Heme, Chlorophyll, and Bilins

1. Front Matter

   Pages i-ix

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2. Laboratory Methods for the Study of Tetrapyrroles

   • Alison G. Smith, Michael Witty

   Pages 1-12

3. Syntheses of Tetrapyrroles

   • Kevin M. Smith

   Pages 13-38

4. General Laboratory Methods for Tetrapyrroles

   • Jerry C. Bommer, Peter Hambright

   Pages 39-67

5. Enzymatic Preparation of Tetrapyrrole Intermediates

   • Martin J. Warren, Peter M. Shoolingin-Jordan

   Pages 69-93

6. Analysis of Biosynthetic Intermediates, 5-Aminolevulinic Acid to Heme

   • Chang Kee Lim

   Pages 95-109

7. Analysis of Intermediates and End Products of the Chlorophyll Biosynthetic Pathway

   • Constantin A. Rebeiz

   Pages 111-155

8. Analysis of Heme and Hemoproteins

   • Angela Wilks

   Pages 157-184

9. Hemoproteins Purification and Characterization by Using Aqueous Two-Phase Systems

   • Daniel Forciniti

   Pages 185-208

10. Structural Study of Heme Proteins by Electron Microscopy of 2-Dimensional Crystals

    • Terrence G. Frey

    Pages 209-234

11. Analysis and Reconstitution of Chlorophyll-Proteins

    • Harald Paulsen, Volkmar H. R. Schmid

    Pages 235-253

12. Two-Dimensional Crystallization of Chlorophyll Proteins

    • Georgios Tsiotis

    Pages 255-271

13. Biosynthesis and Analysis of Bilins

    • Matthew J. Terry

    Pages 273-291

14. Analysis and Reconstitution of Phytochromes

    • Michael T. McDowell, J. Clark Lagarias

    Pages 293-309

15. Analysis and Reconstitution of Phycobiliproteins: Methods for the Characterization of Bilin Attachment Reactions

    • Wendy M. Schluchter, Donald A. Bryant

    Pages 311-334

16. Back Matter

    Pages 335-340

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In Heme, Chlorophyll, and Bilins: Methods and Protocols, an interdisciplinary panel of hands-on investigators describe in detail how to work successfully with chlorophyll, heme, and bilins in biological, medical, chemical, and biochemical research. Each method is presented by a researcher who actually uses it on a daily basis and includes step-by-step instructions and pertinent tricks-of-the-trade that often make the difference between laboratory success and failure. Topics range from methods for the analysis of tetrapyrroles,heme, and hemoproteins, to the biosynthesis and analysis of chlorophyll and bilins.

• biological
• biosynthesis
• crystal
• crystallization
• electron
• electron microscopy
• intermediate
• laboratory
• microscopy
• phase
• protein
• proteins
• purification
• reactions
• synthesis

"This handy reference book features a collection of techniques written by experts in the field for working successfully with the title classes of compounds." - Journal of the American Chemical Society

• Department of Plant Sciences, University of Cambridge, Cambridge, UK

  Alison G. Smith

• Department of Biochemistry, University of Cambridge, Cambridge, UK

  Michael Witty

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