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Forensic DNA Typing Protocols

  • Book
  • © 2005

Overview

Editors:
  1. Angel Carracedo

    1. Institute of Legal Medicine, University of Santiago de Compostela, Galicia, Spain

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Part of the book series: Methods in Molecular Biology (MIMB, volume 297)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Forensic DNA-Typing Technologies

    • Angel Carracedo, Paula Sánchez-Diz
    Pages 1-11

  3. DNA Extraction and Quantitation of Forensic Samples Using the Phenol-Chloroform Method and Real-Time PCR

    • Silvano Köchl, Harald Niederstätter, Walther Parson
    Pages 13-29

  4. A Real-Time PCR Protocol to Determine the Number of Amelogenin (X-Y) Gene Copies From Forensic DNA Samples

    • Antonio Alonso, Pablo Martín
    Pages 31-44

  5. Species Determination

    • Adrian Linacre, James Chun-I Lee
    Pages 45-51

  6. Constructing STR Multiplex Assays

    • John M. Butler
    Pages 53-65

  7. Y Chromosome STR Typing

    • Leonor Gusmão, Cíntia Alves
    Pages 67-81

  8. Using Online Databases for Developing SNP Markers of Forensic Interest

    • Christopher Phillips
    Pages 83-105

  9. SNP Typing in Forensic Genetics

    • Beatriz Sobrino, Angel Carracedo
    Pages 107-126

  10. Genotyping SNPs With the LightCycler

    • Mará Victoria Lareu, Clara Ruiz-Ponte
    Pages 127-140

  11. Universal Tag Arrays in Forensic SNP Analysis

    • Marie Allen, Anna-Maria Divne
    Pages 141-153

  12. SNP Typing on the NanoChip Electronic Microarray

    • Claus BØrsting, Juan J. Sanchez, Niels Morling
    Pages 155-167

  13. Genotyping SNPs Using a UV-Photocleavable Oligonucleotide in MALDI-TOF MS

    • Peter M. Vallone, Kristina Fahr, Markus Kostrzewa
    Pages 169-178

  14. Mitochondrial D-Loop and Coding Sequence Analysis Using Pyrosequencing

    • Marie Allen, Hanna Andréasson
    Pages 179-195

  15. SNaPshot Typing of Mitochondrial DNA Coding Region Variants

    • Antonio Salas, Beatriz Quintáns, Vanesa Álvarez-Iglesias
    Pages 197-208

  16. Typing of Y Chromosome SNPs With Multiplex PCR Methods

    • Juan J. Sanchez, Claus Børsting, Niels Morling
    Pages 209-228

  17. Y Chromosome SNP Analysis Using the Single-Base Extension

    • María Brión
    Pages 229-242

  18. SNaPshot for Pharmacogenetics by Minisequencing

    • Klaus Bender
    Pages 243-252

  19. Methods for the Study of Ancient DNA

    • Christine Keyser-Tracqui, Bertrand Ludes
    Pages 253-264

  20. Protocols for Ancient DNA Typing

    • Cristian Capelli, Frank Tschentscher
    Pages 265-278
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Keywords

About this book

A state-of-the-art collection of readily reproducible laboratory methods for DNA identity analysis, including Y chromosome haplotyping, mtDNA, and SNP typing. The book offers well-tested protocols for DNA quantification using real-time PCR on forensic samples and for the determination of the number of amelogenine gene copies. For forensic geneticists, there are readily reproducible methods for species identification, ancient DNA, and pharmacogenetics. Additional chapters address new applications in the forensic genetics lab, such a species identification or typing of CYP polymorphisms for the analysis of adverse to drugs.

Editors and Affiliations

  • Institute of Legal Medicine, University of Santiago de Compostela, Galicia, Spain

    Angel Carracedo

Bibliographic Information

  • Book Title: Forensic DNA Typing Protocols

  • Editors: Angel Carracedo

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592598676

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2005

  • Hardcover ISBN: 978-1-58829-264-3Published: 30 November 2004

  • Softcover ISBN: 978-1-61737-462-3Published: 10 November 2010

  • eBook ISBN: 978-1-59259-867-0Published: 02 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 280

  • Number of Illustrations: 81 b/w illustrations

  • Topics: Forensic Medicine

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