Overview
- Explains in detail a range of methods used to test altered behavioral phenotypes in various developmental stages of Drosophila
- Discusses protocols for the biochemical estimation of reactive oxygen species, metabolites, and stress enzymes
- Provides various histological fixation methods for detecting the structural arrangement in the flight muscle, eye, gut, and hemolymph of Drosophila under various microscopes
- Includes protocols for assessing the cytogenotoxicity, fat detection and metabolic activity in flies using fluorescent microscope and microplate reader
Part of the book series: Springer Protocols Handbooks (SPH)
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Table of contents (19 protocols)
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Front Matter
Keywords
About this book
This volume includes in-depth, hands-on protocols for detecting various developmental defects in Drosophila. It provides cutting-edge methods for maintaining Drosophila under laboratory conditions to perform various experiments, and for dissecting various imaginal discs of the larvae. Further, biochemical protocols for estimating the levels of different metabolites, reactive oxygen species, and fat-sensitive pathways are discussed, and various staining and behavior techniques for fat detection are provided.
The book explains how various fluorescent dyes and the comet assay can be used to identify DNA damage, and elaborates on the analysis of the eye, antennae, imaginal disc, gut and muscle damage under bright field and fluorescent microscopes. It covers the analysis of hemolymph using Giemsa staining; determining the functionality of channel proteins, eye and mechanosensory organs in adults and larvae using various behavioral assays; and metal detection and structural analysis for various tissues using SEM. In closing, it addresses the analysis of Drosophila’s eye and head via paraffin section; measurement of reactive oxygen species from various tissues via FACS; and the CRISPER technique for gene editing and analysis of micro RNA mutations.
Editors and Affiliations
About the editor
Dr. Monalisa Mishra is an Assistant Professor at the Department of Life Sciences, National Institute of Technology (NIT) in Rourkela, India. She completed her Ph.D. at the University of Bremen, Germany, prior to postdoctoral studies at the School of Medicine, University of San Diego, California, USA; Department of Biological Sciences, Indiana University, Bloomington, USA; and Max Plank Institute of Cell Biology and Genetics, Dresden, Germany.
Further, she previously served as an Assistant Professor at the Department of Biological Sciences, BITS in Pilani, India from 2012-2014. Currently, her lab at the NIT Rourkela is investigating various factors that can affect the development of Drosophila’s sensory organs. The lab’s findings have been published in many prominent international journals.
Bibliographic Information
Book Title: Fundamental Approaches to Screen Abnormalities in Drosophila
Editors: Monalisa Mishra
Series Title: Springer Protocols Handbooks
DOI: https://doi.org/10.1007/978-1-4939-9756-5
Publisher: Springer New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020
Hardcover ISBN: 978-1-4939-9755-8Published: 18 January 2020
Softcover ISBN: 978-1-4939-9758-9Published: 26 August 2021
eBook ISBN: 978-1-4939-9756-5Published: 16 December 2019
Series ISSN: 1949-2448
Series E-ISSN: 1949-2456
Edition Number: 1
Number of Pages: VII, 265
Number of Illustrations: 5 b/w illustrations, 111 illustrations in colour
Topics: Developmental Biology, Behavioral Sciences, Animal Models, Metabolomics, Biological Microscopy