Embryonic Stem Cell Protocols

1. Front Matter

   Pages i-xxi

   PDF

2. Isolation and Maintenance

   1. Front Matter

      Pages 1-1

      PDF

   2. Isolation and Differentiation of Medaka Embryonic Stem Cells

      • Yunhan Hong, Manfred Schartl

      Pages 3-16

   3. Maintenance of Chicken Embryonic Stem Cells In Vitro

      • Hiroyuki Horiuchi, Shuichi Furusawa, Haruo Matsuda

      Pages 17-34

   4. Derivation and Culture of Mouse Trophoblast Stem Cells In Vitro

      • Satoshi Tanaka

      Pages 35-44

   5. Derivation, Maintenance, and Characterization of Rat Embryonic Stem Cells In Vitro

      • Maren Schulze, Hendrik Ungefroren, Michael Bader, Fred Fändrich

      Pages 45-58

   6. Derivation, Maintenance, and Induction of the Differentiation In Vitro of Equine Embryonic Stem Cells

      • Shigeo Saito, Ken Sawai, Arika Minamihashi, Hideyo Ugai, Takehide Murata, Kazunari K. Yokoyama

      Pages 59-79

   7. Generation and Characterization of Monkey Embryonic Stem Cells

      • Hirofumi Suemori, Norio Nakatsuji

      Pages 81-89

   8. Derivation and Propagation of Embryonic Stem Cells in Serum- and Feeder-Free Culture

      • Jennifer Nichols, Qi-Long Ying

      Pages 91-98

3. Signaling in Embryonic Stem Cell Differentiation

   1. Front Matter

      Pages 99-99

      PDF

   2. Internal Standards in Differentiating Embryonic Stem Cells In Vitro

      • Christopher L. Murphy

      Pages 101-112

   3. Matrix Assembly, Cell Polarization, and Cell Survival

      • Shaohua Li, Peter D. Yurchenco

      Pages 113-125

   4. Phosphoinositides, Inositol Phosphates, and Phospholipase C in Embryonic Stem Cells

      • Leo R. Quinlan

      Pages 127-149

   5. Cripto Signaling in Differentiating Embryonic Stem Cells

      • Gabriella Minchiotti, Silvia Parisi, M. Graziella Persico

      Pages 151-170

   6. The Use of Embryonic Stem Cells to Study Hedgehog Signaling

      • Sandy Becker, Laura Grabel

      Pages 171-185

   7. Transfection and Promoter Analysis in Embryonic Stem Cells

      • Sangmi Chung, Kwang-Soo Kim

      Pages 187-193

   8. SAGE Analysis to Identify Embryonic Stem Cell-Predominant Transcripts

      • Kenneth R. Boheler, Kirill V. Tarasov

      Pages 195-221

   9. Utilization of Digital Differential Display to Identify Novel Targets of Oct3/4

      • Yoshimi Tokuzawa, Masayoshi Maruyama, Shinya Yamanaka

      Pages 223-231

   10. Gene Silencing Using RNA Interference in Embryonic Stem Cells

       • J. Matthew Velkey, Nicole A. Slawny, Theresa E. Gratsch, K. Sue O’shea

       Pages 233-261

4. Genetic Manipulation of Embryonic Stem Cells

   1. Front Matter

      Pages 263-263

      PDF

Now in two volumes, this completely updated and expanded edition of Embryonic Stem Cells: Methods and Protocols provides a diverse collection of readily reproducible cellular and molecular protocols for the manipulation of nonhuman embryonic stem cells. Volume one, Embryonic Stem Cell Protocols: Isolation and Characterization, Second Edition, provides a diverse collection of readily reproducible cellular and molecular protocols for the isolation, maintenance, and characterization of embryonic stem cells. The second volume, Embryonic Stem Cell Protocols: Differentiation Models, Second Edition, covers state-of-the-art methods for deriving many types of differentiating cells from ES cells. Together, the two volumes illuminate for both novices and experts our current understanding of the biology of embryonic stem cells and their utility in normal tissue homeostasis and regenerative medicine applications.

• DNA
• Promoter
• Vivo
• gene expression
• gene transfer
• genes
• transgene expression

From Reviews of the First Edition...

"...elegantly introduces tremendous methods and protocols in ES studies...one of the most useful books that I have ever read in this field..." -Cell Biology International

"...highly valuable for any scientist who wants to make a start in the exciting field but also for experienced ES cell researchers who want to widen their repertoire" -Diabetologia

"...a very informative resource for any developmental or cell biologist with an interest in developments and prospects of ES cell research" -Molecular Biotechnology

"...a useful companion volume to other more specialized ES cell books..." -Nature Cell Biology

From the reviews of the second edition:

"Volume 1 describes methods for stem cell isolation and characterization. … written for basic science and clinical researchers interested in cellular and developmental biology, biochemistry, and molecular biology. Biomedical researchers interested in bioengineering, gene therapy, oncology, and regenerative medicine will also appreciate the methods described here. … The protocols are typically organized around specific research questions, and numerous examples of research findings are presented. … a welcome resource for research scientists interested in stem cell biology." (Bruce A Fenderson, Doody’s Review Service, July, 2006)

• Ottawa Health Research Institute, Ottawa, Canada

  Kursad Turksen

Policies and ethics