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B Cell Receptor Signaling

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Chaohong Liu

    1. Department of Pathogen Biology, Huazhong University of Science and Technology, Huazhong, China

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1707)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Activation-Induced Cytidine Deaminase Aided In Vitro Antibody Evolution

    • Lili An, Chuan Chen, Ruiqi Luo, Yun Zhao, Haiying Hang
    Pages 1-14

  3. Analyzing Mouse B Cell Responses Specific to LCMV Infection

    • Yaxing Hao, Zhirong Li, Yifei Wang, Xiaobing Liu, Lilin Ye
    Pages 15-38

  4. Expression of Exogenous Genes in Murine Primary B Cells and B Cell Lines Using Retroviral Vectors

    • Zhiyong Yang, Christopher D. C. Allen
    Pages 39-49

  5. Biophysical Techniques to Study B Cell Activation: Single-Molecule Imaging and Force Measurements

    • Ivan Rey, David A. Garcia, Brittany A. Wheatley, Wenxia Song, Arpita Upadhyaya
    Pages 51-68

  6. DNA-Based Probes for Measuring Mechanical Forces in Cell-Cell Contacts: Application to B Cell Antigen Extraction from Immune Synapses

    • Katelyn M. Spillane, Pavel Tolar
    Pages 69-80

  7. Deriving Quantitative Cell Biological Information from Dye-Dilution Lymphocyte Proliferation Experiments

    • Koushik Roy, Maxim Nikolaievich Shokhirev, Simon Mitchell, Alexander Hoffmann
    Pages 81-94

  8. Flow Cytometry Analysis of mTOR Signaling in Antigen-Specific B Cells

    • Qizhao Huang, Haoqiang Wang, Lifan Xu, Jianjun Hu, Pengcheng Wang, Yiding Li et al.
    Pages 95-109

  9. Ex Vivo Culture Assay to Measure Human Follicular Helper T (Tfh) Cell-Mediated Human B Cell Proliferation and Differentiation

    • Xin Gao, Lin Lin, Di Yu
    Pages 111-119

  10. B Cell Receptor Signaling and Compartmentalization by Confocal Microscopy

    • Anurag R. Mishra, Akanksha Chaturvedi
    Pages 121-129

  11. Imaging the Interactions Between B Cells and Antigen-Presenting Cells

    • Jia C. Wang, Madison Bolger-Munro, Michael R. Gold
    Pages 131-161

  12. In Vivo Tracking of Particulate Antigen Localization and Recognition by B Lymphocytes at Lymph Nodes

    • Yolanda R. Carrasco
    Pages 163-169

  13. Study B Cell Antigen Receptor Nano-Scale Organization by In Situ Fab Proximity Ligation Assay

    • Kathrin Kläsener, Jianying Yang, Michael Reth
    Pages 171-181

  14. Single-Particle Tracking of Cell Surface Proteins

    • Laabiah Wasim, Bebhinn Treanor
    Pages 183-192

  15. The Use of Intravital Two-Photon and Thick Section Confocal Imaging to Analyze B Lymphocyte Trafficking in Lymph Nodes and Spleen

    • Chung Park, Il-Young Hwang, John H. Kehrl
    Pages 193-205

  16. Time-Lapse Förster Resonance Energy Transfer Imaging by Confocal Laser Scanning Microscopy for Analyzing Dynamic Molecular Interactions in the Plasma Membrane of B Cells

    • Hae Won Sohn, Joseph Brzostowski
    Pages 207-224

  17. Understanding of B Cell Receptor Signaling Through a Photo-Activatable Antigen Presentation System

    • Jing Wang, Zhengpeng Wan, Wanli Liu
    Pages 225-234

  18. Use of Streptolysin O-Induced Membrane Damage as a Method of Studying the Function of Lipid Rafts During B Cell Activation

    • Heather Miller, Wenxia Song
    Pages 235-241

  19. Visualization and Quantitative Analysis of the Actin Cytoskeleton Upon B Cell Activation

    • Vid Å uÅ¡tar, Marika Vainio, Pieta K. Mattila
    Pages 243-257

  20. Back Matter

    Pages 259-262
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Keywords

About this book

This volume looks the current advanced protocols used to study aspects of the B cell receptor (BCR). The chapters in this book cover topics such as the mutant of BCR repertoire to understand antibody evolution; interactions between B cells and viruses; mechanical force during BCR activation; B cell signaling using flow cytometry; confocal microscopy, total internal reflection microscopy and intravital two-photon microscopy; and the methods used to study critical cell components related with B cell activation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, B Cell Receptor Signaling: Methods and Protocols is a valuable resource for everyone in the scientific community currently working inthe B cell field.

Editors and Affiliations

  • Department of Pathogen Biology, Huazhong University of Science and Technology, Huazhong, China

    Chaohong Liu

Bibliographic Information

  • Book Title: B Cell Receptor Signaling

  • Book Subtitle: Methods and Protocols

  • Editors: Chaohong Liu

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7474-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2018

  • Hardcover ISBN: 978-1-4939-7473-3Published: 01 February 2018

  • Softcover ISBN: 978-1-4939-8497-8Published: 04 June 2019

  • eBook ISBN: 978-1-4939-7474-0Published: 31 January 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 262

  • Number of Illustrations: 19 b/w illustrations, 48 illustrations in colour

  • Topics: Biochemistry, general

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