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Plant Membrane Proteomics

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Hans-Peter Mock

    1. Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany

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  2. Andrea Matros

    1. Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany

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    You can also search for this editor in PubMed   Google Scholar

  3. Katja Witzel

    1. Leibniz Institute of Vegetable and Ornamental Crops, Großbeeren, Germany

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1696)

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About this book

This volume provides comprehensive and detailed  protocols that discuss proteomic techniques, plant endosomes, and isolation of organelles and subcellular fractions. The chapters in this book explore numerous plant species and cover topics, such as isolation and purity assessment of membranes from Norway spruce; proteomic analysis of nuclei; analyzing the vacuolar membrane (tonoplast) proteome; isoforms of a thylakoid-bound protein; assay of plasma membrane H+-ATPase in plant tissue under abiotic stresses; and identification and characterization of plant membrane proteins using ARAMEMNON. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Practical and thorough, Plant Membrane Proteomics: Methodsand Protocols is a valuable resource that promotes the use of plant membrane proteomics to develop the future of the field.


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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xii
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  2. Free Flow Zonal Electrophoresis for Fractionation of Plant Membrane Compartments Prior to Proteomic Analysis

    • Bronwyn J. Barkla
    Pages 1-12

  3. Isolation and Purity Assessment of Membranes from Norway Spruce

    • Enni Väisänen, Junko Takahashi, Luis A. Jiménez Barboza, Xianbao Deng, Teemu H. Teeri, Kurt V. Fagerstedt et al.
    Pages 13-39

  4. Nuclear Proteome: Isolation of Intact Nuclei, Extraction of Nuclear Proteins, and 2-DE Analysis

    • Aarti Pandey, Subhra Chakraborty, Niranjan Chakraborty
    Pages 41-55

  5. Identification of Plant Nuclear Proteins Based on a Combination of Flow Sorting, SDS-PAGE, and LC-MS/MS Analysis

    • Ivo Chamrád, Jana Uřinovská, Beáta Petrovská, Hana Jeřábková, René Lenobel, Jan Vrána et al.
    Pages 57-79

  6. Isolation, Purity Assessment, and Proteomic Analysis of Nuclei

    • Setsuko Komatsu
    Pages 81-90

  7. Proteomic Analysis of Rice Golgi Membranes Isolated by Floating Through Discontinuous Sucrose Density Gradient

    • Kazusato Oikawa, Takuya Inomata, Yoshitoshi Hirao, Tadashi Yamamoto, Marouane Baslam, Kentaro Kaneko et al.
    Pages 91-105

  8. Analyzing the Vacuolar Membrane (Tonoplast) Proteome

    • Miwa Ohnishi, Katsuhisa Yoshida, Tetsuro Mimura
    Pages 107-116

  9. Preparation of Membrane Fractions (Envelope, Thylakoids, Grana, and Stroma Lamellae) from Arabidopsis Chloroplasts for Quantitative Proteomic Investigations and Other Studies

    • Lucas Moyet, Daniel Salvi, Martino Tomizioli, Daphné Seigneurin-Berny, Norbert Rolland
    Pages 117-136

  10. Isolation of Intact Thylakoid Membranes from Heterocysts of Filamentous, Nitrogen-Fixing Cyanobacteria

    • Ann Magnuson, Tanai Cardona
    Pages 137-145

  11. Targeted Quantification of Isoforms of a Thylakoid-Bound Protein: MRM Method Development

    • Roque Bru-Martínez, Ascensión Martínez-Márquez, Jaime Morante-Carriel, Susana Sellés-Marchart, María José Martínez-Esteso, José Luis Pineda-Lucas et al.
    Pages 147-162

  12. Sample Preparation for Analysis of the Plant Mitochondrial Membrane Proteome

    • Christine Schikowsky, Beate Thal, Hans-Peter Braun, Holger Eubel
    Pages 163-183

  13. Plasma Membrane Proteomics of Arabidopsis Suspension-Cultured Cells Associated with Growth Phase Using Nano-LC-MS/MS

    • Bin Li, Daisuke Takahashi, Yukio Kawamura, Matsuo Uemura
    Pages 185-194

  14. Mini-Scale Isolation and Preparation of Plasma Membrane Proteins from Potato Roots for LC/MS Analysis

    • Anna M. Jozefowicz, Andrea Matros, Katja Witzel, Hans-Peter Mock
    Pages 195-204

  15. Assay of Plasma Membrane H+-ATPase in Plant Tissues under Abiotic Stresses

    • Małgorzata Janicka, Anna Wdowikowska, Grażyna Kłobus
    Pages 205-215

  16. Absolute Quantitation of In Vitro Expressed Plant Membrane Proteins by Targeted Proteomics (MRM) for the Determination of Kinetic Parameters

    • Carsten Rautengarten, Berit Ebert, Joshua L. Heazlewood
    Pages 217-234

  17. MSE for Label-Free Absolute Protein Quantification in Complex Proteomes

    • Stefan Helm, Sacha Baginsky
    Pages 235-247

  18. Identification and Characterization of Plant Membrane Proteins Using ARAMEMNON

    • Rainer Schwacke, Ulf-Ingo Flügge
    Pages 249-259

  19. VANTED: A Tool for Integrative Visualization and Analysis of -Omics Data

    • Anja Hartmann, Anna Maria Jozefowicz
    Pages 261-278

  20. Back Matter

    Pages 279-280
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Editors and Affiliations

  • Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany

    Hans-Peter Mock, Andrea Matros

  • Leibniz Institute of Vegetable and Ornamental Crops, Großbeeren, Germany

    Katja Witzel

Bibliographic Information

  • Book Title: Plant Membrane Proteomics

  • Book Subtitle: Methods and Protocols

  • Editors: Hans-Peter Mock, Andrea Matros, Katja Witzel

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7411-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2018

  • Hardcover ISBN: 978-1-4939-7409-2Published: 30 October 2017

  • Softcover ISBN: 978-1-4939-8477-0Published: 23 August 2018

  • eBook ISBN: 978-1-4939-7411-5Published: 27 October 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 280

  • Number of Illustrations: 14 b/w illustrations, 33 illustrations in colour

  • Topics: Plant Sciences

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