Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1685)
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Table of contents (21 protocols)
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Front Matter
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Computational Protocols
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Front Matter
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Protein Library Expression
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Front Matter
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Screening and Selection Assays
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Front Matter
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About this book
This volume details basic and advanced protocols for both stages of protein engineering: the library design phase and the identification of improved variants by screening and selection. Chapters focus on enzyme engineering using rational and semi-rational approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Protein Engineering: Methods and Protocols aims to aid scientists in the planning and performance of their experiments.
The chapter 'Functional Analysis of Membrane Proteins Produced by Cell-Free Translation' is open access under a CC BY 4.0 license via link.springer.com.
Editors and Affiliations
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Department of Biotechnology and Enzyme Catalysis Institute of Biochemistry, Greifswald University, Greifswald, Germany
Uwe T. Bornscheuer
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Protein Biochemistry Institute of Biochemistry, Greifswald University, Greifswald, Germany
Matthias Höhne
Bibliographic Information
Book Title: Protein Engineering
Book Subtitle: Methods and Protocols
Editors: Uwe T. Bornscheuer, Matthias Höhne
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-7366-8
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media LLC 2018
Hardcover ISBN: 978-1-4939-7364-4Published: 30 October 2017
Softcover ISBN: 978-1-4939-8463-3Published: 23 May 2018
eBook ISBN: 978-1-4939-7366-8Published: 27 October 2017
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 350
Number of Illustrations: 19 b/w illustrations, 63 illustrations in colour
Topics: Protein Science