Jung Kim, Claudia Berek
      Pages 25-37
    5. Tracking Antigen Specific CD4+ T-Cells With Soluble MHC Molecules

      • John A. Gebe, William W. Kwok
      Pages 39-50
    6. Analysis of Antigen Reactive T-Cells

      • Clare Alexander, Richard C. Duggleby, Jane C. Goodall, Malgosia K. Matyszak, Natasha Telyatnikova, J. S. Hill Gaston
      Pages 51-68
    7. Identification and Manipulation of Antigen Specific T-Cells with Artificial Antigen Presenting Cells

      • Eva Koffeman, Elissa Keogh, Mark Klein, Berent Prakken, Salvatore Albani
      Pages 69-86
    8. Analysis of Th1/Th2 T-Cell Subsets

      • Alla Skapenko, Hendrik Schulze-Koops
      Pages 87-96
    9. Analysis of the T-Cell Receptor Repertoire of Synovial T-Cells

      • Lucy R. Wedderburn, Douglas J. King
      Pages 97-116
    10. The Assessment of T-Cell Apoptosis in Synovial Fluid

      • Karim Raza, Dagmar Scheel-Toellner, Janet M. Lord, Arne N. Akbar, Christopher D. Buckley, Mike Salmon
      Pages 117-138
    11. Assays of T-Cell Contact Dependent Monocyte-Macrophage Functions

      • Danielle Burger, Jean-Michel Dayer
      Pages 139-148
    12. Phenotypic and Functional Analysis of Synovial Natural Killer Cells

      • Nicola Dalbeth, Margaret F. C. Callan
      Pages 149-163
    13. Identification and Isolation of Synovial Dendritic Cells

      • Allison R. Pettit, Lois Cavanagh, Amanda Boyce, Jagadish Padmanabha, Judy Peng, Ranjeny Thomas
      Pages 165-181
  3. Animal Models of Arthritis

    1. Front Matter

      Pages 183-183
    2. Collagen-Induced Arthritis in Mice

      • Richard O. Williams
      Pages 191-199
    3. Collagen-Induced Arthristis in Rats

      • Marie M. Griffiths, Grant W. Cannon, Tim Corsi, Van Reese, Kandie Kunzler
      Pages 201-214
    4. Collagen Antibody Induced Arthritis

      • Kutty Selva Nandakumar, Rikard Holmdahl
      Pages 215-223
    5. Arthritis Induced with Minor Cartilage Proteins

      • Stefan Carlsen, Shemin Lu, Rikard Holmdahl
      Pages 225-242

About this book

Arthritis Research: Methods and Protocols is a compendium of data pertinent to the methods and protocols that have contributed to recent advances in molecular medicine in general, but to the molecular basis of rheumatic disease in particular. These volumes details novel technologies, some of which are still evolving and whose impacts are yet to be determined. Leaders in the field contribute to cover such exciting and cutting edge topics as imaging and immunohistochemistry, analysis of cartilage and bone catabolism, immunobiology, and cell trafficking.

In Volume 1, authors discuss synovial joint morphology, histopathology, and immunohistochemistry, cartilage matrix and bone biology, and cell trafficking, migration and invasion. Volume 2 is broken up into sections that cover immunobiology and T cells, animal models of arthritis, and applications of new technologies, such as Differential Display Reverse Transcription-Polymerase Chain Reaction (DDRT-PCR), to define novel therapeutic targets. Both volumes combine to produce a concise set of protocols condensing decades of experience and expertise.

Arthritis Research: Methods and Protocols will be a valuable tool for basic research investigators, clinician scientists, pathologists, immunologists, and biochemists looking to stay current in their fields.

Reviews

From the reviews:

"This volume excels in the presentation of basic, fundamental techniques requisite in the anaylsis of arthritic disease.  Especially well done are the immunobiology and animal model sections." -Doody's Book Review, Weighted Numerical Score:96 - 4 Stars

"This multiauthored … compendium of arthritis research methods and protocols provides pertinent practical information for the bench researcher as well as for the interested clinician. … It is a worthwhile reference to have in the research laboratory … . I enthusiastically recommend … and believe this publication would have broad appeal to the rheumatologic community and to those in related specialties. … I congratulate the authors on developing an excellent compendium of arthritis research … ." (Robert J. Quinet, JAMA, April, 2008)

Editors and Affiliations

  • The Kennedy Institute of Rheumatology, Imperial College London, London, UK

    Andrew P. Cope

Bibliographic Information

  • Book Title: Arthritis Research

  • Book Subtitle: Volume 2: Methods and Protocols

  • Editors: Andrew P. Cope

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1007/978-1-59745-402-5

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2007

  • Hardcover ISBN: 978-1-58829-918-5Published: 06 July 2007

  • Softcover ISBN: 978-1-61737-845-4Published: 10 November 2010

  • eBook ISBN: 978-1-59745-402-5Published: 02 February 2008

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 1

  • Number of Pages: XXII, 432

  • Number of Illustrations: 73 b/w illustrations, 6 illustrations in colour

  • Topics: Orthopedics, Human Genetics, Cell Biology

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access