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High Content Screening

A Powerful Approach to Systems Cell Biology and Phenotypic Drug Discovery

  • Book
  • © 2018

Overview

Editors:
  1. Paul A. Johnston

    1. Department of Pharmaceutical Sciences, School of Pharmacy, University of Pittsburgh, Pittsburgh, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Oscar J. Trask

    1. Bahama Bio, LLC, Bahama, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1683)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Reagents and Kits for Measuring Cells and their Phenotypes

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Applications and Caveats on the Utilization of DNA-Specific Probes in Cell-Based Assays

      • Roy Edward
      Pages 3-19

    3. General Staining and Segmentation Procedures for High Content Imaging and Analysis

      • Kevin M. Chambers, Bhaskar S. Mandavilli, Nick J. Dolman, Michael S. Janes
      Pages 21-31

    4. Tools to Measure Cell Health and Cytotoxicity Using High Content Imaging and Analysis

      • Bhaskar S. Mandavilli, Robert J. Aggeler, Kevin M. Chambers
      Pages 33-46

    5. Cell-Based High Content Analysis of Cell Proliferation and Apoptosis

      • Bhaskar S. Mandavilli, Michelle Yan, Scott Clarke
      Pages 47-57

    6. Tools to Measure Autophagy Using High Content Imaging and Analysis

      • Nick J. Dolman, Brent A. Samson, Kevin M. Chambers, Michael S. Janes, Bhaskar S. Mandavilli
      Pages 59-71

  3. Selecting Microtiter Plates, Identify Imaging Artifacts, RNAi Specificity, Data Handling, Kinetic Assays, and Ultra-High Throughput HCS

    1. Front Matter

      Pages 73-73
      Download chapter PDF

    2. Guidelines for Microplate Selection in High Content Imaging

      • Oscar J. Trask
      Pages 75-88

    3. Quality Control for High-Throughput Imaging Experiments Using Machine Learning in Cellprofiler

      • Mark-Anthony Bray, Anne E. Carpenter
      Pages 89-112

    4. High-Content Screening Approaches That Minimize Confounding Factors in RNAi, CRISPR, and Small Molecule Screening

      • Steven A. Haney
      Pages 113-130

    5. Strategies and Solutions to Maintain and Retain Data from High Content Imaging, Analysis, and Screening Assays

      • K. Kozak, B. Rinn, O. Leven, M. Emmenlauer
      Pages 131-148

    6. Live-Cell High Content Screening in Drug Development

      • Milan Esner, Felix Meyenhofer, Marc Bickle
      Pages 149-164

    7. Challenges and Opportunities in Enabling High-Throughput, Miniaturized High Content Screening

      • Debra Nickischer, Lisa Elkin, Normand Cloutier, Jonathan O’Connell, Martyn Banks, Andrea Weston
      Pages 165-191

  4. Harnessing Translocation, Signaling Pathways, Single Cell Analysis, and Multidimensional Data for Phenotypic Analysis

    1. Front Matter

      Pages 193-193
      Download chapter PDF

    2. Translocation Biosensors—Versatile Tools to Probe Protein Functions in Living Cells

      • Verena Fetz, Roland H. Stauber, Shirley K. Knauer
      Pages 195-210

    3. High Content Positional Biosensor Assay to Screen for Compounds that Prevent or Disrupt Androgen Receptor and Transcription Intermediary Factor 2 Protein-Protein Interactions

      • Yun Hua, Daniel P. Camarco, Christopher J. Strock, Paul A. Johnston
      Pages 211-227

    4. High Content Imaging Assays for IL-6-Induced STAT3 Pathway Activation in Head and Neck Cancer Cell Lines

      • Paul A. Johnston, Malabika Sen, Yun Hua, Daniel P. Camarco, Tong Ying Shun, John S. Lazo et al.
      Pages 229-244

    5. Single Cell and Population Level Analysis of HCA Data

      • David Novo, Kaya Ghosh, Sean Burke
      Pages 245-266

    6. Utilization of Multidimensional Data in the Analysis of Ultra-High-Throughput High Content Phenotypic Screens

      • Judith Wardwell-Swanson, Yanhua Hu
      Pages 267-290
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Keywords

About this book

This second edition details some of the recent trends in HCS/HCA/HCI.  New and updated chapters guide readers through methods that utilize reagents and kits that have been developed to measure cells and subpopulation classifications, control measures, overviews of the data handling issues associated with HCS/HCA/HCI, and methods to implement more complex phenotypic models.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, High Content Screening and Analysis-The Ideal Format for Phenotypic Screening: A Powerful Approach to Systems Cell Biology and Drug Discovery, Second Edition aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Department of Pharmaceutical Sciences, School of Pharmacy, University of Pittsburgh, Pittsburgh, USA

    Paul A. Johnston

  • Bahama Bio, LLC, Bahama, USA

    Oscar J. Trask

Bibliographic Information

  • Book Title: High Content Screening

  • Book Subtitle: A Powerful Approach to Systems Cell Biology and Phenotypic Drug Discovery

  • Editors: Paul A. Johnston, Oscar J. Trask

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7357-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2018

  • Hardcover ISBN: 978-1-4939-7355-2Published: 30 October 2017

  • Softcover ISBN: 978-1-4939-8461-9Published: 30 August 2018

  • eBook ISBN: 978-1-4939-7357-6Published: 27 October 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIII, 397

  • Number of Illustrations: 11 b/w illustrations, 71 illustrations in colour

  • Topics: Biotechnology

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