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Antigen Processing

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Peter van Endert

    1. INSERM U1151, Université Paris Descartes, Hopital Necker-Enfants Malades, Paris, France

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1988)

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Table of contents (30 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Purification of Large Cytosolic Proteases for In Vitro Assays: 20S and 26S Proteasomes

    • Stefan Tenzer, Hansjörg Schild
    Pages 1-14

  3. Analysis of Proteasome-Generated Antigenic Peptides by Mass Spectrometry

    • Kathrin Textoris-Taube, Ulrike Kuckelkorn, Christin Beier, Peter M. Kloetzel
    Pages 15-29

  4. Trimming of MHC Class I Ligands by ERAP Aminopeptidases

    • Mirjana Weimershaus, Irini Evnouchidou, Lenong Li, Peter van Endert, Marlene Bouvier
    Pages 31-43

  5. Peptide Trimming for MHC Class I Presentation by Endoplasmic Reticulum Aminopeptidases

    • Mirco Compagnone, Doriana Fruci
    Pages 45-57

  6. Testing the Impact of Protease Inhibitors in Antigen Presentation Assays

    • Michael Basler, Marcus Groettrup
    Pages 59-69

  7. In Vitro Studies of MHC Class I Peptide Loading and Exchange

    • Marlene Bouvier
    Pages 71-81

  8. Measuring Synthesis and Degradation of MHC Class I Molecules

    • Simon J. Powis, Antony N. Antoniou
    Pages 83-100

  9. Biochemical Analysis of Naturally Processed Antigenic Peptides Presented by MHC Class I Molecules

    • Takayuki Kanaseki, Nilabh Shastri
    Pages 101-108

  10. A SIINFEKL-Based System to Measure MHC Class I Antigen Presentation Efficiency and Kinetics

    • Devin Dersh, Jonathan W. Yewdell, Jiajie Wei
    Pages 109-122

  11. Purification and Identification of Naturally Presented MHC Class I and II Ligands

    • Annika Nelde, Daniel J. Kowalewski, Stefan Stevanović
    Pages 123-136

  12. Identification of MHC Ligands and Establishing MHC Class I Peptide Motifs

    • Michael Ghosh, Moreno Di Marco, Stefan Stevanović
    Pages 137-147

  13. Quantitating MHC Class I Ligand Production and Presentation Using TCR-Like Antibodies

    • Brian P. Dolan
    Pages 149-157

  14. Identifying Antigens Recognized by Cytolytic T Lymphocytes on Tumors

    • Nathalie Vigneron, Vincent Stroobant, Wenbin Ma, Alexandre Michaux, Benoît J. Van den Eynde
    Pages 159-186

  15. A Flow Cytometry-Based Approach to Unravel Viral Interference with the MHC Class I Antigen Processing and Presentation Pathway

    • Patrique Praest, Hendrik de Buhr, Emmanuel J. H. J. Wiertz
    Pages 187-198

  16. Evaluating CD8+ T Cell Responses In Vitro

    • François A. Lemonnier
    Pages 199-215

  17. Recombinant Poxviruses: Versatile Tools for Immunological Assays

    • Katherine S. Forsyth, Mary M. Addison, Laurence C. Eisenlohr
    Pages 217-248

  18. Monitoring Intracellular Routing of Internalized Antigens by Immunofluorescence Microscopy

    • Maria Embgenbroich, Jasmin Mertins, Thorsten Lang, Sven Burgdorf
    Pages 249-257

  19. Monitoring MHC Ubiquitination by MARCH Ubiquitin Ligases

    • Evelina Gatti
    Pages 259-270

  20. Monitoring MHC-II Endocytosis and Recycling Using Cell-Surface Protein Biotinylation-Based Assays

    • Kyung-Jin Cho, Paul A. Roche
    Pages 271-277
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Keywords

About this book

The second edition volume expands on the previous edition with new and updated chapters on the latest methods used for studying presentation of antigenic peptides produced in the standard processing pathways for MHC class I and II molecules. The new chapters cover topics such as biochemical and cellular approaches to study the impact of the endoplasmic reticulum aminopeptidases; techniques to monitor MHC class I synthesis and degradation; approaches to measure processing efficacy; description of different assays measuring MHC recycling; and protocols to produce MHC class II tetramers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Cutting-edge and comprehensive, Antigen Processing: Methods and Protocols, Second Edition is a valuable tool for both novice and expert researchers interested in studying antigen processing and venturing out further into this evolving field.


Editors and Affiliations

  • INSERM U1151, Université Paris Descartes, Hopital Necker-Enfants Malades, Paris, France

    Peter van Endert

Bibliographic Information

  • Book Title: Antigen Processing

  • Book Subtitle: Methods and Protocols

  • Editors: Peter van Endert

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9450-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9449-6Published: 30 May 2019

  • Softcover ISBN: 978-1-4939-9452-6Published: 14 August 2020

  • eBook ISBN: 978-1-4939-9450-2Published: 30 May 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XV, 458

  • Number of Illustrations: 34 b/w illustrations, 45 illustrations in colour

  • Topics: Molecular Medicine, Antibodies

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