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Plant Respiration and Internal Oxygen

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1670)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xiii
  2. Assessing Metabolic Flux in Plants with Radiorespirometry

    • Nicholas J. Kruger, Shyam K. Masakapalli, R. George Ratcliffe
    Pages 1-16
  3. Coupling Radiotracer Experiments with Chemical Fractionation for the Estimation of Respiratory Fluxes

    • Toshihiro Obata, Laise Rosado-Souza, Alisdair R. Fernie
    Pages 17-30
  4. VisiSens Technique to Measure Internal Oxygen and Respiration in Barley Roots

    • Aprajita Kumari, Kapuganti Jagadis Gupta
    Pages 39-45
  5. MultiSense: A Multimodal Sensor Tool Enabling the High-Throughput Analysis of Respiration

    • Peter Keil, Gregor Liebsch, Ljudmilla Borisjuk, Hardy Rolletschek
    Pages 47-56
  6. Measurement of Respiration and Internal Oxygen in Germinating Cicer arietinum L. Seeds Using Optic Microsensor

    • Sonika Pandey, Aprajita Kumari, Chellapilla Bharadwaj, Kapuganti Jagadis Gupta
    Pages 57-62
  7. Isolation of Physiologically Active and Intact Mitochondria from Chickpea

    • Sonika Pandey, Aprajita Kumari, Kapuganti Jagadis Gupta
    Pages 77-85
  8. Isolation and Structural Studies of Mitochondria from Pea Roots

    • Abhaypratap Vishwakarma, Kapuganti Jagadis Gupta
    Pages 87-95
  9. Mitochondrial Respiration and Oxygen Tension

    • Daniel S. Shaw, Karlia Meitha, Michael J. Considine, Christine H. Foyer
    Pages 97-113
  10. Isolation of Mitochondria from Model and Crop Plants

    • Sandra M. Kerbler, Nicolas L. Taylor
    Pages 115-142
  11. Procedures of Mitochondria Purification and Gene Expression to Study Alternative Respiratory and Uncoupling Pathways in Fruits

    • Jurandi Gonçalves de Oliveira, Luis Miguel Mazorra Morales, Gláucia Michelle Cosme Silva, Kátia Daniella da Cruz Saraiva, Diederson Bortolino Santana, Clesivan Pereira dos Santos et al.
    Pages 143-165
  12. Measurement of Tricarboxylic Acid Cycle Enzyme Activities in Plants

    • Rebeca Patricia Omena-Garcia, Wagner L. Araújo, Yves Gibon, Alisdair R. Fernie, Adriano Nunes-Nesi
    Pages 167-182
  13. Measurements of Electron Partitioning Between Cytochrome and Alternative Oxidase Pathways in Plant Tissues

    • Nestor Fernandez Del-Saz, Miquel Ribas-Carbo, Gabriel Martorell, Alisdair R. Fernie, Igor Florez-Sarasa
    Pages 203-217
  14. A Step-by-Step Protocol for Classifying AOX Proteins in Flowering Plants

    • José Hélio Costa, Clesivan Pereira dos Santos, Kátia Daniella da Cruz Saraiva, Birgit Arnholdt-Schmitt
    Pages 225-234

About this book

This volume covers a wide range of methods to measure cellular respiration and internal oxygen in various tissues under different conditions. Chapters guide readers through informative experimental approaches, calorespirometry, isotope fractionation techniques, protocols for dual-inlet isotope ratio mass spectrometry, laser-capture microdissection, and bioinformatics approach for exploring the co-regulation of AOX gene family members. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Plant Respiration and Internal Oxygen: Methods and Protocols aims to be helpful for all students and researchers interested in the determination of respiration and internal oxygen. 

Editors and Affiliations

  • National Institute of Plant Genome Research, New Delhi, India

    Kapuganti Jagadis Gupta

Bibliographic Information

  • Book Title: Plant Respiration and Internal Oxygen

  • Book Subtitle: Methods and Protocols

  • Editors: Kapuganti Jagadis Gupta

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7292-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2017

  • Hardcover ISBN: 978-1-4939-7291-3Published: 05 September 2017

  • Softcover ISBN: 978-1-4939-8444-2Published: 03 August 2018

  • eBook ISBN: 978-1-4939-7292-0Published: 04 September 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 285

  • Number of Illustrations: 16 b/w illustrations, 58 illustrations in colour

  • Topics: Plant Sciences

Buy it now

Buying options

eBook USD 119.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access