Potassium Channels

1. Front Matter

   Pages i-xvi

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2. Manipulating Potassium Channel Expression and Function in Hippocampal Neurons by In Utero Electroporation

   • Kang Wang

   Pages 1-5

3. Studying KCNQ1 Mutation and Drug Response in Type 1 Long QT Syndrome Using Patient-Specific Induced Pluripotent Stem Cell-Derived Cardiomyocytes

   • Heming Wei, Jianjun Wu, Zhenfeng Liu

   Pages 7-28

4. Monitoring Changes in the Abundance of Endogenously Expressed ATP-Sensitive Potassium (KATP) Channels in the Plasma Membrane Using Surface Biotinylation

   • Jing-Syuna Ruan, Pei-Chun Chen

   Pages 29-36

5. Nonsense-Mediated mRNA Decay of hERG Mutations in Long QT Syndrome

   • Qiuming Gong, Zhengfeng Zhou

   Pages 37-49

6. Probing Subunits Interactions in K_ATP Channels Using Photo-Crosslinking via Genetically Encoded p-Azido-l-phenylalanine

   • Prasanna Devaraneni, Emily A. Rex, Show-Ling Shyng

   Pages 51-61

7. Hyper-SUMOylation of K+ Channels in Sudden Unexplained Death in Epilepsy: Isolation and Primary Culture of Dissociated Hippocampal Neurons from Newborn Mice for Subcellular Localization

   • Xu Chen, Shanshan Zhang, Jifang Huang, Wanying Dong, Hui Xiao, Huanjie Shao et al.

   Pages 63-71

8. Simultaneous Real-Time Measurement of the β-Cell Membrane Potential and Ca²⁺ Influx to Assess the Role of Potassium Channels on β-Cell Function

   • Nicholas C. Vierra, Matthew T. Dickerson, Louis H. Philipson, David A. Jacobson

   Pages 73-84

9. Methods for Characterizing Disease-Associated ATP-Sensitive Potassium Channel Mutations

   • Balamurugan Kandasamy, Show-Ling Shyng

   Pages 85-104

10. Thallium Flux Assay for Measuring the Activity of Monovalent Cation Channels and Transporters

    • C. David Weaver

    Pages 105-114

11. Nuclear Magnetic Resonance Approaches for Characterizing Protein-Protein Interactions

    • Yuki Toyama, Yoko Mase, Hanaho Kano, Mariko Yokogawa, Masanori Osawa, Ichio Shimada

    Pages 115-128

12. Studying Mechanosensitivity of Two-Pore Domain K+ Channels in Cellular and Reconstituted Proteoliposome Membranes

    • Josefina del Mármol, Robert A. Rietmeijer, Stephen G. Brohawn

    Pages 129-150

13. Migration of PIP2 on KCNQ2 Surface Revealed by Molecular Dynamics Simulations

    • Qiansen Zhang, Huaiyu Yang

    Pages 151-161

14. Studying Structural Dynamics of Potassium Channels by Single-Molecule FRET

    • Shizhen Wang, Joshua B. Brettmann, Colin G. Nichols

    Pages 163-180

15. Patch-Clamp Recordings of the KcsA K+ Channel in Unilamellar Blisters

    • Kimberly Matulef, Francis I. Valiyaveetil

    Pages 181-191

16. Combinatorial Assembly of Lumitoxins

    • David Nedrud, Daniel Schmidt

    Pages 193-209

17. Characterization of MC4R Regulation of the Kir7.1 Channel Using the Tl+ Flux Assay

    • Michael J. Litt, Roger D. Cone, Masoud Ghamari-Langroudi

    Pages 211-222

18. Stopped-Flow Fluorometric Ion Flux Assay for Ligand-Gated Ion Channel Studies

    • David J. Posson, Radda Rusinova, Olaf S. Andersen, Crina M. Nimigean

    Pages 223-235

19. In Vivo Analysis of Potassium Channelopathies: Loose Patch Recording of Purkinje Cell Firing in Living, Awake Zebrafish

    • Jui-Yi Hsieh, Diane M. Papazian

    Pages 237-252

20. Site-Directed Unnatural Amino Acid Mutagenesis to Investigate Potassium Channel Pharmacology in Xenopus laevis Oocytes

    • Robin Y. Kim, Harley T. Kurata

    Pages 253-263

This volume provides readers with the latest techniques and approaches used to study K+ channels. There are a wide range of topics covered in the book, such as channel structure; K+ channels and their roles in physiology and disease; single-molecule FRET; proteins expression levels in vivo; real-time correlation of channel activity; gating; pharmacology; physiological functions; and channelopathies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Cutting-edge and comprehensive, Potassium Channels: Methods and Protocols is a valuable resource for all researchers who are interested in studying potassium channels.

• membrane excitability
• ion channels
• channel structure
• channelopathies
• Patching K+ channels
• pharmacology

• Department of Physiology and Pharmacology, Oregon Health and Science University, Portland, USA

  Show-Ling Shyng, Francis I. Valiyaveetil

• The Vollum Institute, Oregon Health and Science University, Portland, USA

  Matt Whorton

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