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  • © 2018

Glioblastoma

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1741)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
  2. Whole Genome Sequencing-Based Discovery of Structural Variants in Glioblastoma

    • Kazimierz O. Wrzeszczynski, Vanessa Felice, Minita Shah, Sadia Rahman, Anne-Katrin Emde, Vaidehi Jobanputra et al.
    Pages 1-29
  3. Establishing Primary Human Glioblastoma Adherent Cultures from Operative Specimens

    • Amit D. Gujar, Diane D. Mao, John B. Finlay, Albert H. Kim
    Pages 53-62
  4. Establishing Primary Human Glioblastoma Tumorsphere Cultures from Operative Specimens

    • Joshua D. Frenster, Dimitris G. Placantonakis
    Pages 63-69
  5. Isolation of Glioblastoma Stem Cells with Flow Cytometry

    • Julio Inocencio, Joshua D. Frenster, Dimitris G. Placantonakis
    Pages 71-79
  6. Lentiviral Transduction of Primary Human Glioblastoma Cultures

    • Joshua D. Frenster, Julio Inocencio, Dimitris G. Placantonakis
    Pages 81-89
  7. Intracellular pH Measurements in Glioblastoma Cells Using the pH-Sensitive Dye BCECF

    • Nataliya V. Galifianakis, Dimitris G. Placantonakis, Mitchell Chesler
    Pages 103-109
  8. Induction and Assessment of Hypoxia in Glioblastoma Cells In Vitro

    • Jean-Pierre Gagner, Mirna Lechpammer, David Zagzag
    Pages 111-123
  9. Sample Preparation for Relative Quantitation of Proteins Using Tandem Mass Tags (TMT) and Mass Spectrometry (MS)

    • Hediye Erdjument-Bromage, Fang-Ke Huang, Thomas A. Neubert
    Pages 135-149
  10. Single-Cell RNA Sequencing of Glioblastoma Cells

    • Rajeev Sen, Igor Dolgalev, N. Sumru Bayin, Adriana Heguy, Aris Tsirigos, Dimitris G. Placantonakis
    Pages 151-170
  11. Evaluation of Radioresponse and Radiosensitizers in Glioblastoma Organotypic Cultures

    • N. Sumru Bayin, Lin Ma, Dimitris G. Placantonakis, Mary Helen Barcellos-Hoff
    Pages 171-182
  12. Orthotopic Patient-Derived Glioblastoma Xenografts in Mice

    • Zhongye Xu, Michael Kader, Rajeev Sen, Dimitris G. Placantonakis
    Pages 183-190
  13. Bioluminescent In Vivo Imaging of Orthotopic Glioblastoma Xenografts in Mice

    • Joshua D. Frenster, Dimitris G. Placantonakis
    Pages 191-198
  14. Flow Cytometric Identification of Tumor-Infiltrating Lymphocytes from Glioblastoma

    • Karolina Woroniecka, Pakawat Chongsathidkiet, Aladine Elsamadicy, Harrison Farber, Xiuyu Cui, Peter E. Fecci
    Pages 221-226
  15. Modeling Glioma with Human Embryonic Stem Cell-Derived Neural Lineages

    • Aram S. Modrek, Jod Prado, Devin Bready, Joravar Dhaliwal, Danielle Golub, Dimitris G. Placantonakis
    Pages 227-237

About this book

This volume details cutting-edge protocols on the characterization of the genome, epigenome, proteome, metabolome and single-cell transcriptome of tumors and tumor-derived cultures. Chapters focus on subpopulations of cells with stem-like properties, laser capture microdissection, and modeling human glioma with human embryonic stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Glioblastoma: Methods and Protocols aims to support researchers seeking new and refined protocols to better decrypt this tumor. 

Editors and Affiliations

  • Department of Neurosurgery, NYU School of Medicine, New York, USA

    Dimitris G. Placantonakis

Bibliographic Information

  • Book Title: Glioblastoma

  • Book Subtitle: Methods and Protocols

  • Editors: Dimitris G. Placantonakis

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7659-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2018

  • Hardcover ISBN: 978-1-4939-7658-4Published: 02 February 2018

  • Softcover ISBN: 978-1-4939-8536-4Published: 04 June 2019

  • eBook ISBN: 978-1-4939-7659-1Published: 31 January 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 241

  • Number of Illustrations: 9 b/w illustrations, 43 illustrations in colour

  • Topics: Cancer Research

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 119.00
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access